<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>130</id>
    <completedYear/>
    <publishedYear>2010</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>161</pageFirst>
    <pageLast>171</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>21</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Cerebral bio-imaging of Cu, Fe, Zn and Mn in the MPTP mouse model of Parkinsons disease using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)</title>
    <abstract language="eng">Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronically intoxicated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) as a model of Parkinson’s disease. Images were calibrated using matrix-matched laboratory standards. A software solution allowing a precise delineation of anatomical structures was implemented. Coronal brain sections were analyzed crossing the striatum and the substantia nigra, respectively. Animals sacrificed 2 h, 7 d, or 28 d after the last MPTP injection and controls were investigated.&#13;
We observed significant decreases of Cu concentrations in the periventricular zone and the fascia dentata at 2 h and 7d and a recovery or overcompensation at 28 d, most pronounced in the rostral periventricular zone (+40%). In the cortex Cu decreased slightly to −10%. Fe increased in the interpeduncular nucleus (+40%) but not in the substantia nigra. This pattern is in line with a differential regulation of periventricular and parenchymal Cu, and with the histochemical localization of Fe, and congruent to regions of preferential MPTP binding described in the rodent brain.&#13;
The LA-ICP-MS technique yielded valid and statistically robust results in the present study on 39 slices from 19 animals. Our findings underline the value of routine micro-local analytical techniques in the life sciences and affirm a role of Cu availability in Parkinson’s disease.</abstract>
    <parentTitle language="eng">Journal of the American Society for Mass Spectrometry</parentTitle>
    <identifier type="doi">10.1016/j.jasms.2009.09.022</identifier>
    <author>Andreas Matusch</author>
    <author>Candan Depboylu</author>
    <author>Christoph Palm</author>
    <author>Bei Wu</author>
    <author>Günter U. Höglinger</author>
    <author>Martin K.-H. Schäfer</author>
    <author>Johanna Sabine Becker</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Inductively Couple Plasma Mass Spectrometry</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Substantia Nigra</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>MPTP</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Laser Ablation Inductively Couple Plasma Mass Spectrometry</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>MPTP Treatment</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>swd</type>
      <value>ICP-Massenspektrometrie</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>swd</type>
      <value>Metalle</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>swd</type>
      <value>Gehirnkarte</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>swd</type>
      <value>MPTP</value>
    </subject>
    <collection role="ddc" number="0">Informatik, Informationswissenschaft, allgemeine Werke</collection>
    <collection role="institutes" number="FakIM">Fakultät Informatik und Mathematik</collection>
    <collection role="persons" number="palmremic">Palm, Christoph (Prof. Dr.) - ReMIC</collection>
    <collection role="othpublikationsherkunft" number="">Externe Publikationen</collection>
    <collection role="othforschungsschwerpunkt" number="16314">Lebenswissenschaften und Ethik</collection>
    <collection role="institutes" number="">Labor Regensburg Medical Image Computing (ReMIC)</collection>
  </doc>
</export-example>
