TY - JOUR A1 - Sandig, Grit A1 - von Horsten, Hans Henning A1 - Radke, Lars A1 - Blanchard, Véronique A1 - Frohme, Marcus A1 - Giese, Christoph A1 - Sandig, Volker A1 - Hinderlich, Stephan T1 - Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans TI - Bioengineering N2 - Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of respiratory tract infection in infants and elderly, as a model protein for vaccination. The RSV-F protein was expressed in CHO-DG44 cells, which were further modified by co-expression of β1,2-xylosyltransferase from Nicotiana tabacum. Xylosylation of RSV-F N-glycans was shown by monosaccharide analysis and MALDI-TOF mass spectrometry. In immunogenic studies with a human artificial lymph node model, the engineered RSV-F protein revealed improved vaccination efficacy. KW - CHO KW - glycoengineering KW - respiratory syncytial virus KW - vaccine KW - xylose Y1 - 2017 UR - https://opus4.kobv.de/opus4-th-wildau/frontdoor/index/index/docId/962 UR - https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-9620 SN - 2306-5354 VL - 4 IS - 2 ER -