TY - JOUR A1 - Mueller, Silke A1 - März, Reinhard A1 - Schmolz, Manfred A1 - Drewelow, Bernd T1 - Intraindividual long term stability and response corridors of cytokines in healthy volunteers detected by a standardized whole-blood culture system for bed-side-application JF - BMC Medical Research Methodology N2 - Up to now, cytokines have been regarded as difficult parameters for the characterisation of the immune system. The study shows that, depending of the type of cytokine, the variability of these potent mediators of immune functions in fact be high high between subjects, but relatively small within a subject. By using appropriate statistical techniques, the use of cytokines as endpoints in investigational clinical studies has become possible. N2 - Bislang wurden Zytokine als schwierige Substanzen betrachtet, um Wirkungen auf das Immunsystem zu untersuchen. Die Studie zeigt, dass, je nach Art des Zytokins, die Unterschiede zwischen den Individuen zwar sehr groß sein können, die Variation innerhalb des Individuums jedoch etimmten Response-Korridors bleiben. Mit geeigneten statistischen Techniken kann diesem Umstand Rechnung getragen werden, so dass die Zytokine als klinische Endpunkte in klinischen Forschungsprojekten genutzt werden können. KW - Immunsystem KW - Cytokine KW - Tagesrhythmus KW - Diagnose KW - Immunology KW - cytokines KW - circadian rhythm KW - diagnostics Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:92-opus-1014 VL - 2012 PB - BioMed Central ER - TY - INPR A1 - Müller, Silke A1 - März, Reinhard A1 - Schmolz, Manfred A1 - Drewelow, Bernd A1 - Eschmann, Klaus A1 - Meiser, Peter T1 - Placebo-controlled randomized clinical trial on the immunomodulating activities of low- and high-dose bromelain after oral administration - new evidence on the antiinflammatory mode of action of bromelain N2 - In a randomized controlled multiple cross-over trial, the effect of a single application of 1000 and 3000 F.I.P. bromelain on 6 cytokines, of male healthy volunteers was studied. The analysis of cytokines (Interferon gamma, IL-2, IL-6, IL8, IL-10, IL13 and MCP-1) was performed with the TrueCulture(tm) whole-blood culture system (EDI GmbH). With a single dose of 3000 F. I. P. bromelain, IFNgamma was found to be significantly stimulated in the afternoon hours compared to placebo (p = 0.043; Mixed Linear Model, Bonferroni adjusted). N2 - In einer randomisierten, controlleirten Cross-Over-Studie wurde der Effekt einer einmaligen Appliaktion von 1000 bzw. 3000 F. I. P. Bromelain (Ursapharm GmbH, Saarbrücken)untersucht. Endpunkte waren 6 Zytokine(Interferon gamma, IL-2, IL-6, IL8, IL-10, IL13 and MCP-1), die mittels TrueCulture-Test-System (EDI-GmbH, Reutlingen) analysiert wurden. Die Analyse mittels Gemischtem Linearen Model ergab, dass die Doseriung von 3000 F.I.P. Bromelain einen signifikanten Anstieg im Vergleich zu Plazebo bewirkte (p=0,043, Bonferroni-korrigiert). KW - Immune System KW - bromelain KW - cytokines KW - antiinflammatory activity KW - circadian rhythm Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:92-opus-1022 N1 - Originalversion zu finden in: Phytotherpy Research. 27(2013)2, S.199-204 http://onlinelibrary.wiley.com/doi/10.1002/ptr.4678/abstract ER - TY - JOUR A1 - März, Reinhard A1 - Straußberger, Jürgen T1 - Demenz - das schleichende Vergessen T1 - Dementia - stepping into oblivion JF - Sozialmagazin N2 - Die Zahl an dementiell erkrankten Menschen steigt, eine wirksame Therapie ist nicht in Sicht. Aus der Erforschung der Risikofaktoren ist abzuleiten, dass motorische, kognitive und soziale Programme für die Prävention eine elementare Rolle spielen. Sie müssen allerdings möglichst frühzeitig begonnen werden, und zwar zu einem Zeitpunkt, zu dem die medizinische Diagnostik nur eine sog. leichte kognitive Beeinträchtigung feststellt, die für sich genommen noch gar keinen Krankheitswert hat, deren Übergangsrate zur Alzheimer-Demenz jedoch ca. 10 % beträgt. N2 - While more and more people suffer from dementia, but a potent treatment is not available up to now. The investigation of the risk factors has shown that physical, cognitive and social activities play an eminent role for the prevention. The must be initiated, before any clinical signs of dementia can be detected, in a stadium called mild cognitive impairment (MCI). MCI for itself has no clinical impact, but the conversion rate is about 10 %. KW - Alzheimer-Krankheit KW - Senile Demenz KW - Familienpflege KW - Psychosoziale Diagnostik KW - Betriebliche Sozialarbeit KW - Altenarbeit KW - Praevention KW - Risikofaktoren KW - Leichte kognitive Beeinträchtigung KW - LKB KW - risk factors KW - mild cognitive impairment KW - MCI Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:92-opus-1001 VL - 2010 IS - 12 ER - TY - JOUR A1 - Schmolz, Manfred A1 - März, Reinhard A1 - Schaudt, Marco A1 - Schaudt, Cornelia A1 - Lauster, Caroline T1 - Immunomodulatory Activities of a Concentrated Fruit and Vegetable Juice Tested in a Randomized, Placebo-Controlled, Double-Blind Clinical Trial in Healthy Volunteers JF - Food and Nutrition Sciences N2 - A randomized, placebo-controlled trial with healthy volunteers (n = 22) was conducted to investigate the immunomodulatory activities of a concentrated fruit and vegetable juice over a period of 7 weeks. Immune response was characterized with the True-culture (R) whole blood assay. Out of 30 cytokines significant increases (t-test, p< 0,05) were shown for the T-cell dependent cytokines GM-CSF, IFNgamma (p = 0,078), IL-4, IL-10, IL-17 and TNFbeta after 3 days of juice intake. The study confirmed former results of stable levels of cytokines over several weeks within the individual, while the variabilty between individuals can be high. Studies must be designed analyzed carefully to be able to characterize immune responses properly. KW - Klinisches Experiment KW - Cytokine KW - Saft KW - Randomized Placebo-Controlled Clinical Trial KW - Ex Vivo KW - Cytokine KW - Juice Concentrate Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:92-opus-1255 VL - 2014 IS - 5 ER -