@masterthesis{Seidenberg, type = {Bachelor Thesis}, author = {Seidenberg, Viola}, title = {Investigation and validation of a drop-on demand bioprinting process for biofabrication of hMSC and HUVEC laden fibrin structures}, school = {Hochschule Rhein-Waal}, abstract = {3D bioprinting is an emerging field of tissue engineering that has great potential to change the picture in personalized medicine and pharmaceutical research. But there is still a long way to go until bioprinting processes become commercially realizable. In this work, a newly developed bioprinting process for the production of hMSC and HUVEC laden fibrin structures, using a custom-built microvalve-based bioprinter, was investigated. The gelation behaviour of fibrin, as well as the droplet deposition process were analysed in detail in order to optimize the printing process. Furthermore, a strategy was developed to print drops of a gelatin agarose mixture into consecutive layers of fibrin which showed to improve the mechanical stability of the printed constructs and to promote cell growth in culture for at least fourteen days. Additionally, endothelial cells were found to develop vascular structures in the cultured samples which is crucial for the construction of bigger functional engineered tissues.}, language = {en} } @masterthesis{Kim, type = {Bachelor Thesis}, author = {Kim, Wooyong}, title = {3D bioprinting of cartilage tissue: Current advances and challenges in tissue engineering}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:1383-opus4-15373}, school = {Hochschule Rhein-Waal}, pages = {92}, abstract = {As industrial technologies develop, some techniques were adopted and applied in medical fields for treatment improvement. Among them, 3D bioprinting is regarded as a rising and trending technology in the tissue engineering area. This is because not only does it offer patients customized organs or tissues, but also it can lower animal test dependency on drug testing. This paper is mainly focused on the use of 3D printing as a cartilage tissue fabrication for transplantation. In the beginning, general information and background knowledge concerning cartilage and bioprinting approaches were addressed. Subsequently, current research results from eight research teams related to cartilage tissue printing and grafting were introduced. Then the reports were classified and sorted into several groups to analyze and evaluate various methods' properties, advantages, and disadvantages. After that, some classic and traditional ways to produce artificial tissues without 3D bioprinting techniques were described to prove the strength of the novel tissue engineering technology. In the end, several side points, such as limitations, 3D bioprinting usages in fields other than tissue transplantation, market value, and outlook of bioprinting, were discussed. Information and sources were retrieved using Google and PubMed database search engines under specific inclusion and exclusion criteria through a PCC concept. Although the 3D bioprinting technique is still in development processes, it has a high potential to be a game-changing approach in the medical and pharmaceutical fields after commercialization in the future.}, language = {en} }