TY - CHAP A1 - Aelenei, Laura A1 - Musall, Eike ED - Gardes, François ED - Ayoub, Josef ED - Aelenei, Daniel ED - Aelenei, Laura ED - Scognamiglio, Alessandra T1 - Net zero energy building design fundamentals T2 - Solution sets for net zero energy buildings KW - Energiebewusstes Bauen KW - Nullenergiehaus KW - Passivhaus KW - Nachhaltigkeit KW - Energieeffizienz KW - Niedrigenergiehaus Y1 - 2017 SN - 9783433604663 U6 - https://doi.org/10.1002/9783433604663.ch2 SP - 7 EP - 38 PB - Ernst & Sohn CY - Berlin ER - TY - CHAP A1 - Marszal, Anna Joanna A1 - Bourelle, Julien S. A1 - Musall, Eike A1 - Heiselberg, Per A1 - Gustavsen, Arild A1 - Voss, Karsten T1 - Net Zero Energy Buildings - Calculation Methodologies versus National Building Codes T2 - EuroSun Conference 2010, Graz, 28.9-30.9.2010 N2 - The international cooperation project IEA SHC Task 40 / ECBCS Annex 52 “Towards Net Zero Energy Solar Buildings”, attempts to develop a common understanding and to set up the basis for an international definition framework of Net Zero Energy Buildings (Net ZEBs). Th e understanding of such buildings and how the Net ZEB status should be calculated differs in most countries. This paper presents an overview of Net ZEBs energy calculation methodologies proposed by organisations representing eight different countries: Austria, Canada, Denmark, Germany, Italy, Norway, Switzerland and the USA. The different parameters used in the calculations are discussed and the various renewable supply options considered in the methodologies are summarised graphically. Thus, the paper helps to understand different existing approaches to calculate energy balance in Net ZEBs, highlights the importance of variables selection and identify possible renewable energy supply options which may be considered in calculations. Finally, the gap between the methodology proposed by each organisation and their respective national building code is assessed; providing an overview of the possible changes building codes will need to undergo in the coming years. KW - Label Y1 - 2010 UR - https://www.researchgate.net/publication/266247323_Net_Zero_Energy_Buildings_-_Calculation_Methodologies_Versus_National_Building_Codes CY - Graz ER - TY - CHAP A1 - Musall, Eike T1 - Net Zero Energy Buildings - Definition and building concepts T2 - Net Zero-Energy Buildings Conference, Lissabon Y1 - 2012 CY - Lissabon ER - TY - GEN ED - Voss, Karsten ED - Musall, Eike T1 - Net Zero Energy Buildings: International Projects of Carbon Neutrality in Buildings KW - Definitionsbeschrieb KW - Eigene Methodik KW - KWK KW - Messdatenauswertung KW - Projektbeschreibung KW - Querschnittsauswertung KW - Sanierung KW - Solarenergie KW - Verbrauchswerte Y1 - 2013 SN - 3920034805 VL - Detail green books PB - De Gruyter CY - Berlin ER - TY - CHAP A1 - Musall, Eike A1 - Weiß, Tobias A1 - Voss, Karsten A1 - Lenoir, Aurélie A1 - Donn, Michael A1 - Cory, Shaan A1 - Garde, François T1 - Net Zero Energy Solar Buildings: An Overview and Analysis on worldwide Building Projects. EuroSun Conference 2010 N2 - This paper summarises the state of two research phases within the scope of the IEA Task 40 / Annex 52 "Towards Net Zero Energy Solar Buildings" [1]. The first objective is a cross section analysis of a comprehensive collection of more than 280 international zero energy buildings. The aim is to show trends, motives of actors, as well as their method to reach the zero energy balance. Secondly, an in-depth study shows a rough analysis and characteristics of 50 exemplary Nets ZEBs from different countries and climate regions, as well as their combinations of measures concerning energy efficiency and renewable energy supply. It is shown that diverse actors with miscellaneous motives have lead to a lot of different building variations. Typical strategies can be assigned to the typology groups "small residential building", "apartment building" and "non residential building". Net ZEBs are much more energy efficient than average buildings which were built according to national construction and energy regulations. None of the leading Net ZEB examples exist without generation of PV electricity. Trends give an outlook of current and possible future combinations of technologies and passive measures for the realization of (future) net zero energy buildings. KW - Querschnittsauswertung Y1 - 2010 UR - http://proceedings.ises.org/paper/eurosun2010/eurosun2010-0080-Musall.pdf CY - Graz ER - TY - JOUR A1 - Bernhardt, Anne-Julchen A1 - Leeser, Jörg T1 - NEUBAU: Flexible Self-Build Cities in Germany JF - Architectural Design Y1 - 2017 U6 - https://doi.org/10.1002/ad.2213 VL - 87 IS - 5 SP - 30 EP - 37 PB - Wiley ER - TY - JOUR A1 - Zellner, R. A1 - Herrmann, H. A1 - Wiesen, P. A1 - Nehr, Sascha T1 - New and Emerging Technologies of Societal Relevance: Impact on Air Quality and Climate JF - Chemie Ingenieur Technik KW - Luftreinhaltung Y1 - 2016 U6 - https://doi.org/10.1002/cite.201650083 SN - 0009-286X VL - 88 IS - 9 SP - 1257 EP - 1258 PB - Wiley ER - TY - THES A1 - Eilert, Eva T1 - New applications of heterologous gene expression in Hansenula polymorpha for protein and metabolite production N2 - The methylotrophic yeast Hansenula polymorpha has been established as a highly efficient expression system. Strong promoters derived from the methanol metabolism pathway and mitotically stable high-copy integration have made this organism very effective for heterologous protein expression. To improve the processing of secretory proteins targeted by the MFα prepro sequence, variations in the amino acid composition near the processing site of Kex2p were investigated (Manuscript I). The insertion of Ala-Pro between MFα prepro sequence and protein of interest leads to an improved processing of the signal sequence in case of interleukin-6 (IL-6) and interferon-α 2a (IFNα-2a). Modeling of the transition sequences confirmed a better exposition of the Kex2p-cleavage site in dependency of Ala-Pro insertions. Analysis of the secreted proteins revealed activity of an Ala-Pro specific endoprotease. Partial removal of the N-terminal Ala-Pro dipeptide was also detected during IFNα-2a processing. The putative protease responsible for the processing of Ala-Pro dipeptides was identified by gene deletion. Significant similarities with Saccharomyces cerevisiae Ste13p were identified. To generally improve productivity in the H. polymorpha system new fusion protein strategies were investigated (Manuscript II). The recombinantly strongly expressed cytosolic ferritin heavy chain (FTH1) was analyzed with regard to its potential as fusion partner for difficult-to-express target proteins. A fragment of the parathyroid hormone (PTH) was chosen to explore whether fusion to FTH1 enhances PTH stability. We could show that the expression level of this fusion protein was at least of the magnitude of that of FTH1 without fusion partner. The solubility of the fusion protein was increased in comparison to FTH1 alone and no degradation of PTH occurred when fused C-terminally to FTH1. Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. H. polymorpha was engineered to express the enzymes of the H. elongata 5-hydroxyectoine biosynthesis pathway (Manuscript III). Expression of the enzymes EctB, EctA, EctC and EctD in H. polymorpha resulted in production of 5-hydroxyectoine in gram per liter scale with almost full conversion of ectoine to 5-hydroxyectoine. We showed that 5-hydroxyectoine synthesis was influenced by feeding of methanol during cultivation. Furthermore, 5-hydroxyectoine synthesis was downregulated by the addition of methionine, lysine or threonine indicating the existence of a feedback regulated aspartate kinase in H. polymorpha. N2 - Die methylotrophe Hefe Hansenula polymorpha hat sich als effizientes System zur heterologen Genexpression etabliert. Besondere Merkmale dieses Systems sind die starken Promotoren des Methanolstoffwechsels und die Fähigkeit zur mitotisch stabilen Integration einer Expressionskassette in einer hohen Kopienzahl. Am Beispiel der MF α prepro Sequenz sollte die Prozessierung sekretorisch exprimierter Proteine verbessert werden (Manuskript I). Hierfür wurden Veränderungen in der Nähe der Kex2p Erkennungssequenz durchgeführt. Durch das Einfügen von Ala-Pro zwischen MF_ prepro Sequenz und Zielprotein konnte die Prozessierung im Fall von Interleukin-6 (IL-6) und Interferon- α 2a (IFN α -2a) verbessert werden. Eine Modellierung dieses Sequenzübergangs zeigte eine verbesserte Präsentation der Kex2p-Schnittstelle. Nach Analyse des sekretierten IL-6 wurde außerdem die Aktivität einer Ala-Pro spezifischen Endoprotease festgestellt. Auch im Falle von IFN α -2a konnte eine partielle Abspaltung des N-terminalen Ala-Pro-Dipeptids festgestellt werden. Ein Protease, welche mutmaßlich an der Prozessierung dieser Ala-Pro-Dipeptide beteiligt ist, wurde per Gendeletion identifiziert. Diese Protease wies signifikante Ähnlichkeiten mit der S. cerevisiae Ste13p auf. Zur allgemeinen Verbesserung der Ausbeute bei der Produktion heterologer Proteine wurden neue Fusionsprotein-Strategien untersucht (Manuskript II). Hierfür wurde das rekombinant cytosolisch stark exprimierte Protein Ferritin (schwere Kette, FTH1) im Hinblick auf das Potenzial als Fusionspartner für schwierig zu exprimieren Zielproteine analysiert. Ein Fragment des Parathormons (PTH) wurde gewählt, um zu untersuchen, ob die Fusion beider Proteine die Stabilität von PTH erhöht. Wir konnten zeigen, dass die Expression dieses Fusionsproteins mindestens in der Größenordnung des FTH1 ohne Fusionspartner war. Die Löslichkeit des Fusionsproteins wurde gegenüber FTH1 allein erhöht und es trat kein Abbau des PTH in Fusion mit FTH1 auf. Ectoin und 5-Hydroxyectoin gehören zur Familie der kompatiblen Solute, die hauptsächlich zur Anpassung der Zelle bei osmotischem Stress durch Anpassung des Turgors beitragen. Ein H. polymorpha-Stamm wurde entwickelt, welcher die Enzyme der 5-Hydroxyectoin-Biosynthese aus H. elongata exprimiert (Manuskript III). Die Expression der Enzyme EctB, EctA, EctC und EctD in H. polymorpha resultierte in der Produktion von 5-Hydroxyectoin im Gramm pro Liter Bereich. Es fand außerdem eine nahezu vollständige Umwandlung von Ectoin in 5-Hydroxyectoin statt. Wir konnten zeigen, dass die 5-Hydroxyectoin-Synthese durch Zugabe von Methanol während der Kultivierung beeinflusst wurde. Desweiteren wurde die 5-Hydroxyectoin-Synthese durch die Zugabe von Methionin, Lysin oder Threonin herunterreguliert, was andeutet, dass in H. polymorpha eine Feedback-regulierte Aspartatkinase existiert. T2 - Neue Ansätze der heterologen Genexpression in Hansenula polymorpha zur Protein- und Metabolitenproduktion Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:061-20130527-092604-7 PB - Institut für Molekulare Mykologie, Institut für Mikrobiologie CY - Düsseldorf ER - TY - JOUR A1 - Nehr, Sascha A1 - Franzen-Reuter, Isabelle A1 - Kucejko, Catharina T1 - New directions: Future approaches to the standardized assessment of airborne pollutants affecting environmental quality JF - Atmospheric Environment KW - Luftreinhaltung Y1 - 2017 U6 - https://doi.org/10.1016/j.atmosenv.2017.06.051 SN - 1352-2310 VL - 166 SP - 570 EP - 572 PB - Elsevier ER - TY - CHAP A1 - Steffens, Jochen A1 - Steele, Daniel A1 - Guastavino, Catherine T1 - New Insights into Soundscape Evaluations Using the Experience Sampling Method T2 - Proceedings of Euronoise 2015, 31 May - 3 June, Maastricht Y1 - 2015 UR - https://www.conforg.fr/euronoise2015/proceedings/data/articles/000111.pdf SP - 1495 EP - 1500 CY - Maastricht ER -