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Ultra-Soft PDMS-Based Magnetoactive Elastomers as Dynamic Cell Culture Substrata

  • Mechanical cues such as extracellular matrix stiffness and movement have a major impact on cell differentiation and function. To replicate these biological features in vitro, soft substrata with tunable elasticity and the possibility for controlled surface translocation are desirable. Here we report on the use of ultra-soft (Young’s modulus <100 kPa) PDMS-based magnetoactive elastomers (MAE) as suitable cell culture substrata. Soft non-viscous PDMS (<18 kPa) is produced using a modified extended crosslinker. MAEs are generated by embedding magnetic microparticles into a soft PDMS matrix. Both substrata yield an elasticity-dependent (14 vs. 100 kPa) modulation of α-smooth muscle actin expression in primary human fibroblasts. To allow for static or dynamic control of MAE material properties, we devise low magnetic field (≈40 mT) stimulation systems compatible with cell-culture environments. Magnetic field-instigated stiffening (14 to 200 kPa) of soft MAE enhances the spreading of primary human fibroblasts and decreases PAX-7 transcription in human mesenchymal stem cells. Pulsatile MAE movements are generated using oscillating magnetic fields and are well tolerated by adherent human fibroblasts. This MAE system provides spatial and temporal control of substratum material characteristics and permits novel designs when used as dynamic cell culture substrata or cell culture-coated actuator in tissue engineering applications or biomedical devices.

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Metadaten
Author:Matthias Mayer, Raman Rabindranath, Juliane Börner, Eva Hörner, Alexander Bentz, Josefina Salgado, Hong Han, Holger Böse, Jörn Probst, Mikhail Shamonin (Chamonine)ORCiDGND, Gareth J. MonkmanORCiDGND, Günther Schlunck
DOI:https://doi.org/10.1371/journal.pone.0076196
Parent Title (English):PLoS ONE
Publisher:PLOS
Document Type:Article
Language:English
Year of first Publication:2013
Release Date:2022/12/10
Volume:8
Issue:10
Article Number:e76196
Institutes:Fakultät Elektro- und Informationstechnik
Fakultät Elektro- und Informationstechnik / Labor Intelligente Materialien und Strukturen
Fakultät Elektro- und Informationstechnik / Mechatronics Research Unit (MRU)
Begutachtungsstatus:peer-reviewed
OpenAccess Publikationsweg:Gold Open Access- Erstveröffentlichung in einem/als Open-Access-Medium
research focus:Produktion und Systeme
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International