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The use of highly expressed FTH1 as carrier protein for cytosolic targeting in Hansenula polymorpha

  • The iron storage protein ferritin is a member of the non-heme iron protein family. It can store and release iron, therefore it prevents the cell from damage caused by iron-dioxygen reactions as well as it provides iron for biological processing. To study whether the human ferritin heavy chain (FTH1) can be expressed in Hansenula polymorpha, we integrated an expression cassette for FTH1 and analyzed the protein expression. We found very efficient expression of FTH1 and obtained yields up to 1.9 g/L under non-optimized conditions. Based on this result we designed a FTH1-PTH fusion protein to successfully express the parathyroid hormone fragment 1-34 (PTH) for the first time intracellular in H. polymorpha.

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Metadaten
Author:Eva EilertORCiD, Cornelis P. Hollenberg, Michael Piontek, Manfred Suckow
Qualitätssicherung:peer reviewed
Fachbereich/Einrichtung:Hochschule Düsseldorf / Hochschulbibliothek
Document Type:Article
Year of Completion:2012
Language of Publication:English
Publisher:Elsevier
Parent Title (English):Journal of biotechnology
Volume:159
Issue:3
First Page:172
Last Page:176
Related URL:http://www.ncbi.nlm.nih.gov/pubmed/22212821
DOI:https://doi.org/10.1016/j.jbiotec.2011.12.014
Licence (German):keine Lizenz - nur Metadaten
Release Date:2020/12/16
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