3073 2025 eng 3651 3663 13 16 417 article 0 -- -- -- Plastics have become an integral part of modern life, and linked to that fact, the demand for and global production of plastics are still increasing. However, the environmental pollution caused by plastics has reached unprecedented levels. The accumulation of small plastic fragments—microplastics and nanoplastics—potentially threatens organisms, ecosystems, and human health. Researchers commonly employ non-destructive analytical methods to assess the presence and characteristics of microplastic particles in environmental samples. However, these techniques require extensive sample preparation, which represents a significant limitation and hinders a direct on-site analysis. In this context, previous investigations showed the potential of fluorescence lifetime imaging microscopy (FLIM) for fast and reliable identification of microplastics in an environmental matrix. However, since microplastics receive an environmental coating after entering nature, a challenge arises from organic contamination on the surface of microplastic particles. How this influences the fluorescence signal and the possibility of microplastic detection are unknown. To address this research gap, we exposed acrylonitrile butadiene styrene (ABS) and polyethylene terephthalate (PET) plastic samples to peptides, proteins, bacteria, and a filamentous fungus to induce organic contamination and mimic environmental conditions. We analyzed the fluorescence spectra and lifetimes of the samples using fluorescence spectroscopy and frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM), respectively. Our results demonstrate that reliably identifying and differentiating ABS and PET was possible via FD-FLIM, even in the presence of these biological contaminations. These findings highlight the potential of this technique as a valuable tool for environmental monitoring and plastic characterization, offering a rapid and efficient alternative to currently used analytical methods. Analytical and Bioanalytical Chemistry 10.1007/s00216-025-05888-y true {"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T05:40:03Z","timestamp":1751089203307,"version":"3.41.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"16","license":[{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["FKZ 031B0828A"],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["391977956 \u2013 SFB 1357"],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Technische Hochschule Rosenheim"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n <jats:p>Plastics have become an integral part of modern life, and linked to that fact, the demand for\u00a0and global production of plastics are still increasing. However, the environmental pollution caused by plastics has reached unprecedented levels. The accumulation of small plastic fragments\u2014microplastics and nanoplastics\u2014potentially threatens organisms, ecosystems, and human health. Researchers commonly employ non-destructive analytical methods to assess the presence and characteristics of microplastic particles in environmental samples. However, these techniques require extensive sample preparation, which represents a significant limitation and hinders a direct on-site analysis. In this context, previous investigations showed the potential of fluorescence lifetime imaging microscopy (FLIM) for fast and reliable identification of microplastics in an environmental matrix. However, since microplastics receive an environmental coating after entering nature, a challenge arises from organic contamination on the surface of microplastic particles. How this influences the\u00a0fluorescence signal and the possibility of microplastic detection are unknown. To address this research gap, we exposed acrylonitrile butadiene styrene (ABS) and polyethylene terephthalate (PET) plastic samples to peptides, proteins, bacteria, and a filamentous fungus to induce organic contamination and mimic environmental conditions. We analyzed the fluorescence spectra and lifetimes of the samples using fluorescence spectroscopy and frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM), respectively. Our results demonstrate that reliably identifying and differentiating ABS and PET was possible via FD-FLIM, even in the presence of these biological contaminations. These findings highlight the potential of this technique as a valuable tool for environmental monitoring and plastic characterization, offering a rapid and efficient alternative to currently used analytical methods.<\/jats:p>\n <jats:p>\n <jats:bold>Graphical Abstract<\/jats:bold>\n <\/jats:p>","DOI":"10.1007\/s00216-025-05888-y","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T17:51:37Z","timestamp":1746813097000},"page":"3651-3663","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of defined organic layers on the fluorescence lifetime of plastic materials"],"prefix":"10.1007","volume":"417","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6472-4255","authenticated-orcid":false,"given":"Nina","family":"Leiter","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2690-2788","authenticated-orcid":false,"given":"Maximilian","family":"Wohlschl\u00e4ger","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4302-2184","authenticated-orcid":false,"given":"Martin","family":"Versen","sequence":"additional","affiliation":[]},{"given":"Sonja D.","family":"Harter","sequence":"additional","affiliation":[]},{"given":"Tina","family":"Kie\u00dflich","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0452-3242","authenticated-orcid":false,"given":"Franziska","family":"Lederer","sequence":"additional","affiliation":[]},{"given":"Stefanie","family":"Clau\u00df","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1353-8136","authenticated-orcid":false,"given":"Dietmar","family":"Schlosser","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6724-0814","authenticated-orcid":false,"given":"Emanuel Gheorghita","family":"Armanu","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8269-5525","authenticated-orcid":false,"given":"Christian","family":"Eberlein","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3723-9600","authenticated-orcid":false,"given":"Hermann J.","family":"Heipieper","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9056-8254","authenticated-orcid":false,"given":"Martin G. 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Harter Tina Kießlich Franziska Lederer Stefanie Clauß Dietmar Schlosser Emanuel Gheorghita Armanu Christian Eberlein Hermann J. Heipieper Martin G. J. Löder Christian Laforsch