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Berührungslose Interaktion gewinnt für moderne Anwendungen immer mehr an Bedeutung und ermöglicht eine intuitivere Bedienung von Computersystemen. Im Rahmen dieser Arbeit wird daher ein Erkennungs- und Tracking-Verfahren auf seine Vorteile hin untersucht. Dieses Verfahren wird schließlich in eine Robotersteuerung integriert, sodass es möglich ist, einen kleinen fahrbaren, Roboter beispielsweise durch einen Parcours zu lenken.
Many different cell types are able to migrate by formation of a thin actin-based cytoskeletal extension. Recently, it became evident that this extension consists of two distinct substructures, designated lamellipodium and lamellum, which differ significantly in their kinetic and kinematic properties as well as their biochemical composition. We developed a stochastic two-dimensional computer simulation that includes chemical reaction kinetics, G-actin diffusion, and filament transport to investigate the formation of growing actin networks in migrating cells. Model parameters were chosen based on experimental data or theoretical considerations. In this work, we demonstrate the system's ability to form two distinct networks by self-organization. We found a characteristic transition in mean filament length as well as a distinct maximum in depolymerization flux, both within the first 1-2 microm. The separation into two distinct substructures was found to be extremely robust with respect to initial conditions and variation of model parameters. We quantitatively investigated the complex interplay between ADF/cofilin and tropomyosin and propose a plausible mechanism that leads to spatial separation of, respectively, ADF/cofilin- or tropomyosin-dominated compartments. Tropomyosin was found to play an important role in stabilizing the lamellar actin network. Furthermore, the influence of filament severing and annealing on the network properties is explored, and simulation data are compared to existing experimental data.
We present a process to develop secure software with an extensive pattern-based security requirements engineering phase. It supports identifying and analyzing conflicts between different security requirements. In the design phase, we proceed by selecting security software components that achieve security requirements. The process enables software developers to systematically identify, analyze, and finally realize security requirements using security software components. We illustrate our approach by a lawyer agency software example.
Problem frames are patterns for analyzing, structuring, and characterizing software development problems. This paper presents a formal metamodel for problem frames expressed in UML class diagrams and using the formal specification notation OCL. That metamodel clarifies the nature of the different syntactical elements of problem frames, as well as the relations between them. It provides a framework for syntactical analysis and semantic validation of newly defined problem frames, and it prepares the ground for tool support for the problem frame approach.