@inproceedings{PalmSchollLehmannetal., author = {Palm, Christoph and Scholl, Ingrid and Lehmann, Thomas M. and Spitzer, Klaus}, title = {Nutzung eines Farbkonstanz-Algorithmus zur Entfernung von Glanzlichtern in laryngoskopischen Bildern}, series = {Methoden der Medizinischen Informatik, Biometrie und Epidemiologie in der modernen Informationsgesellschaft}, booktitle = {Methoden der Medizinischen Informatik, Biometrie und Epidemiologie in der modernen Informationsgesellschaft}, editor = {Greiser, E. and Wischnewsky, M.}, publisher = {MMV Medien und Medizin}, address = {M{\"u}nchen}, isbn = {9783820813357}, pages = {300 -- 303}, abstract = {1 Einf{\"u}hrung Funktionelle und organische St{\"o}rungen im Larynx beeintr{\"a}chtigen die Ausdrucksf{\"a}higkeit des Menschen. Zur Diagnostik und Verlaufkontrolle werden die Stimmlippen im Larynx mit Hilfe der Video-Laryngoskopie aufgenommen. Zur optimalen Farbmessung wird dazu an das Lupenendoskop eine 3-Chip-CCD-Kamera angeschlossen, die eine unabh{\"a}ngige Aufnahme der drei Farbkan{\"a}le erlaubt. Die bisherige subjektive Befundung ist von der Erfahrung des Untersuchers abh{\"a}ngig und l{\"a}ßt nur eine grobe Klassifikation der Krankheitsbilder zu. Zur Objektivierung werden daher quantitative Parameter f{\"u}r Farbe, Textur und Schwingung entwickelt. Neben dem Einfluß der wechselnden Lichtquellenfarbe auf den Farbeindruck ist die Sekretauflage auf den Stimmlippen ein Problem bei der Farb-und Texturanalyse. Sie kann zu ausgedehnten Glanzlichtern f{\"u}hren und so weite Bereiche der Stimmlippen f{\"u}r die Farb-und Texturanalyse unbrauchbar machen. Dieser Beitrag stellt einen Farbkonstanz-Algorithmus vor, der unabh{\"a}ngig von der Lichtquelle quantitative Farbwerte des Gewebes liefert und die Glanzlichtdetektion und -elimination erm{\"o}glicht. 2 Methodik Ziel des Farbkonstanz-Algorithmus ist die Trennung von Lichtquellen-und Gewebefarbe. Unter Verwendung des dichromatischen Reflexionsmodells [1] kann die Oberfl{\"a}chenreflexion mit der Farbe der Lichtquelle und die K{\"o}rperreflexion mit der Gewebefarbe identifiziert werden. Der Farbeindruck entsteht aus der Linearkombination beider Farbkomponenten. Ihre Gewichtung ist von der Aufnahmegeometrie abh{\"a}ngig, insbesondere vom Winkel zwischen Oberfl{\"a}chennormalen und dem Positionsvektor der Lichtquelle. In einem zweistufigen Verfahren wird zun{\"a}chst die Lichtquellenfarbe gesch{\"a}tzt, dann die Gewebefarbe ermittelt. Hieraus k{\"o}nnen beide Farbanteile durch die Berechnung der Gewichtsfaktoren pixelweise getrennt werden.}, language = {de} } @misc{OPUS4-418, title = {Advances in Quantitative Laryngoscopy, Voice and Speech Research, Procs. 3rd International Workshop, RWTH Aachen}, editor = {Lehmann, Thomas M. and Palm, Christoph and Spitzer, Klaus and Tolxdorff, Thomas}, address = {Aachen}, language = {en} } @inproceedings{PalmPelkmannLehmannetal., author = {Palm, Christoph and Pelkmann, Annegret and Lehmann, Thomas M. and Spitzer, Klaus}, title = {Distortion Correction of Laryngoscopic Images}, series = {Advances in quantitative laryngoscopy, voice and speech research, Proceedings of the 3rd international workshop Aachen, RWTH}, booktitle = {Advances in quantitative laryngoscopy, voice and speech research, Proceedings of the 3rd international workshop Aachen, RWTH}, pages = {117 -- 125}, abstract = {Laryngoscopic images of the vocal tract are used for diagnostic purposes. Quantitative mea-surements like changes of the glottis size or the surface of the vocal cords during an image sequence can be helpful to describe the healing process or to compare the findings of diffe-rent patients. Typically the endoscopic images are circulary symmetric distorted (barrel di-stortion). Therefore measurements of geometric dimensions depend on the object´s position in the image. In this paper an algorithm is presented which allows the computation of the translational invariant "real" object size by correcting the image distortion without using additional calibration of the optical environment.}, language = {en} } @inproceedings{PalmNeuschaeferRubeLehmannetal., author = {Palm, Christoph and Neuschaefer-Rube, C. and Lehmann, Thomas M. and Spitzer, Klaus}, title = {Wissensbasierte Bewegungskompensation in aktiven Konturmodellen}, series = {Bildverarbeitung f{\"u}r die Medizin}, booktitle = {Bildverarbeitung f{\"u}r die Medizin}, editor = {Evers, H. and Glombitza, G. and Lehmann, Thomas M. and Meinzer, H.-P.}, publisher = {Springer}, address = {Berlin}, doi = {10.1007/978-3-642-60125-5_2}, pages = {8 -- 12}, abstract = {Zur Analyse von Lippenbewegungsabl{\"a}ufen wird ein aktives Konturmodell eingesetzt. Probleme bereitet die hohe Sprechgeschwindigkeit, die in star ken Objektverschiebungen result iert und bislang nicht durch eine alleinige Konturanpassung kompensiert werden kann. In diesem Beitrag werden die klassischen aktiven Konturmodelle um eine Vorjustierung der Grobkonturen erweitert, die eine energiebasierte Konturanpassung erst m{\"o}glich macht. Die Sch{\"a}tzung der Verschiebung zur Vorjustierung basiert auf dem Gradientenbild und einem pr{\"a}dikatenlogisch formulierten Regelwerk, das Annahmen und Nebenbedingungen als Wissensbasis enth{\"a}lt. Mit Hilfe dieser Erweiterungen ist eine automatisierte Konturverfolgung der Lippen m{\"o}glich.}, language = {de} } @inproceedings{PalmMetzlerMohametal., author = {Palm, Christoph and Metzler, V. and Moham, B. and Dieker, O. and Lehmann, Thomas M. and Spitzer, Klaus}, title = {Co-Occurrence Matrizen zur Texturklassifikation in Vektorbildern}, series = {Bildverarbeitung f{\"u}r die Medizin}, booktitle = {Bildverarbeitung f{\"u}r die Medizin}, editor = {Evers, H. and Glombitza, G. and Lehmann, Thomas M. and Meinzer, H.-P.}, publisher = {Springer}, address = {Berlin}, doi = {10.1007/978-3-642-60125-5_69}, pages = {367 -- 371}, abstract = {Statistische Eigenschaften nat{\"u}rlicher Grauwerttexturen werden mit Co-Occurrence Matrizen, basierend auf der Grauwertstatistik zweiter Ordnung, modelliert. Die Matrix gibt dann die apriori Wahrscheinlichkeiten aller Grauwertpaare an. Da in der medizinischen Bildverarbeitung verst{\"a}rkt Multispektralbilder ausgewertet werden, wird das bekannte Konzept hier auf beliebige Vektorbilder erweitert. Dadurch kann bei der Texturklassifikation die zur Verf{\"u}gung stehende Information vollst{\"a}ndig genutzt werden. Insbesondere zur Detektion von Farbtexturen ist dieser Ansatz geeignet, da Wertepaare unterschiedlicher Spektralebenen ausgewertet werden k{\"o}nnen. Ebenso kann die Methode auch bei der Multiskalendekomposition von Intensit{\"a}tsbildern zur Verbesserung der Texturerkennung beitragen. Die in den Matrizen entstehenden Muster lassen dann {\"u}ber die Extraktion geeigneter Texturdeskriptoren R{\"u}ckschl{\"u}sse auf die Texturen des Bildes zu.}, language = {de} } @inproceedings{PalmLehmannSpitzer, author = {Palm, Christoph and Lehmann, Thomas M. and Spitzer, Klaus}, title = {Bestimmung der Lichtquellenfarbe bei der Endoskopie mikrotexturierter Oberfl{\"a}chen des Kehlkopfes}, series = {5. Workshop Farbbildverarbeitung, Ilmenau, 1999}, booktitle = {5. Workshop Farbbildverarbeitung, Ilmenau, 1999}, pages = {3 -- 10}, abstract = {Zur Unterst{\"u}tzung der Diagnose von Stimmlippenerkrankungen werden innerhalb des Forschungsprojektes Quantitative Digitale Laryngoskopie objektive Parameter zur Beschreibung der Bewegung, der Farbe sowie der Form der Stimmlippen entwickelt und klinisch evaluiert. W{\"a}hrend die Bewegungsanalyse Aufschluß {\"u}ber funktionelle Stimmst{\"o}rungen gibt, beschreiben Parameter der Farb- und Formanalyse morphologische Ver{\"a}nderungen des Stimmlippengewebes. In diesem Beitrag werden die Methoden und bisherigen Ergebnisse zur Bewegungs- und Farbanalyse vorgestellt. Die Bewegungsanalyse wurde mit einem erweiterten Konturmodell (Snakes) durchgef{\"u}hrt. Aufgrund des modifizierten Konturmodells konnten die Konturen der Stimmlippen automatisch {\"u}ber die gesmate Bildsequenz zuverl{\"a}ssig detektiert werden. Die Vermssung der Konturen liefert neue quantitative Parameter zur Befundung von laryngoskopischen Stimmlippenaufnahmen. Um die Farbeigenschaften der Stimmlippen zu bestimmen, wurde ausgehend vom RGB-Bild die Objektfarbe unabh{\"a}ngig von der Farbe der Lichtquelle durch Verwendung von Clusterverfahren und der Viertelkreisanalyse berechnet. Mit dieser Farbanalyse konnte die Farbe der Lichtquelle ermittelt und das beleuchtungsunabh{\"a}ngige Farbbild berechnet werden. Die Quanitifizierung der R{\"o}tung der Stimmlippen ist z.B. ein entscheidendes Kriterium zur Diagnostik der akuten Laryngitis.}, language = {de} } @article{HartmannWeihererSchiltzetal., author = {Hartmann, Robin and Weiherer, Maximilian and Schiltz, Daniel and Seitz, Stephan and Lotter, Luisa and Anker, Alexandra and Palm, Christoph and Prantl, Lukas and Br{\´e}bant, Vanessa}, title = {A Novel Method of Outcome Assessment in Breast Reconstruction Surgery: Comparison of Autologous and Alloplastic Techniques Using Three-Dimensional Surface Imaging}, series = {Aesthetic Plastic Surgery}, volume = {44}, journal = {Aesthetic Plastic Surgery}, publisher = {Springer}, address = {Heidelberg}, doi = {10.1007/s00266-020-01749-4}, pages = {1980 -- 1987}, abstract = {Background Breast reconstruction is an important coping tool for patients undergoing a mastectomy. There are numerous surgical techniques in breast reconstruction surgery (BRS). Regardless of the technique used, creating a symmetric outcome is crucial for patients and plastic surgeons. Three-dimensional surface imaging enables surgeons and patients to assess the outcome's symmetry in BRS. To discriminate between autologous and alloplastic techniques, we analyzed both techniques using objective optical computerized symmetry analysis. Software was developed that enables clinicians to assess optical breast symmetry using three-dimensional surface imaging. Methods Twenty-seven patients who had undergone autologous (n = 12) or alloplastic (n = 15) BRS received three-dimensional surface imaging. Anthropomorphic data were collected digitally using semiautomatic measurements and automatic measurements. Automatic measurements were taken using the newly developed software. To quantify symmetry, a Symmetry Index is proposed. Results Statistical analysis revealed that there is no dif- ference in the outcome symmetry between the two groups (t test for independent samples; p = 0.48, two-tailed). Conclusion This study's findings provide a foundation for qualitative symmetry assessment in BRS using automatized digital anthropometry. In the present trial, no difference in the outcomes' optical symmetry was detected between autologous and alloplastic approaches.}, subject = {Mammoplastik}, language = {en} } @inproceedings{PalmKeysersLehmannetal., author = {Palm, Christoph and Keysers, Daniel and Lehmann, Thomas M. and Spitzer, Klaus}, title = {Gabor Filtering of Complex Hue/Saturation Images for Color Texture Classification}, series = {Proceedings of the 5th Joint Conference on Information Science (JCIS) 2, The Association for Intelligent Machinery, Atlantic City, NJ, 2000}, booktitle = {Proceedings of the 5th Joint Conference on Information Science (JCIS) 2, The Association for Intelligent Machinery, Atlantic City, NJ, 2000}, pages = {45 -- 49}, abstract = {Objective: Complex hue/saturation images as a new approach for color texture classification using Gabor filters are introduced and compared with common techniques. Method: The interpretation of hue and saturationas polar coordinates allows direct use of the HSV-colorspace for Fourier transform. This technique is applied for Gabor feature extraction of color textures. In contrast to other color features based on the RGB-colorspace [1] the combination of color bands is done previous to the filtering. Results: The performance of the new HS-featuresis compared with that of RGB based as well as grayscale Gabor features by evaluating the classifi-cation of 30 natural textures. The new HS-featuresshow same results like the best RGB features but allow a more compact representation. On the averagethe color features improve the results of grayscale features. Conclusion: The consideration of the color information enhances the classification of color texture. The choice of colorspace cannot be adjudged finally, but the introduced features suggest the use of the HSV-colorspace with less features than RGB.}, language = {en} } @article{HornFischerRaptisetal., author = {Horn, Lars-Christian and Fischer, Uta and Raptis, Georgios and Bilek, Karin and Hentschel, Bettina}, title = {Tumor size is of prognostic value in surgically treated FIGO stage II cervical cancer}, series = {Gynecologic Oncology}, volume = {107}, journal = {Gynecologic Oncology}, number = {2}, doi = {10.1016/j.ygyno.2007.06.026}, pages = {310 -- 315}, abstract = {Objectives Tumor size is a well recognized prognostic factor in early stage cervical carcinoma (CX). However, limited knowledge exists about the value of tumor size in surgically treated CX with extrauterine extension. Methods 245 cases of local advanced CX (FIGO stage IIA and IIB) who received upfront surgery were evaluated regarding tumor size, regarding the prediction of pelvic lymph node involvement and recurrence free and overall survival during a median follow-up time of 54 months (95\% CI 45.4-62.6 months). Tumors larger than 4 cm were defined as bulky stage disease. Results Bulky disease was seen in 46.1\% (113/245). 60.2\% of these patients showed pelvic lymph node involvement, compared to 42.4\% (56/132) in non-bulky tumors (p = 0.006; odds ratio: 2.2 [95\% CI: 1.3-3.6]). Patients with bulky tumors showed an increase of recurrent disease (40.2\% vs. 28.0\%; p = 0.045). The relative risk for recurrent disease was 1.97 (95\% CI: 1.3-3.0). The 5-year overall survival rate was significantly lower (67.7\% [95\% CI: 58.2-74.8] vs. 49.5\% [95\% CI: 36.8-59.1]; p = 0.0015). In multivariate analysis, tumor stage, pelvic lymph node involvement and maximal tumor size were independent prognostic factors. Conclusions The results suggest that tumor size, defining bulky disease as tumors larger than 4 cm, is of prognostic impact also in FIGO stage II cervical carcinomas. A revised FIGO/TNM classification system similar to the subgrouping of stage IB CX is recommended for stage II using a cut-off value of 4 cm as discriminator: stage IIA1 and stage IIB1 for tumors with ≤ 4 cm and IIA2 and IIB2 for tumors > 4 cm (i.e. bulky disease).}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornFischerRaptisetal., author = {Horn, Lars-Christian and Fischer, Uta and Raptis, Georgios and Bilek, Karin and Hentschel, Bettina and Richter, Christine E. and Braumann, Ulf Dietrich and Einenkel, Jens}, title = {Pattern of invasion is of prognostic value in surgically treated cervical cancer patients}, series = {Gynecologic Oncology}, volume = {103}, journal = {Gynecologic Oncology}, number = {3}, doi = {10.1016/j.ygyno.2006.05.046}, pages = {906 -- 911}, abstract = {Objectives: Different patterns of invasion (representing different grades of tumor cell dissociation) are associated with prognostic outcome in cancer. We evaluated the prognostic value of different patterns of invasion (PI) in cervical carcinomas (CX). Methods: Six hundred eleven surgically treated CX (FIGO IB to IIB) were re-evaluated histologically regarding the PI, using a three-level scoring system. Closed PI was defined as cohesive growth with well-delineated (pushing) borders. In finger-like PI the tumor grows in solid cords/trabecles. Highly dissociative growth in small groups or single cells was defined as spray-like PI. Types of PI were correlated to tumor stage, histo-morphologic factors and prognostic outcome. Results: Sixty percent of the tumors showed a spray-like PI, 30\% a finger-like PI and only 7.4\% were of the closed type. Spray-like PI showed a significant correlation with advanced stage disease, lymphovascular space involvement, poorly differentiated tumors and pelvic lymph node metastases. Spray-like PI was accompanied by a reduced 5-year overall survival when compared to the finger-like and closed PI (68.7\% vs. 80.9\% vs. 88.5\%; P=0.0004). The prognostic impact of the PI disappeared in node-positive patients (P=0.06) but persisted in patients without pelvic lymph node disease (P=0.03). In multivariate analysis, using COX regression model, the PI represented as independent prognostic factor. Conclusions: Spray-like PI (i.e., highest degree of tumor cell dissociation) is associated with advanced tumor stages, increased rate of recurrency and a reduced overall survival. In separate analysis of patients with and without lymph node metastases, the impact of PI persisted only in node-negative cases as a prognostic factor.}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornRaptisFischeretal., author = {Horn, Lars-Christian and Raptis, Georgios and Fischer, Uta and Hentschel, Bettina and K{\"o}hler, U. and Richter, Christine E. and Martin, Rosemarie}, title = {CD44-v6 concentrations in carcinoma of the uterine cervix: lack of prognostic significance}, series = {Archives of Gynecology and Obstetrics}, volume = {273}, journal = {Archives of Gynecology and Obstetrics}, number = {2}, doi = {10.1007/s00404-005-0039-5}, pages = {104 -- 106}, abstract = {Several kinds of cellular adhesion molecules, like different splicing variants of CD 44, have gained important as prognostic or markers for metastatic disease. Fresh frozen samples from 64 cervical carcinoma (CX) were stored in liquid nitrogen and examined using ELISA-technique, testing the prognostic impact. Normal cervical tissue served as control. CD 44-v6 concentration, was significant elevated in tumor tissue, when compared to the controls (P=0.04). There was no correlation to tumor stage (P=0.61), lymphovascular space involvement (P=0.075) or pelvic lymph node involvement (P=0.81). The CD 44-v6 concentration was not informative regarding recurrence-free and overall survival. Contrary to immunohistochemistry, the quantification of CD 44-v6 using ELISA-technique does not provide any further information.}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornRaptisNenning, author = {Horn, Lars-Christian and Raptis, Georgios and Nenning, Hans}, title = {DNA cytometric analysis of surgically treated squamous cell cancer of the uterine cervix, stage pT1b1-pT2b}, series = {Analytical and quantitative cytology and histology}, volume = {24}, journal = {Analytical and quantitative cytology and histology}, number = {1}, pages = {23 -- 29}, abstract = {Objective: To determine the utility of DNA content and DNA-related variables of proliferative activity regarding prognosis in cervical cancer. Study design: DNA image (ICM) andflow cytometry (FCM) were performed to determine the DNA index (DI), 5c-exceeding rate (5c-ER), S-phase fraction (SPF) and proliferation index (PI) in 163 patients with surgically staged pT1b1-pT2b squamous cell cancer of the uterine cervix and treated with primary radical hysterectomy. ICM was performed on imprint cytology, obtained from fresh tumor tissue, which was also used for FCM. Results were analyzed using the chi2 test and Cox regression analysis for risk of pelvic lymph node involvement, tumor recurrence and recurrence-free survival (RFS). Results: ICM was performed on all 163 and FCM on 133 samples. One-third of the tumors showed DNA aneuploidy. Analysis demonstrated prognostic significance of a DI > or = 1.70, with a (70:30) 2.3-fold risk of recurrence (P=.024) and reduced RFS of 10 months (P=.003) in cases of DI > or = 1.70. A high 5c-ER > 11\% was associated with pelvic lymph node involvement and decreased RFS (P < or = .04). Significantly more relapses were found in tumors with SPF > 12\% (70.8\% vs. 29.2\%, P=.007). RFS was markedly reduced in tumors with high SPF (52.3 vs. 61.1 months, P=.011). Low proliferative tumors (PI<25\%) were associated with lower stage (P=.036) and increased RFS (61.2 vs. 47.1 months, P=.028). In multivariate analysis of clinicopathologic variables (pT category, nodal status, lymphovascular space involvement) and DNA related variables, pelvic lymph node involvement was the only significant predictor of RFS. In patients with nodal involvement, tumors with DI >1.70 were associated with lessfavorable outcomes. Conclusion: DNA-related variables of cell cycle analysis were valuablefor predicting prognosis in cervical cancer patients. Tumors with DI>1.70, 5c-ER >11\% and high proliferative activity (SPF>12\%, PI>25\%) represent a subgroup with a poor prognosis.}, subject = {Geb{\"a}rmutterhals}, language = {en} } @inproceedings{HornPippigRaptisetal., author = {Horn, Lars-Christian and Pippig, S. and Raptis, Georgios and Fischer, Uta and K{\"o}hler, U. and Hentschel, Bettina and Martin, Rosemarie}, title = {Tumorassoziierte Proteolyse beim Zervixkarzinom}, series = {87. Tagung der Deutschen Gesellschaft f{\"u}r Pathologie, 14. Juni 2003}, booktitle = {87. Tagung der Deutschen Gesellschaft f{\"u}r Pathologie, 14. Juni 2003}, subject = {Geb{\"a}rmutterhalskrebs}, language = {de} } @inproceedings{HoehRaptis, author = {H{\"o}h, H. and Raptis, Georgios}, title = {Applications of the Erbium: YAG Laser in Ophthalmology - Cataracta-, Glaukoma-, Eyelid-Surgery and pars plana Vitrectomy}, abstract = {Recent developments in lasers have provided us the possibility of laser ocular surgery. The xenon, argon, neodymium:YAG and dye lasers have been successfully used in out-patient clinics. The excimer laser has been attracting researchers' interest in the new application of laser to cornea and lens. The erbium:YAG laser emits a 2.94-microns beam that can ablate the transparent ocular tissues such as lenses and corneas. The author has applied this laser to the cornea, lens, vitreous and other ocular tissues. The erbium:YAG laser beam was directed through a 1.5-meter-long, 200-microns-diameter fiberoptic guide. The radiant energy measured about 50 mJ at the end of the probe. The laser was emitted as a 400-microsecond pulse. Freshly enucleated rabbit eyes were used in this study. Laser burns were applied to the tissue surface at various energy settings. At minimal power, the tissues were coagulated by the erbium:YAG laser application. At a power of more than 636-954 mJ/mm2, tissue began to evaporate; the tissue loss was observed under a surgical light microscope. Corneal photoablation, lens ablation, iridotomy, trabeculotomy, cutting of the vitreous and retinal ablation were easily performed. Like the excimer laser, the erbium:YAG laser is a potential tool for ocular surgery.}, subject = {Augenchirurgie}, language = {en} } @article{ChaloupkaRaptisZachetal., author = {Chaloupka, I. and Raptis, Georgios and Zach, A. and Br{\"a}ndlin, K. and Meier-Ewert, H.}, title = {Rolle der quantitativen CMV-DNA-Bestimmungen in der Diagnostik und Therapie der Cytomegalovirusinfektion bei immunsupprimierten Patienten}, series = {Jahrestagung der Gesellschaft f{\"u}r Virologie in Wien, April 2000}, journal = {Jahrestagung der Gesellschaft f{\"u}r Virologie in Wien, April 2000}, subject = {Cytomegalie-Virus}, language = {de} } @article{HornPippigRaptisetal., author = {Horn, Lars-Christian and Pippig, S. and Raptis, Georgios and Fischer, Uta and K{\"o}hler, U. and Hentschel, Bettina and Martin, Rosemarie}, title = {Clinical relevance of urokinase-type plasminogen activator and its inhibitor type-1 (PAI-1) in squamous cell carcinoma of the uterine cervix}, series = {Australian and New Zealand Journal of Obstetrics and Gynaecology}, volume = {42}, journal = {Australian and New Zealand Journal of Obstetrics and Gynaecology}, number = {4}, doi = {10.1111/j.0004-8666.2002.00385.x}, pages = {385 -- 388}, abstract = {Objective The expression of uPA and PAI-1 as parameters of tumour-associated proteolysis has been implicated in the process of tumour cell invasion and the metastatic process. However, there is limited information on the impact of these parameters in cervical carcinoma. Methods Quantitative levels for uPA (n = 114) and PAI-1 (n = 103) were researched in operatively treated, surgically staged squamous cell cancer of the uterine cervix, using an ELISA-technique. Results were assessed regarding their impact in predicting pelvic lymph nodes metastases, tumour recurrence rate and recurrence free survival (RFS) using uni- and multivariate analysis. Results Median levels of both parameters were significantly higher in tumour tissue than in normal cervical tissue (p < 0.001). Detection of uPA gave no useful prognostic information. PAI-1 concentration showed a positive correlation with advanced tumour stage (p = 0.008), but no significant correlation with nodal status (pN0: 2.6 vs. pN1: 4.0 ng/mg protein; p = 0.092). Using a cut-off level of 2.4 ng/mg protein, patients with elevated PAI-1 levels demonstrated reduced RFS (45.9 versus 52.9 months; p = 0.1). Multivariate analysis, including nodal status, tumour stage, lymphovascular space involvement and grading failed to demonstrate any prognostic impact of uPA and PAI-1. Conclusions The results indicate, that PAI-1 expression is of some prognostic impact in cervical cancer, indicating an association of elevated PAI levels with local tumour progression and reduced recurrence-free survival.}, subject = {Plasminogen human-Aktivator}, language = {en} } @article{FischerRaptisGessneretal., author = {Fischer, Uta and Raptis, Georgios and Geßner, W. and Roschlau, U. and Dressel, K. and Bilek, Karin and Horn, Lars-Christian}, title = {Epidemiologie und formale Pathogenese des Zervixkarzinoms}, series = {Zentralblatt f{\"u}r Gyn{\"a}kologie}, volume = {123}, journal = {Zentralblatt f{\"u}r Gyn{\"a}kologie}, number = {4}, pages = {198 -- 205}, subject = {Geb{\"a}rmutterhalskrebs}, language = {de} } @article{HornFischerHaneletal., author = {Horn, Lars-Christian and Fischer, Uta and Hanel, C. and Kuhn, H. and Raptis, Georgios and Bilek, Karin}, title = {p53 in surgically treated and pathologically staged cervical cancer}, series = {Pathology - Research and Practice}, volume = {197}, journal = {Pathology - Research and Practice}, number = {9}, pages = {605 -- 609}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornRaptisFischeretal., author = {Horn, Lars-Christian and Raptis, Georgios and Fischer, Uta and Bilek, Karin}, title = {Impact of tumorload in surgically treated, histologically staged early cervical cancer: Multivariate analysis of 568 patients}, series = {International Journal of Gynecology \& Obstetrics}, volume = {70}, journal = {International Journal of Gynecology \& Obstetrics}, number = {Suppl. 1}, pages = {20 -- 21}, subject = {Geb{\"a}rmutterhalskrebs}, language = {en} } @article{HornRaptisFischeretal., author = {Horn, Lars-Christian and Raptis, Georgios and Fischer, Uta and Bilek, Karin}, title = {p53-tumorsuppressor gene in surgically treated histologically staged early cervical cancer}, series = {International Journal of Gynecology \& Obstetrics}, volume = {70}, journal = {International Journal of Gynecology \& Obstetrics}, number = {Suppl. 1}, pages = {112}, subject = {Tumorsuppressor-Gen}, language = {en} } @article{HornRaptisMartin, author = {Horn, Lars-Christian and Raptis, Georgios and Martin, Rosemarie}, title = {Tumorassoziierte Proteolyse, p53-Immunhistochemie und Zelladh{\"a}sion bei prim{\"a}r operierten Zervixkarzinomen}, series = {Geburtshilfe und Frauenheilkunde}, volume = {60}, journal = {Geburtshilfe und Frauenheilkunde}, number = {Suppl. 1}, pages = {200}, subject = {Tumorassoziierte Proteolyse}, language = {de} }