TY - INPR A1 - Kaul, Anja A1 - Böllmann, Andrea A1 - Thema, Martin A1 - Kalb, Larissa A1 - Stöckl, Richard A1 - Huber, Harald A1 - Sterner, Michael A1 - Bellack, Annett T1 - Identification of Robust Thermophilic Methanogenic Archaea and Packing Material for High Liquid Hold-Up at Low Volumetric Gas Flow Rates for Use in Trickle-Bed Reactors for Biological Methanation T2 - SSRN Electronic Journal N2 - he hydrogen gas-to-liquid mass transfer is the limiting factor in biological methanation. In a trickle-bed reactor, mass transfer can be increased by high flow velocities in the liquid phase, by adding a packing material with high liquid hold-up, or by choosing methanogenic archaea with a high methane productivity. This study analyzed various packings and methanogenic strains from a hydrodynamic and microbial perspective. By analyzing twelve pure cultures of thermophilic methanogens for their ability to produce high quality methane and to form biofilms on different packings, strains of Methanothermobacter were found to perform better than thus of the genus Methanothermococcus. Best methane production and adherence was observed on DuraTop®, Bioflow 9, and filter foam. DuraTop® and Bioflow 9 had also a high dynamic liquid hold-up, but the maximum hold-up was determined for expanded clay. The ideal combination for use in the ORBIT-trickle-bed reactor was identified as Methanothermobacter thermoautotrophicus IM5 and DuraTop®. KW - Liquid hold-up maximization KW - Methane production KW - Methanothermobacter KW - Methanothermococcus KW - Optimized packing-organism combination Y1 - 2021 UR - https://opus4.kobv.de/opus4-oth-regensburg/frontdoor/index/index/docId/2650 N1 - final peer reviewed article published under: https://doi.org/10.1016/j.biortech.2021.126524 ER -