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  <doc>
    <id>2215</id>
    <completedYear/>
    <publishedYear>2025</publishedYear>
    <thesisYearAccepted/>
    <language>deu</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>researchdata</type>
    <publisherName/>
    <publisherPlace/>
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    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2025-10-31</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="deu">Unlocking the Power of Short Cycles: Enhancing Hygiene in Domestic Dishwashers</title>
    <abstract language="eng">This study investigated how factors associated with Sinner’s principle—namely temperature, time and the chemical composition of detergents—affected the antimicrobial efficacy of domestic dishwashers, particularly during short cycles. We chose a range of bacterial strains, including standard test strains such as Micrococcus luteus and Enterococcus faecium, as well as important foodborne pathogens such as Escherichia coli, Staphylococcus aureus, and Salmonella Typhimurium. To account for the complexity of dishwasher cycles, we correlated hygiene efficacy with area under the curve (AUC) measurements derived from the respective cycle profiles. Our findings revealed that the reductions in M. luteus and E. faecium were minimally affected by the reference detergent. In contrast, a high-tier market detergent demonstrated a significant decrease in bacterial counts. Notably, both strains exhibited reduced efficacy at a main cycle temperature of 45 °C, suggesting that temperatures below 50 °C might represent a critical threshold at which the hygiene efficacy of domestic dishwashing processes declines. However, since food-related pathogens were more susceptible to the dishwashing process, even lower temperatures might deliver a sufficient level of hygiene. Plotting the logarithmic reduction/AUC ratio against the AUC indicated that the main cycle contributed approximately 10-fold more to microbial reduction than the rinse cycle. Furthermore, the antimicrobial impact of detergents was greatest at the lowest AUC values (i.e. during short cycles). Taken together, our results suggest that the main wash temperature, the type of detergent, and the specific strain tested all impact bacterial hygiene performance.</abstract>
    <identifier type="urn">urn:nbn:de:hbz:1383-opus4-22153</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
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    <licence>CC0 1.0 Universell - Gemeinfreiheit (Public Domain)</licence>
    <author>Matthias Kudla</author>
    <author>Thomas Tewes</author>
    <author>Emma Gibbin-Lameira</author>
    <author>Laurence Harcq</author>
    <author>Dirk Bockmühl</author>
    <collection role="institutes" number="">Fakultät Life Sciences</collection>
    <thesisPublisher>Hochschule Rhein-Waal</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-rhein-waal/files/2215/Research-data-Matthias-Kudla.zip</file>
  </doc>
  <doc>
    <id>2082</id>
    <completedYear/>
    <publishedYear>2024</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>researchdata</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
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    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2024-10-31</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Does antibiotic use contribute to biofilm resistance in sink drains? A case study from four German hospital wards.</title>
    <abstract language="eng">As biofilms are known to harbour (multi-)resistant species, their presence in health settings must be considered critical. Although there is evidence that bacteria spread from drains to the outside, there is still a lack of research data focusing on drain biofilms from hospitals.&#13;
We sampled biofilms from various wards of Helios Hospital Krefeld (Germany), where comprehensive antibiotic consumption data were available. Biofilms were analysed by cell counting, isolation of relevant bacterial groups and genetic and phenotypical resistance parameters. Data were correlated with the prescribed antibiotics of the respective ward. Furthermore, an ex-situ biofilm model was employed to investigate the influence of sub-inhibitory antibiotics on the bacterial community and the prevalence of class 1 integrons.&#13;
Our results show, that every ward harboured medically relevant bacterial species. While no significant differences were found in cell counts, the median prevalence of the resistance marker gene intI1 correlated with the amount of prescribed antibiotics. In contrast, phenotypical resistances showed no similar tendency. In addition, melting curve analysis data and changes in intI1 prevalence show that the composition of the bacterial community shifted depending on the biofilm and antibiotic.&#13;
Based on our results we conclude, that sub-inhibitory concentrations of antibiotics have no general effect on biofilms in terms of bacterial community shift and occurrence of antibiotic resistant species. The effect depends on the initial composition of the bacterial community, the antibiotic used and the intrinsic bacterial resistance e.g. prevalence of class 1 integrons.</abstract>
    <identifier type="urn">urn:nbn:de:hbz:1383-opus4-20820</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <licence>CC BY-NC-ND 4.0 International - Namensnennung-Nicht kommerziell-Keine Bearbeitungen</licence>
    <author>Nicole van Leuven</author>
    <author>Ralf Lucassen</author>
    <author>Anna Dicks</author>
    <author>Patrick Braß</author>
    <author>André Lipski</author>
    <author>Dirk Bockmühl</author>
    <collection role="ddc" number="57">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fakultät Life Sciences</collection>
    <thesisPublisher>Hochschule Rhein-Waal</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-rhein-waal/files/2082/SequencingResults.zip</file>
  </doc>
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