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  <doc>
    <id>6123</id>
    <completedYear/>
    <publishedYear>2023</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>21</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>10</volume>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes</title>
    <abstract language="eng">Osteoarthritis (OA) is a degenerative joint disease causing loss of articular cartilage and structural damage in all joint tissues. Given the limited regenerative capacity of articular cartilage, methods to support the native structural properties of articular cartilage are highly anticipated. The aim of this study was to infiltrate zwitterionic monomer solutions into human OA-cartilage explants to replace lost proteoglycans. The study included polymerization and deposition of methacryloyloxyethyl-phosphorylcholine- and a novel sulfobetaine-methacrylate-based monomer solution within ex vivo human OA-cartilage explants and the encapsulation of isolated chondrocytes within hydrogels and the corresponding effects on chondrocyte viability. The results demonstrated that zwitterionic cartilage–hydrogel networks are formed by infiltration. In general, cytotoxic effects of the monomer solutions were observed, as was a time-dependent infiltration behavior into the tissue accompanied by increasing cell death and penetration depth. The successful deposition of zwitterionic hydrogels within OA cartilage identifies the infiltration method as a potential future therapeutic option for the repair/replacement of OA-cartilage extracellular suprastructure. Due to the toxic effects of the monomer solutions, the focus should be on sealing the OA-cartilage surface, instead of complete infiltration. An alternative treatment option for focal cartilage defects could be the usage of monomer solutions, especially the novel generated sulfobetaine-methacrylate-based monomer solution, as bionic for cell-based 3D bioprintable hydrogels.</abstract>
    <parentTitle language="eng">Bioengineering</parentTitle>
    <identifier type="doi">10.3390/bioengineering10070767</identifier>
    <identifier type="urn">urn:nbn:de:bvb:898-opus4-61235</identifier>
    <note>Corresponding author: Helga Hornberger</note>
    <enrichment key="opus.import.data">@articlearticle, author = Köck, Hannah and Striegl, Birgit and Kraus, Annalena and Zborilova, Magdalena and Christiansen, Silke and Schäfer, Nicole and Grässel, Susanne and Hornberger, H., year = 2023, month = 06, pages = 767, title = In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes, volume = 10, journal = Bioengineering, doi = 10.3390/bioengineering10070767</enrichment>
    <enrichment key="opus.import.dataHash">md5:259fefd1488ba80f897befdbf68256b4</enrichment>
    <enrichment key="opus.import.date">2023-06-30T15:13:21+00:00</enrichment>
    <enrichment key="opus.import.file">/tmp/phpviUmRr</enrichment>
    <enrichment key="opus.import.format">bibtex</enrichment>
    <enrichment key="opus.import.id">649ef1117e8476.40764068</enrichment>
    <enrichment key="CorrespondingAuthor">Helga Hornberger</enrichment>
    <enrichment key="BegutachtungStatus">peer-reviewed</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Hannah Köck</author>
    <author>Birgit Striegl</author>
    <author>Annalena Kraus</author>
    <author>Magdalena Zborilova</author>
    <author>Silke H. Christiansen</author>
    <author>Nicole Schäfer</author>
    <author>Susanne Grässel</author>
    <author>Helga Hornberger</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>osteoarthritis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>human articular cartilage</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>chondrocytes</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>hydrogels</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>zwitterionic monomers</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>infiltration</value>
    </subject>
    <collection role="institutes" number="FAKMB">Fakultät Maschinenbau</collection>
    <collection role="institutes" number="RCBE">Regensburg Center of Biomedical Engineering - RCBE</collection>
    <collection role="oaweg" number="">Gold Open Access- Erstveröffentlichung in einem/als Open-Access-Medium</collection>
    <collection role="persons" number="hornbergerbma">Hornberger, Helga (Prof. Dr.) - Labor Biomaterialien</collection>
    <collection role="othforschungsschwerpunkt" number="16314">Lebenswissenschaften und Ethik</collection>
    <collection role="oaweg" number="">Corresponding author der OTH Regensburg</collection>
    <collection role="funding" number="">Publikationsfonds der OTH Regensburg</collection>
    <collection role="institutes" number="">Labor Biomaterialien</collection>
    <thesisPublisher>Ostbayerische Technische Hochschule Regensburg</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-oth-regensburg/files/6123/Hornberger_bioengineering-10-00767.pdf</file>
  </doc>
</export-example>
