Quantifying the A1AR distribution in peritumoral zones around experimental F98 and C6 rat brain tumours

  • Quantification of growth in experimental F98 and C6 rat brain tumours was performed on 51 rat brains, 17 of which have been further assessed by 3D tumour reconstruction. Brains were cryosliced and radio-labelled with a ligand of the peripheral type benzodiazepine-receptor (pBR), 3H-Pk11195 [(1-(2-chlorophenyl)-N-methyl-N-(1-methyl-propylene)-3-isoquinoline-carboxamide)] by receptor autoradiography. Manually segmented and automatically registered tumours have been 3D-reconstructed for volumetric comparison on the basis of 3H-Pk11195-based tumour recognition. Furthermore automatically computed areas of −300 μm inner (marginal) zone as well as 300 μm and 600 μm outer tumour space were quantified. These three different regions were transferred onto other adjacent slices that had been labelled by receptor autoradiography with the A1 Adenosine receptor (A1AR)-ligand 3H-CPFPX (3H-8-cyclopentyl-3-(3-fluorpropyl)-1-propylxanthine) for quantitative assessment of A1AR in the three different tumour zones. Hence, a method is described for quantifying various receptor protein systems in the tumour as well as in the marginal invasive zones around experimentally implanted rat brain tumours and their representation in the tumour microenvironment as well as in 3D space. Furthermore, a tool for automatically reading out radio-labelled rat brain slices from auto radiographic films was developed, reconstructed into a consistent 3D-tumour model and the zones around the tumour were visualized. A1AR expression was found to depend upon the tumour volume in C6 animals, but is independent on the time of tumour development. In F98 animals, a significant increase in A1AR receptor protein was found in the Peritumoural zone as a function of time of tumour development and tumour volume.

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Author:Markus Dehnhardt, Christoph PalmORCiDGND, Andrea Vieten, Andreas Bauer, Uwe Pietrzyk
Parent Title (English):Journal of Neuro-Oncology
Document Type:Article
Year of first Publication:2007
Release Date:2020/01/22
Tag:3D reconstruction; A1 adenosine receptor; Brain tumour; GBM; Kmeans algorithm; Receptor autoradiography
GND Keyword:Hirntumor; Dreidimensionale Bildverarbeitung; Adenosinrezeptor
First Page:49
Last Page:63
Institutes:Fakultät Informatik und Mathematik
Fakultät Informatik und Mathematik / Regensburg Medical Image Computing (ReMIC)
Publication:Externe Publikationen
research focus:Lebenswissenschaften und Ethik