TY - JOUR A1 - Lehle, Karla A1 - Philipp, Alois A1 - Krenkel, Lars A1 - Gruber, Michael A1 - Hiller, Karl-Anton A1 - Müller, Thomas A1 - Lubnow, Matthias T1 - Thrombocytopenia During Venovenous Extracorporeal Membrane Oxygenation in Adult Patients With Bacterial, Viral, and COVID-19 Pneumonia JF - ASAIO Journal N2 - Contact of blood with artificial surfaces triggers platelet activation. The aim was to compare platelet kinetics after venovenous extracorporeal membrane oxygenation (V-V ECMO) start and after system exchange in different etiologies of acute lung failure. Platelet counts and coagulation parameters were analyzed from adult patients with long and exchange-free (≥8 days) ECMO runs (n = 330) caused by bacterial (n = 142), viral (n = 76), or coronavirus disease 2019 (COVID-19) (n = 112) pneumonia. A subpopulation requiring a system exchange and with long, exchange-free runs of the second oxygenator (≥7 days) (n = 110) was analyzed analogously. Patients with COVID-19 showed the highest platelet levels before ECMO implantation. Independent of the underlying disease and ECMO type, platelet counts decreased significantly within 24 hours and reached a steady state after 5 days. In the subpopulation, at the day of a system exchange, platelet counts were lower compared with ECMO start, but without differences between underlying diseases. Subsequently, platelets remained unchanged in the bacterial pneumonia group, but increased in the COVID-19 and viral pneumonia groups within 2–4 days, whereas D-dimers decreased and fibrinogen levels increased. Thus, overall platelet counts on V-V ECMO show disease-specific initial dynamics followed by an ongoing consumption by the ECMO device, which is not boosted by new artificial surfaces after a system exchange. Y1 - 2025 U6 - https://doi.org/10.1097/MAT.0000000000002383 SN - 1058-2916 SN - 1538-943X PB - Wolters Kluwer ER - TY - CHAP A1 - Schulz, Irene A1 - Putzger, Jürgen A1 - Niklas, Andreas A1 - Brandt, M. A1 - Jäger, Andreas A1 - Hardt, A. A1 - Knörzer, S. A1 - Hiller, Karl-Anton A1 - Löffler, S. A1 - Schmalz, Gottfried A1 - Danilov, Sergey N. A1 - Giglberger, Stephan A1 - Hirmer, Marion A1 - Ganichev, Sergey D. A1 - Monkman, Gareth J. T1 - PPG signal acquisition and analysis on in vitro tooth model for dental pulp vitality assessmen T2 - 2nd Applied Research Conference 2012 (ARC 2012), 25./26. June 2012, Nuremberg Y1 - 2012 SP - 142 EP - 146 PB - Shaker-Verlag CY - Aachen ER - TY - CHAP A1 - Niklas, Andreas A1 - Hiller, Karl-Anton A1 - Jäger, Andreas A1 - Brandt, M. A1 - Putzger, Jürgen A1 - Ermer, C. A1 - Schulz, Irene A1 - Monkman, Gareth J. A1 - Giglberger, Stephan A1 - Hirmer, Marion A1 - Danilov, Sergey N. A1 - Ganichev, Sergey D. A1 - Schmalz, Gottfried ED - Arenholt-Bindslev, Dorthe ED - Schmalz, Gottfried T1 - In vitro optical detection of simulated blood pulse in a human tooth pulp model T2 - Biocompatibility of Dental Materials Y1 - 2013 PB - Springer CY - Berlin ; Heidelberg ER - TY - CHAP A1 - Drexler, C. A1 - Hirmer, Marion A1 - Danilov, Sergey N. A1 - Giglberger, Stephan A1 - Putzger, Jürgen A1 - Niklas, Andreas A1 - Jäger, Andreas A1 - Hiller, Karl-Anton A1 - Löffler, S. A1 - Schmalz, Gottfried A1 - Redlich, Britta A1 - Schulz, Irene A1 - Monkman, Gareth J. A1 - Ganichev, Sergey D. T1 - Infrared spectroscopy for clinical diagnosis of dental pulp vitality T2 - 37th International Conference on Infrared, Millimetre, and Terahertz Waves (IRMMW-THz 37), 23-28 September 2012 , Wollongong, Australia N2 - Transmission spectra of wet human teeth and dentin slices, together with blood of different flow rates were investigated over a wide spectral range, from visible to terahertz (THz) light. The results make it possible to find the optimum light frequency for an all-optical determination of pulpal blood flow and, consequently, for clinically diagnosis of tooth vitality. Y1 - 2012 U6 - https://doi.org/10.1109/IRMMW-THz.2012.6380091 PB - IEEE CY - Piscataway, NJ ER - TY - JOUR A1 - Hirmer, Marion A1 - Danilov, Sergey N. A1 - Giglberger, Stephan A1 - Putzger, Jürgen A1 - Niklas, Andreas A1 - Jäger, Andreas A1 - Hiller, Karl-Anton A1 - Schmalz, Gottfried A1 - Redlich, Britta A1 - Schulz, Irene A1 - Monkman, Gareth J. A1 - Ganichev, Sergey D. T1 - Spectroscopic Study of Human Teeth and Blood from Visible to Terahertz Frequencies for Clinical Diagnosis of Dental Pulp Vitality JF - Journal of Infrared, Millimeter, and Terahertz Waves N2 - Transmission spectra of wet human teeth and dentin slices, together with blood of different flow rates were investigated. The measurements carried out over a wide spectral range, from visible light down to terahertz radiation. The results make it possible to find the optimum light frequency for an all-optical determination of pulpal blood flow and, consequently, for clinically diagnosis of tooth vitality. KW - Infrared and terahertz transmission KW - Dentin KW - Lentic or flowing blood KW - Dental pulp vitality Y1 - 2012 U6 - https://doi.org/10.1007/s10762-012-9872-3 SN - 1866-6906 VL - 33 SP - 366 EP - 375 PB - Springer ER - TY - GEN A1 - Hirmer, Marion A1 - Danilov, Sergey N. A1 - Giglberger, Stephan A1 - Putzger, Jürgen A1 - Niklas, Andreas A1 - Jäger, Andreas A1 - Hiller, Karl-Anton A1 - Schmalz, Gottfried A1 - Redlich, Britta A1 - Monkman, Gareth J. A1 - Ganichev, Sergey D. T1 - Spectroscopic Study of Human Teeth and Blood from Visible to Terahertz Frequencies for Clinical Diagnosis of Dental Pulp Vitality T2 - PACS, November 11, 2011 N2 - Transmission spectra of wet human teeth and dentin slices, together with blood of different flow rates were investigated. The measurements carried out over a wide spectral range, from visible light down to terahertz radiation. The results make it possible to find the optimum light frequency for an all-optical determination of pulpal blood flow and, consequently, for clinically diagnosis of tooth vitality. Y1 - 2011 ER - TY - JOUR A1 - Knörzer, S. A1 - Hiller, Karl-Anton A1 - Brandt, M. A1 - Niklas, Andreas A1 - Putzger, Jürgen A1 - Monkman, Gareth J. A1 - Danilov, Sergey N. A1 - Ganichev, Sergey D. A1 - Schulz, Irene A1 - Schmalz, Gottfried T1 - Detection of pulsed blood flow through a molar pulp chamber and surrounding tissue in vitro JF - Journal of Clinical Oral Investigations N2 - OBJECTIVES Due to severe limitations of dental pulp sensitivity tests, the direct recording of pulsed blood flow, using photoplethysmography (PPG), has been proposed. In vivo evaluation is methodologically difficult and in vitro models have hitherto been adversely influenced by shortcomings in emulating the in vivo situation. Consequently, the aim of this study was to test an improved data acquisition system and to use this configuration for recording pulsed blood in a new model. MATERIALS AND METHODS We introduced a PPG signal detection system by recording signals under different blood flow conditions at two wavelengths (625 and 940 nm). Pulsed blood flow signals were measured using an in vitro model, containing a molar with a glass pulp and a resin socket, which closely resembled in vivo conditions with regard to volumetric blood flow, pulp anatomy, and surrounding tissue. RESULTS The detection system showed improved signal strength without stronger blanketing of noise. On the tooth surface, it was possible to detect signals emanating from pulsed blood flow from the glass pulp and from surrounding tissue at 625 nm. At 940 nm, pulp derived signals were recorded, without interference signals from surrounding tissue. CONCLUSION The PPG-based method has the potential to detect pulsed blood flow in small volumes in the pulp and (at 625 nm) also in adjacent tissues. CLINICAL RELEVANCE The results show the need for clear differentiation of the spatial origins of blood flow signals of any vitality test method to be applied to teeth. KW - Dental Pulp KW - Dental Pulp Cavity KW - Dental Pulp Test KW - Laser-Doppler Flowmetry KW - Molar Y1 - 2019 U6 - https://doi.org/10.1007/s00784-018-2530-y VL - 23 IS - 3 SP - 1121 EP - 1132 PB - Springer Nature ER - TY - JOUR A1 - Widbiller, Matthias A1 - Keim, Lukas A1 - Schlichting, Ralf A1 - Striegl, Birgit A1 - Hiller, Karl-Anton A1 - Jungbauer, Rebecca A1 - Buchalla, Wolfgang A1 - Galler, Kerstin M. T1 - Debris Removal by Activation of Endodontic Irrigants in Complex Root Canal Systems BT - A Standardized In-Vitro-Study JF - Applied Sciences N2 - Aim of the study was to develop a standardized model system to investigate endodontic irrigation techniques and assess the efficiency of different activation methods on the removal of hard tissue debris in complex root canal systems. Mesial roots of mandibular molars were firstly scanned by micro-computed tomography (µCT) and allocated to three groups of irrigant activation: sonic activation (EDDY, VDW, Munich, Germany), laser activation (AutoSWEEPS, FOTONA, Ljubljana, Slovenia) and conventional needle irrigation (control). Roots were fixed in individual 3D-printed holders to facilitate root canal enlargement under constant irrigation with NaOCl (5%). To enable standardized quantification of remaining debris, BaSO4-enriched dentine powder was compacted into the canals, followed by another µCT-scan. The final irrigation was performed using 17% ethylenediaminetetraacetic acid (EDTA) and 5% sodium hypochlorite (NaOCl) with the respective activation method, and the volume of remaining artificial debris was quantified after a final µCT-scan. The newly developed model system allowed for reliable, reproducible and standardized assessment of irrigation methods. Activation of the irrigant proved to be significantly more effective than conventional needle irrigation regarding the removal of debris, which persisted particularly in the apical third of the root canal in the control group. The efficiency of irrigation was significantly enhanced with laser- and sonic-based activation, especially in the apical third. Y1 - 2021 U6 - https://doi.org/10.3390/app11167331 VL - 11 IS - 16 SP - 1 EP - 14 PB - MDPI CY - Basel ER -