TY - JOUR A1 - Feifel, Sven Christian A1 - Lisdat, Fred T1 - Silica nanoparticles for the layer-by-layer assembly of fully electro-active cytochrome c multilayers TI - Journal of Nanobiotechnology N2 - For bioanalytical systems sensitivity and biomolecule activity are critical issues. The immobilization of proteins into multilayer systems by the layer-by-layer deposition has become one of the favorite methods with this respect. Moreover, the combination of nanoparticles with biomolecules on electrodes is a matter of particular interest since several examples with high activities and direct electron transfer have been found. Our study describes the investigation on silica nanoparticles and the redox protein cytochrome c for the construction of electro-active multilayer architectures, and the electron transfer within such systems. The novelty of this work is the construction of such artificial architectures with a non-conducting building block. Furthermore a detailed study of the size influence of silica nanoparticles is performed with regard to formation and electrochemical behavior of these systems. Y1 - 2016 UR - https://opus4.kobv.de/opus4-th-wildau/frontdoor/index/index/docId/581 UR - https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-5819 SN - 1477-3155 VL - 9 IS - 59 ER -