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Quantitative Analysis of Surface Contouring with Pulsed Bipolar Radiofrequency on Thin Chondromalacic Cartilage

  • The purpose of this study was to evaluate the quality of surface contouring of chondromalacic cartilage by bipolar radio frequency energy using different treatment patterns in an animal model, as well as examining the impact of the treatment onto chondrocyte viability by two different methods. Our experiments were conducted on 36 fresh osteochondral sections from the tibia plateau of slaughtered 6-month-old pigs, where the thickness of the cartilage is similar to that of human wrist cartilage. An area of 1 cm(2) was first treated with emery paper to simulate the chondromalacic cartilage. Then, the treatment with RFE followed in 6 different patterns. The osteochondral sections were assessed for cellular viability (live/dead assay, caspase (cell apoptosis marker) staining, and quantitative analysed images obtained by fluorescent microscopy). For a quantitative characterization of none or treated cartilage surfaces, various roughness parameters were measured using confocal laser scanning microscopy (Olympus LEXT OLS 4000 3D). To describe the roughness, the Root-Mean-Square parameter (Sq) was calculated. A smoothing effect of the cartilage surface was detectable upon each pattern of RFE treatment. The Sq for native cartilage was Sq=3.8 +/- 1.1 mu m. The best smoothing pattern was seen for two RFE passes and a 2-second pulsed mode (B2p2) with an Sq=27.3 +/- 4.9 mu m. However, with increased smoothing, an augmentation in chondrocyte death up to 95% was detected. Using bipolar RFE treatment in arthroscopy for small joints like the wrist or MCP joints should be used with caution. In the case of chondroplasty, there is a high chance to destroy the joint cartilage.

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Metadaten
Author:Michaela HuberORCiD, Daniela Schlosser, Susanne Stenzel, Johannes Maier, Girish PattappaORCiD, Richard KujatORCiD, Birgit Striegl, Denitsa Docheva
DOI:https://doi.org/10.1155/2020/1242086
Parent Title (English):BioMed Research International
Publisher:HINDAWI
Document Type:Article
Language:English
Year of first Publication:2020
Release Date:2022/01/31
Tag:CHONDROCYTE DEATH; HUMAN ARTICULAR-CARTILAGE; MONOPOLAR; THERMAL CHONDROPLASTY; energy
Article Number:1242086
First Page:1
Last Page:8
Institutes:Fakultät Informatik und Mathematik
Regensburg Center of Biomedical Engineering - RCBE
Fakultät Informatik und Mathematik / Regensburg Medical Image Computing (ReMIC)
Begutachtungsstatus:peer-reviewed
OpenAccess Publikationsweg:Gold Open Access- Erstveröffentlichung in einem/als Open-Access-Medium
research focus:Lebenswissenschaften und Ethik
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International