TY - JOUR A1 - Bayer, Markus A1 - Pabst, Andrea T1 - Heroes and victims: economies of entitlement after violent pasts JF - Peacebuilding Y1 - 2018 U6 - https://doi.org/10.1080/21647259.2017.1303868 N1 - The research for the article was conducted as part of the project ‘Nonviolent Resistance and Democratic Consolidation’ funded by the German Research Foundation. We would like to thank Benjamin T. Shannon, Peter Finkenbusch, Felix Bethke, Daniel Lambach and the two anonymous reviewers for commenting and editing. VL - 6 IS - 1 SP - 49 EP - 64 PB - Taylor & Francis ER - TY - JOUR A1 - Dehmer, Joanna A1 - Niemann, Jörg T1 - Value Chain Management Through Cloud-based Platforms JF - Procedia - Social and Behavioral Sciences Y1 - 2018 U6 - https://doi.org/10.1016/j.sbspro.2018.03.021 N1 - PII: S1877042818300211 VL - 238 SP - 177 EP - 181 PB - Elsevier ER - TY - JOUR A1 - Bernhardt, Anne-Julchen A1 - Leeser, Jörg T1 - NEUBAU: Flexible Self-Build Cities in Germany JF - Architectural Design Y1 - 2017 U6 - https://doi.org/10.1002/ad.2213 VL - 87 IS - 5 SP - 30 EP - 37 PB - Wiley ER - TY - JOUR A1 - Bhattacharyya, Suvanjan A1 - Chattopadhyay, Himadri A1 - Guin, Anindya A1 - Benim, Ali Cemal T1 - Investigation of Inclined Turbulators for Heat Transfer Enhancement in a Solar Air Heater JF - Heat Transfer Engineering Y1 - 2019 U6 - https://doi.org/10.1080/01457632.2018.1474593 VL - 40 IS - 17/18 SP - 1451 EP - 1460 PB - Taylor & Francis ER - TY - JOUR A1 - Deckert, Carsten A1 - Schomaker, Rahel M. T1 - Cultural Impacts on National Innovativeness: Not Every Cultural Dimension Is Equal JF - Cross-Cultural Research Y1 - 2019 U6 - https://doi.org/10.1177/1069397118799700 VL - 53 IS - 2 SP - 186 EP - 214 PB - sage journals ER - TY - THES A1 - Eilert, Eva T1 - New applications of heterologous gene expression in Hansenula polymorpha for protein and metabolite production N2 - The methylotrophic yeast Hansenula polymorpha has been established as a highly efficient expression system. Strong promoters derived from the methanol metabolism pathway and mitotically stable high-copy integration have made this organism very effective for heterologous protein expression. To improve the processing of secretory proteins targeted by the MFα prepro sequence, variations in the amino acid composition near the processing site of Kex2p were investigated (Manuscript I). The insertion of Ala-Pro between MFα prepro sequence and protein of interest leads to an improved processing of the signal sequence in case of interleukin-6 (IL-6) and interferon-α 2a (IFNα-2a). Modeling of the transition sequences confirmed a better exposition of the Kex2p-cleavage site in dependency of Ala-Pro insertions. Analysis of the secreted proteins revealed activity of an Ala-Pro specific endoprotease. Partial removal of the N-terminal Ala-Pro dipeptide was also detected during IFNα-2a processing. The putative protease responsible for the processing of Ala-Pro dipeptides was identified by gene deletion. Significant similarities with Saccharomyces cerevisiae Ste13p were identified. To generally improve productivity in the H. polymorpha system new fusion protein strategies were investigated (Manuscript II). The recombinantly strongly expressed cytosolic ferritin heavy chain (FTH1) was analyzed with regard to its potential as fusion partner for difficult-to-express target proteins. A fragment of the parathyroid hormone (PTH) was chosen to explore whether fusion to FTH1 enhances PTH stability. We could show that the expression level of this fusion protein was at least of the magnitude of that of FTH1 without fusion partner. The solubility of the fusion protein was increased in comparison to FTH1 alone and no degradation of PTH occurred when fused C-terminally to FTH1. Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. H. polymorpha was engineered to express the enzymes of the H. elongata 5-hydroxyectoine biosynthesis pathway (Manuscript III). Expression of the enzymes EctB, EctA, EctC and EctD in H. polymorpha resulted in production of 5-hydroxyectoine in gram per liter scale with almost full conversion of ectoine to 5-hydroxyectoine. We showed that 5-hydroxyectoine synthesis was influenced by feeding of methanol during cultivation. Furthermore, 5-hydroxyectoine synthesis was downregulated by the addition of methionine, lysine or threonine indicating the existence of a feedback regulated aspartate kinase in H. polymorpha. N2 - Die methylotrophe Hefe Hansenula polymorpha hat sich als effizientes System zur heterologen Genexpression etabliert. Besondere Merkmale dieses Systems sind die starken Promotoren des Methanolstoffwechsels und die Fähigkeit zur mitotisch stabilen Integration einer Expressionskassette in einer hohen Kopienzahl. Am Beispiel der MF α prepro Sequenz sollte die Prozessierung sekretorisch exprimierter Proteine verbessert werden (Manuskript I). Hierfür wurden Veränderungen in der Nähe der Kex2p Erkennungssequenz durchgeführt. Durch das Einfügen von Ala-Pro zwischen MF_ prepro Sequenz und Zielprotein konnte die Prozessierung im Fall von Interleukin-6 (IL-6) und Interferon- α 2a (IFN α -2a) verbessert werden. Eine Modellierung dieses Sequenzübergangs zeigte eine verbesserte Präsentation der Kex2p-Schnittstelle. Nach Analyse des sekretierten IL-6 wurde außerdem die Aktivität einer Ala-Pro spezifischen Endoprotease festgestellt. Auch im Falle von IFN α -2a konnte eine partielle Abspaltung des N-terminalen Ala-Pro-Dipeptids festgestellt werden. Ein Protease, welche mutmaßlich an der Prozessierung dieser Ala-Pro-Dipeptide beteiligt ist, wurde per Gendeletion identifiziert. Diese Protease wies signifikante Ähnlichkeiten mit der S. cerevisiae Ste13p auf. Zur allgemeinen Verbesserung der Ausbeute bei der Produktion heterologer Proteine wurden neue Fusionsprotein-Strategien untersucht (Manuskript II). Hierfür wurde das rekombinant cytosolisch stark exprimierte Protein Ferritin (schwere Kette, FTH1) im Hinblick auf das Potenzial als Fusionspartner für schwierig zu exprimieren Zielproteine analysiert. Ein Fragment des Parathormons (PTH) wurde gewählt, um zu untersuchen, ob die Fusion beider Proteine die Stabilität von PTH erhöht. Wir konnten zeigen, dass die Expression dieses Fusionsproteins mindestens in der Größenordnung des FTH1 ohne Fusionspartner war. Die Löslichkeit des Fusionsproteins wurde gegenüber FTH1 allein erhöht und es trat kein Abbau des PTH in Fusion mit FTH1 auf. Ectoin und 5-Hydroxyectoin gehören zur Familie der kompatiblen Solute, die hauptsächlich zur Anpassung der Zelle bei osmotischem Stress durch Anpassung des Turgors beitragen. Ein H. polymorpha-Stamm wurde entwickelt, welcher die Enzyme der 5-Hydroxyectoin-Biosynthese aus H. elongata exprimiert (Manuskript III). Die Expression der Enzyme EctB, EctA, EctC und EctD in H. polymorpha resultierte in der Produktion von 5-Hydroxyectoin im Gramm pro Liter Bereich. Es fand außerdem eine nahezu vollständige Umwandlung von Ectoin in 5-Hydroxyectoin statt. Wir konnten zeigen, dass die 5-Hydroxyectoin-Synthese durch Zugabe von Methanol während der Kultivierung beeinflusst wurde. Desweiteren wurde die 5-Hydroxyectoin-Synthese durch die Zugabe von Methionin, Lysin oder Threonin herunterreguliert, was andeutet, dass in H. polymorpha eine Feedback-regulierte Aspartatkinase existiert. T2 - Neue Ansätze der heterologen Genexpression in Hansenula polymorpha zur Protein- und Metabolitenproduktion Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:061-20130527-092604-7 PB - Institut für Molekulare Mykologie, Institut für Mikrobiologie CY - Düsseldorf ER - TY - JOUR A1 - Tophoven, Silke A1 - Reims, Nancy A1 - Tisch, Anita T1 - Vocational Rehabilitation of Young Adults with Psychological Disabilities JF - Journal of Occupational Rehabilitation N2 - Objective Vocational rehabilitation measures support youth and young adults with disabilities to obtain vocational training and to enter the labor market. In Germany, a growing number of young people with psychological disabilities in vocational rehabilitation can be observed. The study at hand focuses on this group and examines their (un-)unemployment biographies before vocational rehabilitation, their access to vocational rehabilitation and identifies their individual challenges within the process of vocational rehabilitation. Methods Using a multi-methods approach, we analyze representative administrative data of the German Federal Employment Agency as well as biographical interviews conducted with young rehabilitants. We compare the population of young rehabilitants with psychological disorders to those with other disabilities in terms of vocational rehabilitation and initial labor market entry in order to get a representative picture about their school to work transitions. Since rehabilitants with psychological disabilities tend to be older than the remaining population, analyses are stratified by age groups. In addition, qualitative in-depth interviews provide an additional and deeper understanding of specific employment barriers youth with psychological disorders have to overcome. Furthermore, the individual perspective gives insight on how the crucial transition from school to work is perceived by the population under study. Results The pathway into vocational rehabilitation of youth with psychological disorders is often characterized by obstacles in their transition from school to work. During rehabilitation, it appears essential to provide psychological stabilization along with vocational training. Although their average level of education is higher than those of other rehabilitants, labor market transition after (often company-external) vocational training challenges many young people with psychological disabilities, leaving many of them with comparatively poor labor market prospects. Conclusions Young persons with psychological disabilities, who come from regular schools or dropped out from regular school or university, seem to find their way to vocational rehabilitation more indirectly. Furthermore, vocational rehabilitation itself is often prolonged for those with psychological disabilities possibly due to a corresponding stabilization process. However, vocational rehabilitation can be a core element within the stabilization process of a psychological disease. Y1 - 2019 U6 - https://doi.org/10.1007/s10926-018-9773-y VL - 29 IS - 1 SP - 150 EP - 162 PB - Springer ER - TY - JOUR A1 - Unger, Stefanie A1 - Tisch, Anita A1 - Tophoven, Silke T1 - Age and gender differences in the impact of labour-market transitions on subjective health in Germany JF - Scandinavian journal of public health Y1 - 2018 U6 - https://doi.org/10.1177/1403494817738430 VL - 46 IS - 19 SP - 49 EP - 64 PB - Sage ER - TY - RPRT A1 - Tophoven, Silke A1 - Wurdack, Anja A1 - Rauch, Angela A1 - Munkert, Casandra A1 - Bauer, Ulrike T1 - lidA - leben in der Arbeit: German cohort study on work, age and health Documentation for waves 1 and 2 N2 - The study “lidA – leben in der Arbeit. German cohort study on work, age and health” was a two-wave panel study funded by the Federal Ministry of Education and Research and examines the relationship between work, health and work participation among aging employees from an interdisciplinary perspective (www.lida-studie.de). The lidA study was carried out by an interdisciplinary consortium consisting of the Institute for Safety Technology at Bergische University Wuppertal, the Institute for Employment Research (IAB), Nuremberg, the Institute of Social Medicine and Health Economics at Otto-von-Guericke-University Magdeburg, the Institute of the History, Philosophy and Ethics of Medicine at Ulm University, infas Institute for Applied Social Sciences, Bonn and the Federal Institute for Occupational Safety and Health (BAuA), Berlin, as an associated partner. lidA contains a representative sample of persons belonging to the 1959 and 1965 birth cohorts and employed on the reference date for sampling, 31 December 2009. They were interviewed personally (CAPI) in 2011 (wave 1; N=6,585) and 2014 (wave 2; N=4,244). The data contain information about work and current employment relationship characteristics, work demands, workloads, subjective health indicators, socioeconomic status, household context and allow both cross-sectional and longitudinal analysis. This dataset is now available as a Scientific Use File for the scientific community via the Research Data Centre of the Federal Employment Agency at the Institute for Employment Research. The report contains information on data collection, data editing, anonymisation and the description of the variables of the two waves. KW - age KW - Cohort study KW - data documentation KW - employment KW - subjective health KW - Working conditions KW - workloads Y1 - 2016 UR - http://doku.iab.de/fdz/reporte/2016/DR_01-16_EN.pdf VL - FDZ Datenreport IS - 01/2016 ENG ER - TY - JOUR A1 - Rose, Uwe A1 - Schiel, Stefan A1 - Schröder, Helmut A1 - Kleudgen, Martin A1 - Tophoven, Silke A1 - Rauch, Angela A1 - Freude, Gabriele A1 - Müller, Grit T1 - The study of mental health at work. design and sampling JF - Scandinavian journal of public health Y1 - 2017 UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598876/ VL - 45 IS - 6 SP - 584 EP - 594 PB - Sage ER -