- Rayleigh scattering in graded-index polymer optical fibers (GI-POF) is studied under the assumption that spherical scatterers are uniformly distributed within the layers of concentric ring structure. This approximation allows to describe typical imperfections occurring during the fabrication of GI-POFs. For the purpose of experimental characterization by side illumination, an analytical model of the light conversion into waveguide modes has been developed. This model describes the mode excitation by a multiplicative contribution of three different physical mechanisms. The corresponding experimental analysis of the light scattering has been performed and compared with numerical simulation results for the fibers with single-ring and multi-ring structures. Besides the application to GI-POFs, this article can be also applied to multi-step index fibers.