@masterthesis{Babenko, type = {Bachelor Thesis}, author = {Babenko, Anastasiia}, title = {Development of an ESI-MS/MS-based method for the quantification of acylcarnitines in murine tissue}, school = {Hochschule Rhein-Waal}, abstract = {Acylcarnitines serve as transporters of activated fatty acids into mitochondria for β-oxidation - the major source of the cell's energy and are considered as indicative markers for various metabolic disorders. The investigation of acylcarnitines in cell and tissue samples is a particularly complicated task due to their low abundance and high heterogeneity. This requires applications of sophisticated lipid separation methods. The purpose of this work was the development of an ESI-MS/MS method for the quantification of acylcarnitines with high specificity and reliability for the identification of low abundant acylcarnitine species in complex samples. General aspects of acylcarnitine analysis, including several types of solid-phase extraction, and derivatization with liquid extraction were investigated using commercially available acylcarnitine standards. The established experimental workflows, including an optimized cation-exchange extraction and derivatization with liquid extraction, were successfully applied to quantify acylcarnitine species in samples of biological origin. Consequently, murine brain tissue samples were quantitatively analyzed using two extraction procedures. The method developed in the study can be used for the qualitative and quantitative analysis of acylcarnitines in different biological samples.}, language = {en} }