@masterthesis{Mumtaz, type = {Bachelor Thesis}, author = {Mumtaz, Muhammad Imran}, title = {Generation and expression of a mutated murine tRNA-guanine transglycosylase variant}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:1383-opus4-18560}, school = {Hochschule Rhein-Waal}, pages = {70}, abstract = {Translation of messenger RNA carrying the nucleic code for protein synthesis is one of the vital bioprocesses of living organisms. This translation is mediated by transfer RNA, which allocates specific amino acids to the growing chain by decoding the genetic code. The tRNAs with the G34U35N36 anticodon sequence are recognised by an enzyme called tRNA-guanine transglycosylase, which catalyses the exchange of guanine with queuine. TGT enzyme is found in all three domains of life and is involved in post-transcriptional modification. TGT is of keen interest in the development of therapeutics against shigellosis, since the genetic inactivation of TGT leads to decreased pathogenicity in Shigella. For understanding the differences between bacterial and eukaryotic TGT, studies have been performed in the past, which show remarkable resemblance. On the contrary, some pronounced structural contrast can also be observed. One prominent difference is that the bacterial TGT contains a short turn of three amino acids, whereas, in eukaryotes, this turn is replaced by an extended loop of more than 45 amino acids. The function of this loop in eukaryotic TGT is not known. The loop is a highly flexible region and, thus, is not structurally solved. To elucidate the function of this loop, a TGT mutant lacking this loop was generated, expressed and purified. TGT mutant was expressed in different bacterial cell strains. For the purification, different chromatography techniques were utilised. The mutation resulted in a low yield of the protein in comparison to the wild-type TGT. This leads to the inference that the loop influences the physicochemical characteristics of the protein.}, language = {en} } @masterthesis{May, type = {Bachelor Thesis}, author = {May, Clarissa}, title = {Efficacy of Traditional Chinese Medical DrugsThe Treatment of Common Illnesses in Comparison with Western medicine}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:1383-opus4-19440}, school = {Hochschule Rhein-Waal}, pages = {78}, abstract = {The efficacy of treating common illnesses with traditional Chinese medical drugs has been a subject of growing interest and debate especially when compared to established practices of modern Western medicine. The integration of alternative and traditional medicine practices also gains significance as healthcare systems evolve. This literature review aims to comprehensively assess and compare the effectiveness of traditional Chinese medical drugs with modern Western medial drugs in addressing common illnesses. Through a systematic analysis of reviewed scientific articles, clinical studies, and relevant literature the effectiveness of traditional Chinese medical drugs will be identified and compared to modern Western medical drugs and treatment options in context of the treatment success rates, symptom relief, adverse effects, and patient satisfaction. The study focuses on the herbal based remedies and their traditional therapeutic treatment methods, to illuminate their mechanisms of action and potential therapeutic benefits for a range of common illnesses such as the common cold and flu, digestive disorders, musculoskeletal pain, and skin conditions. Furthermore, the potential synergies between the two practices will be explored, as integrative healthcare becomes increasingly important as a comprehensive approach to patient wellbeing. Through a comprehensive analysis of the available medical literature and clinical data, this paper attempts to address the potential of traditional Chinese medical drugs as an alternative or complementary method to modern Western medicine for various common illnesses. The findings may contribute to informed decision-making by healthcare professionals and patients, offering insights into the strengths and limitations of each approach. Additionally, the research aims to foster a deeper understanding of cross-cultural medical practices and their potential integration into contemporary healthcare systems.}, language = {en} } @masterthesis{Alshaikh, type = {Bachelor Thesis}, author = {Alshaikh, Badran-Mohammednoor}, title = {Medi: A Software System for Improving the Analysis and Interpretation of Medical Tests in Clinical Laboratories.}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:1383-opus4-19466}, school = {Hochschule Rhein-Waal}, pages = {77}, abstract = {The rapid increase in patient numbers and the complexity of clinical information, coupled with a significant lack of assistive technologies for routine procedures, has emphasized the urgent need for innovative technological solutions in clinical laboratories. This need is amplified by the existence of over 238 blood tests, highlighting the challenge of managing and interpreting vast amounts of clinical information and data efficiently. The primary objective of this research is to evaluate the effectiveness of Medi, a state-of-the-art software system designed to enhance the workflow of medical routine tasks in the pre-testing steps in clinical laboratories. By focusing on routine hematology, biochemistry, and microbiology tests, the study aims to revolutionize clinical laboratory processes, improve diagnostic accuracy, and enhance patient care. Through an extensive approach regarding specimen collection of blood and the utilization of automated medical devices, this study sheds light on the advantage of Medi to streamline operations within clinical laboratories. The research methodically assesses the impact of Medi on laboratory workflows and patient outcomes, employing a user-centric design to ensure the software meets the practical needs of healthcare professionals. Medi demonstrated significant improvements in laboratory efficiency, with streamlined workflow. Key findings include the standardization of blood specimen collection procedures, limiting blood-tube-selection errors, and a positive impact on laboratory operations. The software facilitated a more cohesive integration of clinical data, contributing to better-informed decision-making and improved patient care. By comparing these outcomes with the current state of clinical diagnostics, Medi's introduction represents a transformative shift in laboratory operations. It not only addresses the pressing need for advanced assistive technologies in the clinical laboratory settings but also sets a new standard in patient care. This research underlines the potential of integrating software solutions with existing laboratory technologies, paving the way for a new era in clinical testing.}, language = {en} }