<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>390</id>
    <completedYear/>
    <publishedYear>2019</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>76</pageNumber>
    <edition/>
    <issue/>
    <volume/>
    <type>bachelorthesis</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2019-07-16</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>2019-06-12</thesisDateAccepted>
    <title language="eng">Production and detection of polyhydroxybutyrate by Azohydromonas lata</title>
    <abstract language="eng">The aim of the project was to produce, identify and quantify PHB. The bacterial strain Azohydromonas lata was cultured in multiple batch processes and the accumulation of biopolymer was observed. The following results were obtained after 50 hours of a 3L batch fermentation: a dry cell weight of 3.197 g/L, a PHB concentration of 1.939 g/L and a PHB content of 60.65%. Subsequently, the same set-up was extrapolated to an 8L batch fermentation, which yielded in a dry cell weight, PHB concentration and PHB content of 2.964 g/L, 1.090 g/L and 36.79%, respectively. The synthesized biopolymer was recovered from the bacterial cells in two ways, namely by SDS digestion and by chloroform Soxhlet extraction. Afterwards, three analytical methods were tested for detecting and quantifying the produced PHB. Fermentation samples stained with the Nile Red dye were analysed with both fluorescence microscopy and spectrofluorometry, and the presence of PHB granules could be confirmed. Both methods showed promising results, but further optimization is still necessary. Finally, the chloroform extracted PHB samples were successfully analysed using HPLC. The HPLC method proved to reliably identify and to relatively accurately quantify the recovered PHB.</abstract>
    <identifier type="urn">urn:nbn:de:hbz:1383-opus4-3905</identifier>
    <licence>CC BY 4.0 International - Namensnennung</licence>
    <author>Felicia Zlati</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biopolymer</value>
    </subject>
    <collection role="institutes" number="">Fakultät Life Sciences</collection>
    <thesisPublisher>Hochschule Rhein-Waal</thesisPublisher>
    <thesisGrantor>Hochschule Rhein-Waal</thesisGrantor>
    <file>https://opus4.kobv.de/opus4-rhein-waal/files/390/Thesis_Felicia_Zlati.pdf</file>
  </doc>
</export-example>
