@masterthesis{Sadeghi, type = {Bachelor Thesis}, author = {Sadeghi, Yasaman}, title = {Fermentation production process of hyaluronic acid}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:1383-opus4-16618}, school = {Hochschule Rhein-Waal}, pages = {58}, abstract = {Hyaluronic acid is an anionic and naturally produced polysaccharide with an unbranched formation, with two alternating disaccharide units (β-1, 3-N-acetyl glucosamine, and β-1, 4-glucuronic acid), which has various medical and pharmaceutical applications due to its wide variety of unique and natural functions and characteristics. Hyaluronan is essential in treating joint diseases by reducing inflammation in osteoarthritic joints, from which so many people suffer worldwide, and the demand for this product is increasing. The industrial production of hyaluronic acid has improved over time. The industrial production process is primarily based on the extraction of hyaluronic acid from animal tissues and the biotechnological application of microorganisms for hyaluronic acid biosynthesis that produce high molecular weight hyaluronan molecules. Using microorganisms is the most favorable method for large-scale hyaluronic acid production. This study focuses on designing hyaluronic acid production using Streptococcus zooepidemicus and SuperPro Designer to obtain a suitable yield of injectable HA to treat knee-related problems. Two cases have been developed. Case A is the baseline scenario, mainly focused on producing injectable hyaluronic acid, and case B is an elevated and optimized scenario to achieve a higher amount of injectable HA. Both processes include upstream processing, primary recovery, purification, production of injectable hyaluronan, and isopropanol distillation. The upstream section contains the fermentation process and the inoculum preparation by seed training. The fermentation step was set to work in fed-batch mode, and aeration was applied to increase the final yield. The primary recovery section encompasses crossflow filtration. This process starts with the centrifugation of the broth in disk-stack centrifugation, and purification includes Granular Activated Carbon adsorption, isopropanol precipitation, basket centrifugation, diafiltration, spray drying, and freeze-drying. Ultimately, the cost of raw materials, labor, equipment purchase, annual operating, waste treatment, and utilities are analyzed in the economic evaluation. The selling price was estimated to be \$2000 per kg of HA for the cosmetic industry and \$50,000 per kg for injections. Additionally, sensitivity analysis is performed to predict the outcome of any changes in a variable and assist the producers in decision-making processes.  }, language = {en} }