TY - JOUR A1 - Matusch, Andreas A1 - Depboylu, Candan A1 - Palm, Christoph A1 - Wu, Bei A1 - Höglinger, Günter U. A1 - Schäfer, Martin K.-H. A1 - Becker, Johanna Sabine T1 - Cerebral bio-imaging of Cu, Fe, Zn and Mn in the MPTP mouse model of Parkinsons disease using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) JF - Journal of the American Society for Mass Spectrometry N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronically intoxicated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) as a model of Parkinson’s disease. Images were calibrated using matrix-matched laboratory standards. A software solution allowing a precise delineation of anatomical structures was implemented. Coronal brain sections were analyzed crossing the striatum and the substantia nigra, respectively. Animals sacrificed 2 h, 7 d, or 28 d after the last MPTP injection and controls were investigated. We observed significant decreases of Cu concentrations in the periventricular zone and the fascia dentata at 2 h and 7d and a recovery or overcompensation at 28 d, most pronounced in the rostral periventricular zone (+40%). In the cortex Cu decreased slightly to −10%. Fe increased in the interpeduncular nucleus (+40%) but not in the substantia nigra. This pattern is in line with a differential regulation of periventricular and parenchymal Cu, and with the histochemical localization of Fe, and congruent to regions of preferential MPTP binding described in the rodent brain. The LA-ICP-MS technique yielded valid and statistically robust results in the present study on 39 slices from 19 animals. Our findings underline the value of routine micro-local analytical techniques in the life sciences and affirm a role of Cu availability in Parkinson’s disease. KW - Inductively Couple Plasma Mass Spectrometry KW - Substantia Nigra KW - MPTP KW - Laser Ablation Inductively Couple Plasma Mass Spectrometry KW - MPTP Treatment KW - ICP-Massenspektrometrie KW - Metalle KW - Gehirnkarte KW - MPTP Y1 - 2010 U6 - https://doi.org/10.1016/j.jasms.2009.09.022 VL - 21 IS - 1 SP - 161 EP - 171 ER - TY - JOUR A1 - Becker, Johanna Sabine A1 - Zoriy, Miroslav A1 - Matusch, Andreas A1 - Wu, Bei A1 - Salber, Dagmar A1 - Palm, Christoph A1 - Becker, Julia Susanne T1 - Bioimaging of Metals by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) JF - Mass Spectrometry Reviews N2 - The distribution analysis of (essential, beneficial, or toxic) metals (e.g., Cu, Fe, Zn, Pb, and others), metalloids, and non‐metals in biological tissues is of key interest in life science. Over the past few years, the development and application of several imaging mass spectrometric techniques has been rapidly growing in biology and medicine. Especially, in brain research metalloproteins are in the focus of targeted therapy approaches of neurodegenerative diseases such as Alzheimer's and Parkinson's disease, or stroke, or tumor growth. Laser ablation inductively coupled plasma mass spectrometry (LA‐ICP‐MS) using double‐focusing sector field (LA‐ICP‐SFMS) or quadrupole‐based mass spectrometers (LA‐ICP‐QMS) has been successfully applied as a powerful imaging (mapping) technique to produce quantitative images of detailed regionally specific element distributions in thin tissue sections of human or rodent brain. Imaging LA‐ICP‐QMS was also applied to investigate metal distributions in plant and animal sections to study, for example, the uptake and transport of nutrient and toxic elements or environmental contamination. The combination of imaging LA‐ICP‐MS of metals with proteomic studies using biomolecular mass spectrometry identifies metal‐containing proteins and also phosphoproteins. Metal‐containing proteins were imaged in a two‐dimensional gel after electrophoretic separation of proteins (SDS or Blue Native PAGE). Recent progress in LA‐ICP‐MS imaging as a stand‐alone technique and in combination with MALDI/ESI‐MS for selected life science applications is summarized. KW - Bildgebendes Verfahren KW - ICP-Massenspektrometrie KW - Metalle KW - Metallproteide KW - Elektrophorese KW - Gehirnkarte KW - Bioimaging of metals KW - Laser ablation inductively coupled plasma mass spectrometry KW - metal distribution KW - metallomics KW - neurodegenerative diseases Y1 - 2010 U6 - https://doi.org/10.1002/mas.20239 VL - 29 SP - 156 EP - 175 ER -