@article{HornRaptisFischeretal., author = {Horn, Lars-Christian and Raptis, Georgios and Fischer, Uta and Hentschel, Bettina and K{\"o}hler, U. and Richter, Christine E. and Martin, Rosemarie}, title = {CD44-v6 concentrations in carcinoma of the uterine cervix: lack of prognostic significance}, series = {Archives of Gynecology and Obstetrics}, volume = {273}, journal = {Archives of Gynecology and Obstetrics}, number = {2}, doi = {10.1007/s00404-005-0039-5}, pages = {104 -- 106}, abstract = {Several kinds of cellular adhesion molecules, like different splicing variants of CD 44, have gained important as prognostic or markers for metastatic disease. Fresh frozen samples from 64 cervical carcinoma (CX) were stored in liquid nitrogen and examined using ELISA-technique, testing the prognostic impact. Normal cervical tissue served as control. CD 44-v6 concentration, was significant elevated in tumor tissue, when compared to the controls (P=0.04). There was no correlation to tumor stage (P=0.61), lymphovascular space involvement (P=0.075) or pelvic lymph node involvement (P=0.81). The CD 44-v6 concentration was not informative regarding recurrence-free and overall survival. Contrary to immunohistochemistry, the quantification of CD 44-v6 using ELISA-technique does not provide any further information.}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornRaptisNenning, author = {Horn, Lars-Christian and Raptis, Georgios and Nenning, Hans}, title = {DNA cytometric analysis of surgically treated squamous cell cancer of the uterine cervix, stage pT1b1-pT2b}, series = {Analytical and quantitative cytology and histology}, volume = {24}, journal = {Analytical and quantitative cytology and histology}, number = {1}, pages = {23 -- 29}, abstract = {Objective: To determine the utility of DNA content and DNA-related variables of proliferative activity regarding prognosis in cervical cancer. Study design: DNA image (ICM) andflow cytometry (FCM) were performed to determine the DNA index (DI), 5c-exceeding rate (5c-ER), S-phase fraction (SPF) and proliferation index (PI) in 163 patients with surgically staged pT1b1-pT2b squamous cell cancer of the uterine cervix and treated with primary radical hysterectomy. ICM was performed on imprint cytology, obtained from fresh tumor tissue, which was also used for FCM. Results were analyzed using the chi2 test and Cox regression analysis for risk of pelvic lymph node involvement, tumor recurrence and recurrence-free survival (RFS). Results: ICM was performed on all 163 and FCM on 133 samples. One-third of the tumors showed DNA aneuploidy. Analysis demonstrated prognostic significance of a DI > or = 1.70, with a (70:30) 2.3-fold risk of recurrence (P=.024) and reduced RFS of 10 months (P=.003) in cases of DI > or = 1.70. A high 5c-ER > 11\% was associated with pelvic lymph node involvement and decreased RFS (P < or = .04). Significantly more relapses were found in tumors with SPF > 12\% (70.8\% vs. 29.2\%, P=.007). RFS was markedly reduced in tumors with high SPF (52.3 vs. 61.1 months, P=.011). Low proliferative tumors (PI<25\%) were associated with lower stage (P=.036) and increased RFS (61.2 vs. 47.1 months, P=.028). In multivariate analysis of clinicopathologic variables (pT category, nodal status, lymphovascular space involvement) and DNA related variables, pelvic lymph node involvement was the only significant predictor of RFS. In patients with nodal involvement, tumors with DI >1.70 were associated with lessfavorable outcomes. Conclusion: DNA-related variables of cell cycle analysis were valuablefor predicting prognosis in cervical cancer patients. Tumors with DI>1.70, 5c-ER >11\% and high proliferative activity (SPF>12\%, PI>25\%) represent a subgroup with a poor prognosis.}, subject = {Geb{\"a}rmutterhals}, language = {en} } @inproceedings{HornPippigRaptisetal., author = {Horn, Lars-Christian and Pippig, S. and Raptis, Georgios and Fischer, Uta and K{\"o}hler, U. and Hentschel, Bettina and Martin, Rosemarie}, title = {Tumorassoziierte Proteolyse beim Zervixkarzinom}, series = {87. Tagung der Deutschen Gesellschaft f{\"u}r Pathologie, 14. Juni 2003}, booktitle = {87. Tagung der Deutschen Gesellschaft f{\"u}r Pathologie, 14. Juni 2003}, subject = {Geb{\"a}rmutterhalskrebs}, language = {de} } @inproceedings{HoehRaptis, author = {H{\"o}h, H. and Raptis, Georgios}, title = {Applications of the Erbium: YAG Laser in Ophthalmology - Cataracta-, Glaukoma-, Eyelid-Surgery and pars plana Vitrectomy}, abstract = {Recent developments in lasers have provided us the possibility of laser ocular surgery. The xenon, argon, neodymium:YAG and dye lasers have been successfully used in out-patient clinics. The excimer laser has been attracting researchers' interest in the new application of laser to cornea and lens. The erbium:YAG laser emits a 2.94-microns beam that can ablate the transparent ocular tissues such as lenses and corneas. The author has applied this laser to the cornea, lens, vitreous and other ocular tissues. The erbium:YAG laser beam was directed through a 1.5-meter-long, 200-microns-diameter fiberoptic guide. The radiant energy measured about 50 mJ at the end of the probe. The laser was emitted as a 400-microsecond pulse. Freshly enucleated rabbit eyes were used in this study. Laser burns were applied to the tissue surface at various energy settings. At minimal power, the tissues were coagulated by the erbium:YAG laser application. At a power of more than 636-954 mJ/mm2, tissue began to evaporate; the tissue loss was observed under a surgical light microscope. Corneal photoablation, lens ablation, iridotomy, trabeculotomy, cutting of the vitreous and retinal ablation were easily performed. Like the excimer laser, the erbium:YAG laser is a potential tool for ocular surgery.}, subject = {Augenchirurgie}, language = {en} } @article{ChaloupkaRaptisZachetal., author = {Chaloupka, I. and Raptis, Georgios and Zach, A. and Br{\"a}ndlin, K. and Meier-Ewert, H.}, title = {Rolle der quantitativen CMV-DNA-Bestimmungen in der Diagnostik und Therapie der Cytomegalovirusinfektion bei immunsupprimierten Patienten}, series = {Jahrestagung der Gesellschaft f{\"u}r Virologie in Wien, April 2000}, journal = {Jahrestagung der Gesellschaft f{\"u}r Virologie in Wien, April 2000}, subject = {Cytomegalie-Virus}, language = {de} } @article{HornPippigRaptisetal., author = {Horn, Lars-Christian and Pippig, S. and Raptis, Georgios and Fischer, Uta and K{\"o}hler, U. and Hentschel, Bettina and Martin, Rosemarie}, title = {Clinical relevance of urokinase-type plasminogen activator and its inhibitor type-1 (PAI-1) in squamous cell carcinoma of the uterine cervix}, series = {Australian and New Zealand Journal of Obstetrics and Gynaecology}, volume = {42}, journal = {Australian and New Zealand Journal of Obstetrics and Gynaecology}, number = {4}, doi = {10.1111/j.0004-8666.2002.00385.x}, pages = {385 -- 388}, abstract = {Objective The expression of uPA and PAI-1 as parameters of tumour-associated proteolysis has been implicated in the process of tumour cell invasion and the metastatic process. However, there is limited information on the impact of these parameters in cervical carcinoma. Methods Quantitative levels for uPA (n = 114) and PAI-1 (n = 103) were researched in operatively treated, surgically staged squamous cell cancer of the uterine cervix, using an ELISA-technique. Results were assessed regarding their impact in predicting pelvic lymph nodes metastases, tumour recurrence rate and recurrence free survival (RFS) using uni- and multivariate analysis. Results Median levels of both parameters were significantly higher in tumour tissue than in normal cervical tissue (p < 0.001). Detection of uPA gave no useful prognostic information. PAI-1 concentration showed a positive correlation with advanced tumour stage (p = 0.008), but no significant correlation with nodal status (pN0: 2.6 vs. pN1: 4.0 ng/mg protein; p = 0.092). Using a cut-off level of 2.4 ng/mg protein, patients with elevated PAI-1 levels demonstrated reduced RFS (45.9 versus 52.9 months; p = 0.1). Multivariate analysis, including nodal status, tumour stage, lymphovascular space involvement and grading failed to demonstrate any prognostic impact of uPA and PAI-1. Conclusions The results indicate, that PAI-1 expression is of some prognostic impact in cervical cancer, indicating an association of elevated PAI levels with local tumour progression and reduced recurrence-free survival.}, subject = {Plasminogen human-Aktivator}, language = {en} } @article{FischerRaptisGessneretal., author = {Fischer, Uta and Raptis, Georgios and Geßner, W. and Roschlau, U. and Dressel, K. and Bilek, Karin and Horn, Lars-Christian}, title = {Epidemiologie und formale Pathogenese des Zervixkarzinoms}, series = {Zentralblatt f{\"u}r Gyn{\"a}kologie}, volume = {123}, journal = {Zentralblatt f{\"u}r Gyn{\"a}kologie}, number = {4}, pages = {198 -- 205}, subject = {Geb{\"a}rmutterhalskrebs}, language = {de} } @article{HornFischerHaneletal., author = {Horn, Lars-Christian and Fischer, Uta and Hanel, C. and Kuhn, H. and Raptis, Georgios and Bilek, Karin}, title = {p53 in surgically treated and pathologically staged cervical cancer}, series = {Pathology - Research and Practice}, volume = {197}, journal = {Pathology - Research and Practice}, number = {9}, pages = {605 -- 609}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornRaptisFischeretal., author = {Horn, Lars-Christian and Raptis, Georgios and Fischer, Uta and Bilek, Karin}, title = {Impact of tumorload in surgically treated, histologically staged early cervical cancer: Multivariate analysis of 568 patients}, series = {International Journal of Gynecology \& Obstetrics}, volume = {70}, journal = {International Journal of Gynecology \& Obstetrics}, number = {Suppl. 1}, pages = {20 -- 21}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornRaptisFischeretal., author = {Horn, Lars-Christian and Raptis, Georgios and Fischer, Uta and Bilek, Karin}, title = {p53-tumorsuppressor gene in surgically treated histologically staged early cervical cancer}, series = {International Journal of Gynecology \& Obstetrics}, volume = {70}, journal = {International Journal of Gynecology \& Obstetrics}, number = {Suppl. 1}, pages = {112}, subject = {Tumorsuppressor-Gen}, language = {en} }