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Studying dynamic aroma release by headspace-solid phase microextraction-gas chromatography-ion mobility spectrometry (HS-SPME-GC-IMS): method optimization, validation, and application

  • To understand aroma perception from complex food matrices‚ determination of dynamic aroma release during simulated oral processing is necessary. In this study optimization, validation and application of a novel method coupling headspace-solid phase microextraction (HS-SPME) with gas chromatography-ion mobility spectrometry (GC-IMS) is presented. Thirteen character impact compounds imparting different chemical properties are studied to understand capabilities and limitations of the method. It was shown for the first time that the temperature of the IMS sample inlet can be increased up to 200 °C without instrumental constraints. Linear calibration was possible for eleven of the thirteen compounds with one decade dynamic range. The limit of detection and quantitation were 2.1–63.0 ppb and 7.2–210.1 ppb, respectively. Diacetyl could be detected in negative polarity mode of IMS, however with lower precision compared to the compounds detected in positive mode. Limitations of the method were short HS-SPME extraction time, which in the caseTo understand aroma perception from complex food matrices‚ determination of dynamic aroma release during simulated oral processing is necessary. In this study optimization, validation and application of a novel method coupling headspace-solid phase microextraction (HS-SPME) with gas chromatography-ion mobility spectrometry (GC-IMS) is presented. Thirteen character impact compounds imparting different chemical properties are studied to understand capabilities and limitations of the method. It was shown for the first time that the temperature of the IMS sample inlet can be increased up to 200 °C without instrumental constraints. Linear calibration was possible for eleven of the thirteen compounds with one decade dynamic range. The limit of detection and quantitation were 2.1–63.0 ppb and 7.2–210.1 ppb, respectively. Diacetyl could be detected in negative polarity mode of IMS, however with lower precision compared to the compounds detected in positive mode. Limitations of the method were short HS-SPME extraction time, which in the case of caproic acid was not sufficient for reliable quantification. Additionally, δ-decalactone could not be detected due to maximum GC temperature of 200 °C. Application of the method to determine dynamic aroma release from a dairy matrix was successfully shown for nine compounds. Analysis of complex food matrix was performed with similar precision compared to analysis in aqueous solution, thus proving high robustness of the method towards matrix effects.show moreshow less

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Metadaten
Author:Christine F. ThomasORCiDGND, Ellen Zeh, Selina Dörfel, Yanyan Zhang, Jörg Hinrichs
DOI:https://doi.org/10.1007/s00216-021-03222-w
Identifier:1618-2650 OPAC HS OPAC extern
Identifier:1618-2642 OPAC HS OPAC extern
Parent Title (English):Analytical and Bioanalytical Chemistry
Publisher:Springer
Place of publication:Berlin, Heidelberg
Document Type:Article
Language:English
Date of Publication (online):2021/03/02
Year of first Publication:2021
Volume:413
Number of pages:10 Seiten
First Page:2577
Last Page:2586
Institutes:Fakultät Maschinenbau
KLEVERTEC
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie
Publication Lists:Borsum, Christine
Publication reviewed:begutachtet
Release Date:2024/08/23
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