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The conversion efficiency of high solids waste digestion as sugarcane press mud (P) may be limited due to hydrolysis step. The option of co-digestion with vinasse, main liquid waste generated from ethanol production, was investigated under batch regime at mesophilic conditions (37.5 ± 1 °C) and the best mixture was evaluated under semicontinuous regime in stirred-tank reactors. The maximum values for methane yield in batch tests were for V75/P25 and V50/P50 mixtures (on basis of the chemical oxygen demand (COD) percentage added in the mixture), with an average value of 246 N mL CH4 g−1 CODfed, which was 13% higher than that of press mud alone. A highest methane production rate of 69.6 N mL CH4 g−1 CODfed−1 d−1 was obtained for the mixtureV75/P25. During the experiment carried out in CSTR reactors, the organic loading rate (OLR) was increased from 0.5 up to 2.2 g VS L−1 d−1. Methane yields of 365 L CH4 kg−1 VS and biogas productivities of 1.6 L L−1 were obtained in co-digestion, which was 64% higher in comparison to mono-digestion. The performance of the process in mono-digestion was less stable than in co-digestion, with a significant fall of methane yield to 1.8 kg VS m−3 d−1, and a partial inhibition of the methanogenic archaeas when the OLR was increased up to 2.2 kg VS m−3 d−1. The co-digestion of vinasse with press mud is a good option for the treatment of streams at the alcohol-sugar industry.