TY - JOUR A1 - Devulapally, Praneeth Reddy A1 - Bürger, Jörg A1 - Mielke, Thorsten A1 - Konthur, Zoltán A1 - Lehrach, Hans A1 - Yaspo, Marie-Laure A1 - Glökler, Jörn A1 - Warnatz, Hans-Jörg T1 - Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes TI - Genome Medicine N2 - Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies. Y1 - 2018 UR - https://opus4.kobv.de/opus4-th-wildau/frontdoor/index/index/docId/1053 UR - https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-10538 SN - 1756-994X VL - 10 SP - 1 EP - 12 ER -