TY - JOUR A1 - Adamatzky, Andrew A1 - Schnauß, Jörg A1 - Huber, Florian T1 - Actin droplet machine JF - Royal Society Open Science N2 - The actin droplet machine is a computer model of a three-dimensional network of actin bundles developed in a droplet of a physiological solution, which implements mappings of sets of binary strings. The actin bundle network is conductive to travelling excitations, i.e. impulses. The machine is interfaced with an arbitrary selected set of k electrodes through which stimuli, binary strings of length k represented by impulses generated on the electrodes, are applied and responses are recorded. The responses are recorded in a form of impulses and then converted to binary strings. The machine's state is a binary string of length k: if there is an impulse recorded on the ith electrode, there is a '1' in the ith position of the string, and '0' otherwise. We present a design of the machine and analyse its state transition graphs. We envisage that actin droplet machines could form an elementary processor of future massive parallel computers made from biopolymers. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-34675 UR - http://www.ncbi.nlm.nih.gov/pubmed/31903204 SN - 2054-5703 N1 - A.A. was partially supported by EPSRC grant no. EP/P016677/1. The authors declare that they have no competing interests. VL - 6 IS - 12 PB - Royal Soc. Publ. ER - TY - JOUR A1 - Adamatzky, Andrew A1 - Huber, Florian A1 - Schnauß, Jörg T1 - Computing on actin bundles network JF - Scientific Reports N2 - Actin filaments are conductive to ionic currents, mechanical and voltage solitons. These travelling localisations can be utilised to generate computing circuits from actin networks. The propagation of localisations on a single actin filament is experimentally unfeasible to control. Therefore, we consider excitation waves propagating on bundles of actin filaments. In computational experiments with a two-dimensional slice of an actin bundle network we show that by using an arbitrary arrangement of electrodes, it is possible to implement two-inputs-one-output circuits. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-34665 UR - http://www.ncbi.nlm.nih.gov/pubmed/31685834 SN - 2045-2322 VL - 9 IS - 1 PB - Springer Nature ER - TY - JOUR A1 - Migueles, Jairo H. A1 - Rowlands, Alex V. A1 - Huber, Florian A1 - Sabia, Séverine A1 - van Hees, Vincent T. T1 - GGIR: A Research Community–Driven Open Source R Package for Generating Physical Activity and Sleep Outcomes From Multi-Day Raw Accelerometer Data JF - Journal for the Measurement of Physical Behaviour Y1 - 2019 U6 - https://doi.org/10.1123/jmpb.2018-0063 SN - 2575-6605 VL - 2 IS - 3 SP - 188 EP - 196 PB - Human Kinetics ER - TY - JOUR A1 - Beniddir, Mehdi A. A1 - Kang, Kyo Bin A1 - Genta-Jouve, Grégory A1 - Huber, Florian A1 - Rogers, Simon A1 - van der Hooft, Justin J. J. T1 - Advances in decomposing complex metabolite mixtures using substructure- and network-based computational metabolomics approaches JF - Natural Product Reports Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-34772 SN - 1460-4752 VL - 38 IS - 11 SP - 1967 EP - 1993 PB - The Royal Society of Chemistry ER - TY - JOUR A1 - Huber, Florian A1 - Strehle, Dan A1 - Schnauß, Jörg A1 - Käs, Josef T1 - Formation of regularly spaced networks as a general feature of actin bundle condensation by entropic forces JF - New Journal of Physics KW - DOAJ Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-34833 SN - 1367-2630 VL - 17 IS - 4 PB - IOP Publishing ER - TY - JOUR A1 - Huber, Florian A1 - Verhoeven, Stefan A1 - Meijer, Christiaan A1 - Spreeuw, Hanno A1 - Castilla, Efraín A1 - Geng, Cunliang A1 - van der Hooft, Justin J. J. A1 - Rogers, Simon A1 - Belloum, Adam A1 - Diblen, Faruk A1 - Spaaks, Jurriaan H. T1 - matchms - processing and similarity evaluation of mass spectrometry data JF - Journal of Open Source Software KW - DOAJ Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-34856 SN - 2475-9066 VL - 5 IS - 52 PB - Cold Spring Harbor Laboratory ER - TY - JOUR A1 - Huber, Florian A1 - Käs, Josef A1 - Stuhrmann, Björn T1 - Growing actin networks form lamellipodium and lamellum by self-assembly JF - Biophysical Journal N2 - Many different cell types are able to migrate by formation of a thin actin-based cytoskeletal extension. Recently, it became evident that this extension consists of two distinct substructures, designated lamellipodium and lamellum, which differ significantly in their kinetic and kinematic properties as well as their biochemical composition. We developed a stochastic two-dimensional computer simulation that includes chemical reaction kinetics, G-actin diffusion, and filament transport to investigate the formation of growing actin networks in migrating cells. Model parameters were chosen based on experimental data or theoretical considerations. In this work, we demonstrate the system's ability to form two distinct networks by self-organization. We found a characteristic transition in mean filament length as well as a distinct maximum in depolymerization flux, both within the first 1-2 microm. The separation into two distinct substructures was found to be extremely robust with respect to initial conditions and variation of model parameters. We quantitatively investigated the complex interplay between ADF/cofilin and tropomyosin and propose a plausible mechanism that leads to spatial separation of, respectively, ADF/cofilin- or tropomyosin-dominated compartments. Tropomyosin was found to play an important role in stabilizing the lamellar actin network. Furthermore, the influence of filament severing and annealing on the network properties is explored, and simulation data are compared to existing experimental data. KW - Open Archive Y1 - 2008 UR - http://www.ncbi.nlm.nih.gov/pubmed/18708450 U6 - https://doi.org/10.1529/biophysj.108.134817 SN - 1542-0086 VL - 95 IS - 12 SP - 5508 EP - 5523 PB - Biophysical Society ER - TY - JOUR A1 - Huber, Florian A1 - Käs, Josef T1 - Self-regulative organization of the cytoskeleton JF - Cytoskeleton N2 - Despite its impressive complexity the cytoskeleton succeeds to persistently organize itself and thus the cells' interior. In contrast to classical man-made machines, much of the cellular organization originates from inherent self-assembly and self-organization allowing a high degree of autonomy for various functional units. Recent experimental and theoretical studies revealed numerous examples of cytoskeleton components that arrange and organize in a self-regulative way. In the present review we want to shortly summarize some of the principle mechanisms that are able to inherently trigger and regulate the cytoskeleton organization. Although taken individually most of these regulative principles are rather simple with intuitively predictable consequences, combinations of two or more of these mechanisms can quickly give rise to very complex, unexpected behavior and might even be able to explain the formation of different functional units out of a common pool of available building blocks. Y1 - 2011 UR - http://www.ncbi.nlm.nih.gov/pubmed/21438165 U6 - https://doi.org/10.1002/cm.20509 SN - 1949-3592 N1 - Acknowledgment We would like to thank Dan Strehle for critical reading ofthis manuscript. This work was funded by the Leipzig School of Natural Sciences ‘‘BuildMoNa’’ as well as bythe Sa¨chsische Aufbaubank project number 13403 VL - 68 IS - 5 SP - 259 EP - 265 PB - Wiley ER - TY - JOUR A1 - Stuhrmann, Björn A1 - Huber, Florian A1 - Käs, Josef T1 - Robust organizational principles of protrusive biopolymer networks in migrating living cells JF - Plos One N2 - Cell migration is associated with the dynamic protrusion of a thin actin-based cytoskeletal extension at the cell front, which has been shown to consist of two different substructures, the leading lamellipodium and the subsequent lamellum. While the formation of the lamellipodium is increasingly well understood, organizational principles underlying the emergence of the lamellum are just beginning to be unraveled. We report here on a 1D mathematical model which describes the reaction-diffusion processes of a polarized actin network in steady state, and reproduces essential characteristics of the lamellipodium-lamellum system. We observe a steep gradient in filament lengths at the protruding edge, a local depolymerization maximum a few microns behind the edge, as well as a differential dominance of the network destabilizer ADF/cofilin and the stabilizer tropomyosin. We identify simple and robust organizational principles giving rise to the derived network characteristics, uncoupled from the specifics of any molecular implementation, and thus plausibly valid across cell types. An analysis of network length dependence on physico-chemical system parameters implies that to limit array treadmilling to cellular dimensions, network growth has to be truncated by mechanisms other than aging-induced depolymerization, e.g., by myosin-associated network dissociation at the transition to the cell body. Our work contributes to the analytical understanding of the cytoskeletal extension's bisection into lamellipodium and lamellum and sheds light on how cells organize their molecular machinery to achieve motility. KW - DOAJ Y1 - 2011 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-34901 SN - 1932-6203 N1 - Funding: This work has been supported by the Leipzig Graduate College ‘‘GK 1097 InterNeuro - Interdisciplinary Approaches in Cellular Neuroscience’’ (http:// www.uni-leipzig.de/˜ineuro/) and the Graduate School ‘‘Leipzig School of Natural Sciences - Building with Molecules and Nano-objects (BuildMoNa)’’ (http://www. buildmona.de/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. VL - 6 IS - 1 PB - Public Library of Science (PLoS) ER - TY - JOUR A1 - Preciado López, Magdalena A1 - Huber, Florian A1 - Grigoriev, Ilya A1 - Steinmetz, Michel O. A1 - Akhmanova, Anna A1 - Koenderink, Gijsje H. A1 - Dogterom, Marileen T1 - Actin-microtubule coordination at growing microtubule ends JF - Nature Communications N2 - To power dynamic processes in cells, the actin and microtubule cytoskeletons organize into complex structures. Although it is known that cytoskeletal coordination is vital for cell function, the mechanisms by which cross-linking proteins coordinate actin and microtubule activities remain poorly understood. In particular, it is unknown how the distinct mechanical properties of different actin architectures modulate the outcome of actin-microtubule interactions. To address this question, we engineered the protein TipAct, which links growing microtubule ends via end-binding proteins to actin filaments. We show that growing microtubules can be captured and guided by stiff actin bundles, leading to global actin-microtubule alignment. Conversely, growing microtubule ends can transport, stretch and bundle individual actin filaments, thereby globally defining actin filament organization. Our results provide a physical basis to understand actin-microtubule cross-talk, and reveal that a simple cross-linker can enable a mechanical feedback between actin and microtubule organization that is relevant to diverse biological contexts. KW - DOAJ Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-34878 SN - 2041-1723 VL - 5 PB - Springer Nature ER - TY - JOUR A1 - Johanning, M. A1 - Braun, Alexander A1 - Timoney, N. A1 - Elman, V. A1 - Neuhauser, W. A1 - Wunderlich, Chr T1 - Individual addressing of trapped ions and coupling of motional and spin states using RF radiation JF - Physical review letters N2 - Individual electrodynamically trapped and laser cooled ions are addressed in frequency space using radio-frequency radiation in the presence of a static magnetic field gradient. In addition, an interaction between motional and spin states induced by an rf field is demonstrated employing rf optical double resonance spectroscopy. These are two essential experimental steps towards realizing a novel concept for implementing quantum simulations and quantum computing with trapped ions. Y1 - 2009 UR - http://www.ncbi.nlm.nih.gov/pubmed/19257664 U6 - https://doi.org/10.1103/PhysRevLett.102.073004 SN - 1079-7114 VL - 102 IS - 7 PB - American Physical Society ER - TY - JOUR A1 - Johanning, M. A1 - Braun, Alexander A1 - Eiteneuer, D. A1 - Paape, C. A1 - Balzer, C. A1 - Neuhauser, W. A1 - Wunderlich, C. T1 - Resonance-enhanced isotope-selective photoionization of YbI for ion trap loading JF - Applied Physics B Y1 - 2011 UR - https://arxiv.org/pdf/0712.0969.pdf U6 - https://doi.org/10.1007/s00340-011-4502-7 SN - 1432-0649 VL - 103 IS - 2 SP - 327 EP - 338 PB - Springer Nature ER - TY - JOUR A1 - Voss, Karsten A1 - Musall, Eike A1 - Lichtmeß, Markus T1 - From Low Energy to Net Zero-Energy Buildings: Status and Perspectives JF - Journal of Green Building N2 - “Net Zero-Energy Building” has become a popular catchphrase to describe the synergy between energy-efficient building and renewable energy utilisation to achieve a balanced energy budget over an annual cycle. Taking into account the energy exchange with a grid overcomes the limitations of energy-autonomous buildings with the need for seasonal energy storage on-site. Although the expression, “Net Zero-Energy Building,” appears in many energy policy documents, a harmonised definition or a standardised balancing method is still lacking. This paper reports on the background and the various effects influencing the energy balance approach. After discussing the national energy code framework in Germany, a harmonised terminology and balancing procedure is proposed. The procedure takes not only the energy balance but also energy efficiency and load matching into account. KW - Eigene Methodik Y1 - 2011 U6 - https://doi.org/10.3992/jgb.6.1.46 VL - 6 IS - 1 SP - 46 EP - 57 PB - College Publishing ER - TY - JOUR A1 - Voss, Karsten A1 - Musall, Eike T1 - Null- und Plusenergiegebäude: Allgemeine Bilanzierungsverfahren und Schnittstellen zur normativen Praxis in Deutschland JF - EnEVaktuell N2 - Die Begriffe „Nullenergiehaus“ oder „Plusenergiehaus“ sind in den letzten Jahren national und international zum Inbegriff für die konsequente Zusammenführung von Maßnahmen zur drastischen Energieeinsparung und optimierter, dezentraler Nutzung erneuerbarer Energien geworden. Dabei geht es nicht um energieautarke Gebäude und die dabei große Herausforderung der saisonalen Energiespeicherung. Grundidee ist, dass die von einem Gebäude in ein Netz eingespeiste Energiemenge in der Jahresbilanz mindestens dem Energiebezug entspricht. Sowohl im Kontext der Fortführung der europäischen Gebäuderichtline als auch im aktuellen Energiekonzept und Energieforschungsprogramm der Bundesregierung wird das Thema besetzt. KW - Nullenergiehaus KW - Energieinsparung Y1 - 2011 UR - https://www.btga-arch.uni-wuppertal.de/fileadmin/architektur/btga-arch/btga_veroeffentlichungen/btga_zeitschrift/Null-_und_Plusenergiegeb%EF%BF%BDude_EnEVaktuell_12_2011.pdf IS - IV SP - 3 EP - 5 PB - DIN Deutsches Institut für Normung e.V. ER - TY - JOUR A1 - Musall, Eike A1 - Voss, Karsten T1 - Renewable Energy Ratio in Net Zero Energy Buildings JF - REHVA Journal KW - Bilanzierung KW - Definitionsbeschrieb Y1 - 2014 UR - https://www.rehva.eu/rehva-journal/chapter/renewable-energy-ratio-in-net-zero-energy-buildings VL - 51 IS - 3 SP - 14 EP - 18 PB - REHVA ER - TY - JOUR A1 - de Bánffy-Hall, Alicia A1 - Hill, Burkhard T1 - Community Music: Eine Einführung JF - KULTURELLE BILDUNG ONLINE Y1 - 2017 UR - https://doi.org/10.25529/92552.79 U6 - https://doi.org/10.25529/92552.79 PB - Bundesakademie für Kulturelle Bildung ER - TY - JOUR A1 - de Bánffy-Hall, Alicia A1 - Hill, Burkhard T1 - Community Music: Eine Einführung JF - kubi-online Y1 - 2017 UR - https://www.kubi-online.de/artikel/community-music-einfuehrung ER - TY - JOUR A1 - Hörner, Fernand T1 - La flexibilité historique de l’aura : de Walter Benjamin à Radiohead JF - Communication & Langages Y1 - 2015 U6 - https://doi.org/10.4074/S0336150015012041 N1 - © NecPlus. Tous droits réservés pour tous pays VL - 2 IS - 184 SP - 61 EP - 77 PB - cairn-int.info ER - TY - JOUR A1 - Hiesinger, Karolin A1 - Tophoven, Silke T1 - Job requirement level, work demands, and health: a prospective study among older workers JF - International Archives of Occupational and Environmental Health KW - Mediation KW - Mental health KW - Older workers KW - Physical health KW - Physical work demands KW - Psychosocial work demands Y1 - 2019 UR - https://rdcu.be/b5E30 U6 - https://doi.org/10.1007/s00420-019-01451-2 VL - 92 SP - 1139 EP - 1149 PB - Springer Nature ER - TY - JOUR A1 - Hiesinger, Karolin A1 - Tophoven, Silke A1 - March, Stefanie T1 - Geschlechtsspezifische Verzerrungen bei der Erfassung von Depressivität JF - Prävention und Gesundheitsförderung KW - BDI-V KW - Befragungsmodus KW - Bevölkerungsumfragen KW - Depressivität KW - Geschlechtsspezifische Verzerrung Y1 - 2018 UR - https://rdcu.be/b5FXp U6 - https://doi.org/10.1007/s11553-017-0634-x VL - 13 IS - 3 SP - 211 EP - 217 PB - Springer Nature ER -