TY - CHAP A1 - Deckert, Carsten A1 - Söhnitz, Stefanie ED - Kangaslampi, R. ED - Langie, G. ED - Järvinen, H.-M. ED - Nagy, B. T1 - Words Don't Come Easy to Me – Teaching Academic Writing to Engineering Students in the Age Of AI T2 - SEFI 53rd Annual Conference (SEFI 2025), Tampere, Finland, 15-18 September 2025 N2 - The competence of academic writing is currently still a central requirement for obtaining an academic degree. However, in teaching academic writing, lecturers have to deal with the two challenges of decreasing reading competence in young people and increasing use of artificial intelligence (AI) in reading and writing assignments. In engineering in particular, literacy understood as reading and writing competence is essential - whether for understanding complex tasks, analysing case studies or dealing with technical documents such as the product requirements document. Against this background, it is crucial to develop strategies and discuss modern approaches to specifically promote students' reading and writing skills and thus create an essential basis for successful scientific work and problem-solving. This workshop aims to stimulate an interactive exchange and develop practical solutions in the three core areas strengthening reading competence, developing academic writing skills, and substituting for reading and writing assessments. KW - Literacy KW - Reading Competence KW - Writing Competence KW - Academic Writing KW - Critical Thinking Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-58627 SN - 978-2-87352-029-8 PB - SEFI CY - Tampere ER - TY - CHAP A1 - Boelter, Sarah A1 - Claas, Oliver A1 - Dähne, Judith A1 - Heuer, Jan-Ocko A1 - Kahlisch, Mareen A1 - Koch, Katharina A1 - Krüger, Frank A1 - Lazarescu, Roxana A1 - Lehmann, Sebastian B.C. A1 - Lubahn, Antje A1 - Pilch, Shanna A1 - Reichwald, Julian A1 - Reiter, Martin A1 - Schirilò, Darina A1 - Schimmer, Thomas M. A1 - Schmidt, Diana A1 - Schmitt, Kathrin A1 - Schreiber, Felix A1 - Schulz, Dörthe A1 - Stiller, Gabriele A1 - Werth, Robert A1 - Wolff, Ian T1 - The Role of Universities of Applied Sciences in the Development of a National Research Data Infrastructure: Situation and Potentials of Universities of Applied Sciences from the Perspective of the Network FDM@HAW [Manuskript und Präsentation] T2 - 2nd Conference on Research Data Infrastructure (CoRDI) , Aachen, Germany, 26-28 August 2025 (Session 6. Context) KW - Nationale Forschungsdateninfrastruktur (NFDI) e.V. KW - Angewandte Forschung KW - Forschungsdatenmanagement KW - Fachhochschule Y1 - 2025 UR - https://web.archive.org/web/20250829210115/https://www.nfdi.de/wp-content/uploads/2025/08/CoRDI-2025-Conference-Programme_final.pdf UR - https://nbn-resolving.org/urn:nbn:de:hbz:due62-opus-56313 U6 - https://doi.org/10.5281/zenodo.17055442 PB - Zenodo ER - TY - CHAP A1 - Boelter, Sarah A1 - Claas, Oliver A1 - Dähne, Judith A1 - Heuer, Jan-Ocko A1 - Kahlisch, Mareen A1 - Koch, Katharina A1 - Krüger, Frank A1 - Lazarescu, Roxana A1 - Lubahn, Antje A1 - Reichwald, Julian A1 - Reiter, Martin A1 - Schilirò, Darina A1 - Schimmer, Thomas M. A1 - Schmidt, Diana A1 - Schmitt, Kathrin A1 - Schreiber, Felix A1 - Schulz, Dörthe A1 - Stiller, Gabriele A1 - Werth, Robert A1 - Ian, Wolff ED - Sure-Vetter, York ED - Groth, Paul T1 - The Role of Universities of Applied Sciences in the Development of a national Research Data Infrastructure T2 - 2nd Conference on Research Data Infrastructure (CoRDI) N2 - The presentation highlights the role of Universities of Applied Sciences (UAS) in research and their potential for the development of a National Research Data Infrastructure (NFDI). To address the growing need for structured research data management (RDM) at UASs, the FDM@HAW network has emerged as a potential connector between NFDI and UAS landscape. It aims to raise awareness of NFDI services across a diverse UAS network, and articulate UAS-specific requirements back to NFDI. In doing so, it strengthens mutual integration and fosters targeted support. With over 200 institutions [1], UASs constitute a significant part of the German science system. Traditionally focused on teaching, UASs have become drivers of applied research and regional innovation. Their contributions to education and research make them essential actors in shaping RDM in Germany. Research at UASs benefits business, science, and society. In many federal states, the right to award doctorates further reinforces their research activities. Strengthening RDM competencies is important to ensure that valuable research data from UAS meet the FAIR principles. Furthermore, employers benefit from graduates equipped with strong data literacy. Despite their potential, UASs remain underrepresented in the NFDI (and partly in the RDM community). Low participation in NFDI consortia and limited association membership indicate a broader lack of engagement, largely due to low awareness of NFDI among UAS researchers and administrations [2, p.42] [3, p.6]. The NFDI structure report 2024 addresses this gap and calls for broader contact to UASs [4, p.27]. Structural challenges limit UAS engagement in RDM and the NFDI, including high teaching loads, minor resources, and partnerships prioritizing data protection over openness or commercial interests. In response, many UASs have implemented strategic networking in research, teaching, and infrastructure to overcome resource limitations. This approach also drives the development of RDM structures, often via projects or state-level initiatives. The Federal Ministry of Education and Research supports 14 projects [5] through its funding guideline for RDM at UASs. This initiative led to the establishment of the FDM@HAW network in 2023, connecting these projects. Since its formation, the network has engaged in continuous exchange on RDM development and contributed to events such as Love Data Week, the FORTRAMA conference [6], Volkswagen Foundation's Digital Skills in Science Week [7] and E-Science-Tage [8]. These activities have helped sharpen the network's focus, identify shared priorities and integrate new members. Members contribute ideas, tools and services developed in RDM projects or collaborations - such as the "NFDI Survival Kit" [9] and the "NFDI-N(HAW)igator" [10] - to promote RDM awareness and inform about NFDI on a national scale. In line with NFDI's mission to "improv[e] the possibilities for using data for science and society" [11], FDM@HAW seeks to disseminate knowledge of and strengthen competence in delving into these possibilities. The network provides valuable support to activate a target group that has largely been untapped. Close cooperation between NFDI and FDM@HAW will then contribute to the successful development of a nationwide RDM ecosystem. In the presentation, proposals for cooperation will be outlined. (The references [1]-[11] are given in the PDF abstract according to the template). KW - Nationale Forschungsdateninfrastruktur (NFDI) e.V. KW - Angewandte Forschung KW - Forschungsdatenmanagement KW - Fachhochschule Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:due62-opus-56313 UR - https://web.archive.org/web/20250829210115/https://www.nfdi.de/wp-content/uploads/2025/08/CoRDI-2025-Conference-Programme_final.pdf PB - Zenodo ER - TY - GEN A1 - Eilert, Eva T1 - Research Data Lifecycle N2 - A data lifecycle serves as a framework for structured data management. It covers the entire data flow from planning and generation to storage and provision. Measures for structured data management can be taken at all stages of the data lifecycle in order to use data efficiently, ensure its quality and guarantee its long-term availability. This diagram visualizes the research data lifecycle, which is divided into three main areas: planning and repurposing, generation and processing, and storage and provision. Each area comprises specific tasks and processes for handling data. The first phase, “Planning and Repurposing”, contains two key elements. The first is the planning of the research project, in which the objectives, methods and resources are defined. Secondly, it involves researching and reusing existing data in order to assess its usefulness and potential for the project at hand. The second phase, “Generation and processing”, consists of three elements, which are mapped in a separate circular process. First, data is collected and recorded, either through experimental studies, surveys or other methods. The transition from the first phase to the second phase takes place via this element, as this is where the basic generation of own data within the research begins. Secondly, the collected data is stored and organized to ensure its integrity and availability. Thirdly, the relevant information is processed, analyzed and selected. The transition from the second phase to the third phase takes place from this element, as the knowledge gained is prepared for further storage and provision. Overall, the second phase forms a continuous cycle. The third phase “Storage and Provision” consists of three elements. Firstly, the research results are published, be it in scientific journals, repositories or other relevant publication venues. Secondly, the data is archived to ensure its long-term availability and integrity. Thirdly, access to the research results is created, for example through selective access for certain user groups or increased visibility. KW - Forschungsdatenmanagement Y1 - 2025 U6 - https://doi.org/10.5281/zenodo.14752723 N1 - German version available under: https://doi.org/10.5281/zenodo.8087764 This graphic was created as part of the project module of the certificate course Research Data Management 2022/2023 (a cooperative course of the Landesinitiative für Forschungsdatenmanagement NRW - fdm.nrw, ZB MED - Informationszentrum Lebenswissenschaften and ZBIW). The graphic is available in 3 formats (PNG, SVG, AI) - colors, contours and lettering can be customized depending on the file format. PB - Zenodo ET - v1 ER - TY - JOUR A1 - Eilert, Eva A1 - Kranz, Andreas A1 - Hollenberg, Cornelis P. A1 - Piontek, Michael A1 - Suckow, Manfred T1 - Synthesis and release of the bacterial compatible solute 5-hydroxyectoine in Hansenula polymorpha JF - Journal of biotechnology N2 - Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to mainly contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition the cell's essential functions are maintained under stress conditions like high salinity, heat or aridity stress. Hansenula polymorpha was engineered to catalyze the transformation of monomeric substrates to 5-hydroxyectoine. For this purpose four genes encoding the enzymes of the 5-hydroxyectoine biosynthesis pathway of Halomonas elongata, EctA, EctB, EctC, and EctD, were inserted into the genome of H. polymorpha. Subsequently the syntheses of ectoine and 5-hydroxyectoine were analyzed and optimized. We showed that H. polymorpha is a suitable system for recombinant 5-hydroxyectoine synthesis in gram per liter scale (2.8 g L⁻¹ culture supernatant, 365 μmol/g dcw) in which almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity were achieved. Y1 - 2013 UR - http://www.ncbi.nlm.nih.gov/pubmed/23467000 U6 - https://doi.org/10.1016/j.jbiotec.2013.02.005 VL - 167 IS - 2 SP - 85 EP - 93 PB - Elsevier ER - TY - JOUR A1 - Eilert, Eva A1 - Hollenberg, Cornelis P. A1 - Piontek, Michael A1 - Suckow, Manfred T1 - The use of highly expressed FTH1 as carrier protein for cytosolic targeting in Hansenula polymorpha JF - Journal of biotechnology N2 - The iron storage protein ferritin is a member of the non-heme iron protein family. It can store and release iron, therefore it prevents the cell from damage caused by iron-dioxygen reactions as well as it provides iron for biological processing. To study whether the human ferritin heavy chain (FTH1) can be expressed in Hansenula polymorpha, we integrated an expression cassette for FTH1 and analyzed the protein expression. We found very efficient expression of FTH1 and obtained yields up to 1.9 g/L under non-optimized conditions. Based on this result we designed a FTH1-PTH fusion protein to successfully express the parathyroid hormone fragment 1-34 (PTH) for the first time intracellular in H. polymorpha. Y1 - 2012 UR - http://www.ncbi.nlm.nih.gov/pubmed/22212821 U6 - https://doi.org/10.1016/j.jbiotec.2011.12.014 VL - 159 IS - 3 SP - 172 EP - 176 PB - Elsevier ER - TY - THES A1 - Eilert, Eva T1 - New applications of heterologous gene expression in Hansenula polymorpha for protein and metabolite production N2 - The methylotrophic yeast Hansenula polymorpha has been established as a highly efficient expression system. Strong promoters derived from the methanol metabolism pathway and mitotically stable high-copy integration have made this organism very effective for heterologous protein expression. To improve the processing of secretory proteins targeted by the MFα prepro sequence, variations in the amino acid composition near the processing site of Kex2p were investigated (Manuscript I). The insertion of Ala-Pro between MFα prepro sequence and protein of interest leads to an improved processing of the signal sequence in case of interleukin-6 (IL-6) and interferon-α 2a (IFNα-2a). Modeling of the transition sequences confirmed a better exposition of the Kex2p-cleavage site in dependency of Ala-Pro insertions. Analysis of the secreted proteins revealed activity of an Ala-Pro specific endoprotease. Partial removal of the N-terminal Ala-Pro dipeptide was also detected during IFNα-2a processing. The putative protease responsible for the processing of Ala-Pro dipeptides was identified by gene deletion. Significant similarities with Saccharomyces cerevisiae Ste13p were identified. To generally improve productivity in the H. polymorpha system new fusion protein strategies were investigated (Manuscript II). The recombinantly strongly expressed cytosolic ferritin heavy chain (FTH1) was analyzed with regard to its potential as fusion partner for difficult-to-express target proteins. A fragment of the parathyroid hormone (PTH) was chosen to explore whether fusion to FTH1 enhances PTH stability. We could show that the expression level of this fusion protein was at least of the magnitude of that of FTH1 without fusion partner. The solubility of the fusion protein was increased in comparison to FTH1 alone and no degradation of PTH occurred when fused C-terminally to FTH1. Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. H. polymorpha was engineered to express the enzymes of the H. elongata 5-hydroxyectoine biosynthesis pathway (Manuscript III). Expression of the enzymes EctB, EctA, EctC and EctD in H. polymorpha resulted in production of 5-hydroxyectoine in gram per liter scale with almost full conversion of ectoine to 5-hydroxyectoine. We showed that 5-hydroxyectoine synthesis was influenced by feeding of methanol during cultivation. Furthermore, 5-hydroxyectoine synthesis was downregulated by the addition of methionine, lysine or threonine indicating the existence of a feedback regulated aspartate kinase in H. polymorpha. N2 - Die methylotrophe Hefe Hansenula polymorpha hat sich als effizientes System zur heterologen Genexpression etabliert. Besondere Merkmale dieses Systems sind die starken Promotoren des Methanolstoffwechsels und die Fähigkeit zur mitotisch stabilen Integration einer Expressionskassette in einer hohen Kopienzahl. Am Beispiel der MF α prepro Sequenz sollte die Prozessierung sekretorisch exprimierter Proteine verbessert werden (Manuskript I). Hierfür wurden Veränderungen in der Nähe der Kex2p Erkennungssequenz durchgeführt. Durch das Einfügen von Ala-Pro zwischen MF_ prepro Sequenz und Zielprotein konnte die Prozessierung im Fall von Interleukin-6 (IL-6) und Interferon- α 2a (IFN α -2a) verbessert werden. Eine Modellierung dieses Sequenzübergangs zeigte eine verbesserte Präsentation der Kex2p-Schnittstelle. Nach Analyse des sekretierten IL-6 wurde außerdem die Aktivität einer Ala-Pro spezifischen Endoprotease festgestellt. Auch im Falle von IFN α -2a konnte eine partielle Abspaltung des N-terminalen Ala-Pro-Dipeptids festgestellt werden. Ein Protease, welche mutmaßlich an der Prozessierung dieser Ala-Pro-Dipeptide beteiligt ist, wurde per Gendeletion identifiziert. Diese Protease wies signifikante Ähnlichkeiten mit der S. cerevisiae Ste13p auf. Zur allgemeinen Verbesserung der Ausbeute bei der Produktion heterologer Proteine wurden neue Fusionsprotein-Strategien untersucht (Manuskript II). Hierfür wurde das rekombinant cytosolisch stark exprimierte Protein Ferritin (schwere Kette, FTH1) im Hinblick auf das Potenzial als Fusionspartner für schwierig zu exprimieren Zielproteine analysiert. Ein Fragment des Parathormons (PTH) wurde gewählt, um zu untersuchen, ob die Fusion beider Proteine die Stabilität von PTH erhöht. Wir konnten zeigen, dass die Expression dieses Fusionsproteins mindestens in der Größenordnung des FTH1 ohne Fusionspartner war. Die Löslichkeit des Fusionsproteins wurde gegenüber FTH1 allein erhöht und es trat kein Abbau des PTH in Fusion mit FTH1 auf. Ectoin und 5-Hydroxyectoin gehören zur Familie der kompatiblen Solute, die hauptsächlich zur Anpassung der Zelle bei osmotischem Stress durch Anpassung des Turgors beitragen. Ein H. polymorpha-Stamm wurde entwickelt, welcher die Enzyme der 5-Hydroxyectoin-Biosynthese aus H. elongata exprimiert (Manuskript III). Die Expression der Enzyme EctB, EctA, EctC und EctD in H. polymorpha resultierte in der Produktion von 5-Hydroxyectoin im Gramm pro Liter Bereich. Es fand außerdem eine nahezu vollständige Umwandlung von Ectoin in 5-Hydroxyectoin statt. Wir konnten zeigen, dass die 5-Hydroxyectoin-Synthese durch Zugabe von Methanol während der Kultivierung beeinflusst wurde. Desweiteren wurde die 5-Hydroxyectoin-Synthese durch die Zugabe von Methionin, Lysin oder Threonin herunterreguliert, was andeutet, dass in H. polymorpha eine Feedback-regulierte Aspartatkinase existiert. T2 - Neue Ansätze der heterologen Genexpression in Hansenula polymorpha zur Protein- und Metabolitenproduktion Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:061-20130527-092604-7 PB - Institut für Molekulare Mykologie, Institut für Mikrobiologie CY - Düsseldorf ER - TY - JOUR A1 - Eilert, Eva A1 - Rolf, Theresa A1 - Heumaier, Andreas A1 - Hollenberg, Cornelis P. A1 - Piontek, Michael A1 - Suckow, Manfred T1 - Improved processing of secretory proteins in Hansenula polymorpha by sequence variation near the processing site of the alpha mating factor prepro sequence JF - Journal of biotechnology N2 - The literature as well as databases are ambiguous about the exact start of human interleukin-6 (IL-6)--three possibilities for the initiation of the mature protein are described. These three variants of IL-6, different in the exact initiation of the mature protein (A28, P29, or V30), were expressed in Hansenula polymorpha using the Saccharomyces cerevisiae MFα prepro sequence instead of the homologous pre sequence. All three IL-6 variants were secreted but the processing by the Kex2 protease showed significant differences. V30-IL-6 showed correctly processed material but also a molecule species of higher molecular weight indicating incomplete processing of the MFα pro peptide. P29-IL-6 did not yield any correctly processed IL-6, instead only the unprocessed pro form was found in the culture supernatant. Only A28-IL-6 led to 100% correctly processed material. N-terminal sequencing of this material revealed a start at V30--obviously the first two amino acids (Ala28-Pro29) have been removed by a so far unknown protease. Thus expression of both A28-IL-6 and V30-IL-6 as MFα prepro fusion proteins resulted in the very same mature V30-IL-6, however, the ratio of correctly processed molecules was significantly higher in the case of A28-IL-6. The expression of an MFα prepro-interferon α-2a (IFNα-2a) fusion protein in H. polymorpha leads to about 50% correctly processed molecules and 50% misprocessed forms which contain part of the pro peptide at the N-termini. The insertion of A28 and P29 of IL-6 between the pro peptide and the start of the mature IFNα-2a led to correct processing and elimination of all high molecular weight isoforms observed in earlier experiments. Y1 - 2013 UR - http://www.ncbi.nlm.nih.gov/pubmed/22982399 U6 - https://doi.org/10.1016/j.jbiotec.2012.08.024 VL - 167 IS - 2 SP - 94 EP - 100 PB - Elsevier ER - TY - PAT A1 - Eilert, Eva A1 - Suckow, Manfred A1 - Piontek, Michael T1 - A method for producing ectoine or a derivative thereof and a yeast cell for use as a host cell in such a method N2 - Klassifikationen IPC C12N15/81; C12N15/82; C12P17/12; C12P7/42; CPC C12N15/815 (EP); C12P17/12 (EP); Y1 - 2014 UR - https://depatisnet.dpma.de/DepatisNet/depatisnet?action=pdf&docid=EP000002743350A1&xxxfull=1 ER - TY - PAT A1 - Kraemer, Marco A1 - Svetlichny, Vitaly A1 - Eilert, Eva T1 - Method for producing a fermentation product: Angemeldet durch DIREVO Industrial Biotechnology GmbH N2 - Prioritätsdaten: EP15191039A·2015-10-22; US201562245054P·2015-10-22 Bibliographische IPC: C12P7/06; C12P7/14; Y1 - 2017 UR - https://depatisnet.dpma.de/DepatisNet/depatisnet?action=pdf&docid=WO002017067698A1&xxxfull=1 ER -