TY - CHAP A1 - Schwarz, Jonas A1 - Wohlschläger, Maximilian A1 - Leiter, Nina A1 - Auer, Veronika A1 - Risse, Michael A1 - Versen, Martin T1 - Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM) analysis of Quercus robur samples for origin differentiation purposes T2 - Optica Sensing Congress 2023 (AIS, FTS, HISE, Sensors, ES) N2 - Increasing demand for wood products requires methods to determine its harvest origin and ensure sustainable and legal sourcing. In 15 out of 21 cases, the origin of Quercus robur was differentiable in FD-FLIM studies. KW - Fluorescence lifetime imaging KW - Phase shift KW - Phase modulation KW - Laser sources KW - Bandpass filters KW - Frequency modulation Y1 - 2023 U6 - https://doi.org/10.1364/AIS.2023.JTu4A.10 ER - TY - CHAP A1 - Leiter, Nina A1 - Schwarz, Jonas A1 - Versen, Martin A1 - Risse, Michael A1 - Löder, Martin G.J. A1 - Laforsch, Christian T1 - A non-destructive approach to wood origin differentiation using FD-FLIM T2 - 2024 IEEE Sensors Applications Symposium (SAS) N2 - Wood auto-fluorescence, primarily attributed to lignin, presents a distinctive feature. Different wood species exhibit variations in lignin distribution. Frequency-Domain Fluorescence Lifetime Imaging Microscopy is effective in distinguishing wood species based on their fluorescence characteristics. This study investigates the potential to differentiate the origins of beech, spruce, and larch through phase-dependent fluorescence decay times. Therefore the zero hypothesis H0 is tested: The phase dependent fluorescence lifetimes of samples from the same species but varying in origin are equal. To determine the fluorescence characteristics of woods of different origins, wood samples of the species Fagus sylvatica L. (beech), Larix decidua Mill. (larch), and Picea abies (L.) H. Karst. (spruce) from Germany, Austria, the Netherlands, Spain, Sweden, New Zealand and Romania were analyzed. The wood samples were analyzed with a FD-FLIM camera setup, including a laser source emitting at an excitation wavelength of 445 nm. Employing Analysis-of-Variance hypothesis testing on fluorescence lifetime data for each wood species, the results indicate that 23 out of 35 origin pairs could be distinguished at a 5% significance level. While acknowledging the challenges of origin-based differentiation, the findings emphasize the promising potential of fluorescence lifetime imaging microscope as a valuable tool in this context. Moving forward, a more intricate approach to sample differentiation should involve acquiring detailed information about the samples, including associated temperature and precipitation profiles, and soil composition. KW - wood KW - fluorescence KW - FLIM KW - origin KW - species Y1 - 2024 U6 - https://doi.org/10.1109/SAS60918.2024.10636550 SP - 1 EP - 6 PB - IEEE ER -