@misc{SoehnitzEilert2021, author = {S{\"o}hnitz, Stefanie and Eilert, Eva}, title = {Open Science - Route planen und los?}, series = {ProLibris}, volume = {26}, journal = {ProLibris}, number = {4}, doi = {10.20385/opus4-3541}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:due62-opus-35412}, pages = {152 -- 156}, year = {2021}, language = {de} } @misc{SoehnitzEilert2022, author = {S{\"o}hnitz, Stefanie and Eilert, Eva}, title = {Verwendung von Bildern in wissenschaftlichen Arbeiten}, address = {D{\"u}sseldorf}, organization = {Hochschule D{\"u}sseldorf}, doi = {10.20385/opus4-3868}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:due62-opus-38680}, pages = {4}, year = {2022}, abstract = {Abbildungen sind klassische Bestandteile einer wissenschaftlichen Arbeit. Ob Datenvisualisierung, Fotografie oder Konzeptdarstellung - die Typen von Abbildungen variieren stark. Ebenso variantenreich ist die Art und Weise, wie Abbildungen in wissenschaftliche Arbeiten eingebunden werden k{\"o}nnen. Dieses Infoblatt bietet Hilfestellungen zum Zitatrecht und Urheberrecht sowie der Nachnutzung von lizenzierten Inhalten.}, language = {de} } @inproceedings{EilertSlowig2022, author = {Eilert, Eva and Slowig, Benjamin}, title = {Handlungsfelder der F{\"o}rderlinie FDMScouts.nrw - Forschungsdatenmanagement an Hochschulen f{\"u}r angewandte Wissenschaften}, volume = {Version 1}, publisher = {Zenodo}, pages = {1}, year = {2022}, abstract = {Forschungsdatenmanagement (FDM) umfasst alle Aktivit{\"a}ten, die mit der Planung, Erhebung, Aufbereitung, Speicherung, Archivierung und Ver{\"o}ffentlichung von Forschungsdaten verbunden sind. Hierbei sind sowohl generische als auch fachspezifische Aspekte zu ber{\"u}cksichtigen; außerdem m{\"u}ssen rechtliche und technische Gesichtspunkte beachtet werden. Dies erfordert ein umfangreiches Serviceportfolio - von Information und Sensibilisierung {\"u}ber Beratung und Koordination bis hin zu IT-Infrastrukturen. Mit der F{\"o}rderlinie FDMScouts.nrw unterst{\"u}tzen das Ministerium f{\"u}r Kultur und Wissenschaft des Landes Nordrhein-Westfalen und die Digitale Hochschule NRW (DH.NRW) im Rahmen der landesweiten Digitalisierungsoffensive f{\"u}nf Hochschulverb{\"u}nde bei der nachhaltigen Verankerung des Themas FDM auf strategischer und operativer Ebene. Dabei werden sie inhaltlich und organisatorisch von der Landesinitiative fdm.nrw begleitet. Die hieraus resultierenden Impulse f{\"u}r den bedarfsgerechten Auf- und Ausbau von Kontaktstellen, Kompetenzen und Kapazit{\"a}ten zum FDM sollen {\"u}ber die Verb{\"u}nde hinaus auch anderen interessierten Hochschulen f{\"u}r angewandte Wissenschaften (HAW) und Fachhochschulen (FH) zugutekommen. Das vorliegende Poster zeigt die Handlungsfelder und die einzelnen Aktivit{\"a}ten, die im Rahmen der F{\"o}rderlinie FDMScouts.nrw im ersten F{\"o}rderjahr bereits begonnen bzw. umgesetzt wurden. Dieses Poster wurde in vergleichbarer Form auf der Jahrestagung der Digitalen Hochschule NRW am 14. und 15. September 2021 pr{\"a}sentiert.}, language = {de} } @misc{BuchClaasEilertetal.2022, author = {Buch, Susanne and Claas, Oliver and Eilert, Eva and Fliegner, Angelika and Raatz, Philip and Reiter, Martin and Schick, Elena and Slowig, Benjamin}, title = {Hochschul{\"u}bergreifende Zusammenarbeit gestalten: FDMScouts.nrw}, publisher = {Zenodo}, doi = {10.5281/zenodo.7100472}, year = {2022}, abstract = {Im Rahmen des 55. Jour Fixe FDM der Landesinitiative fdm.nrw gaben die Mitglieder der F{\"o}rderlinie FDMScouts.nrw einen Einblick in ihre Zusammenarbeit und stellten die Ergebnisse ihrer bisherigen Arbeit vor. Ziel der F{\"o}rderlinie ist es, bedarfsgerechte M{\"o}glichkeiten zu schaffen, das Thema FDM sowohl strategisch als auch operativ nachhaltig an Hochschulen f{\"u}r angewandte Wissenschaften in NRW zu verankern. 10 Hochschulen in 5 Verb{\"u}nden arbeiten seit 2020 gemeinsam an Diensten und Angeboten. Koordiniert werden die Aktivit{\"a}ten von der Landesinitiative fdm.nrw. Die Originalpr{\"a}sentation ist {\"u}ber Conceptboard einsehbar: https://app.conceptboard.com/board/c87t-okqh-6utn-74xg-n182}, language = {de} } @misc{BuchEilertFliegner2022, author = {Buch, Susanne and Eilert, Eva and Fliegner, Angelika}, title = {Forschungsdatenmanagement entlang des Datenlebenszyklus}, publisher = {Zenodo}, doi = {10.5281/zenodo.7379975}, year = {2022}, abstract = {Forschungsdatenmanagement (FDM) nimmt im Forschungsalltag der Hochschulen f{\"u}r angewandte Wissenschaften (HAW) eine zunehmende gr{\"o}ßere Rolle ein und stellt an viele Forschende bisher unbekannte Anforderungen. So gilt es ein FAIRes und nachhaltiges Datenmanagement im Sinne des Kodex „Leitlinien zur Sicherung guter wissenschaftlicher Praxis" der DFG zu betreiben - f{\"u}r sich selbst, f{\"u}r die eigene Forschungsgruppe und f{\"u}r die Forschungsgemeinschaft. In dieser Veranstaltung anl{\"a}sslich des Tags der Forschungsdaten in NRW am 15.11.2022 wurden die wesentlichen Grundz{\"u}ge des Forschungsdatenmanagements anhand des Forschungsdatenlebenszyklus n{\"a}hergebracht und Praxisbeispiele und n{\"u}tzliche Links vorgestellt.   Die Originalpr{\"a}sentation ist {\"u}ber Conceptboard einsehbar: https://app.conceptboard.com/board/pd16-f504-imy3-hrz6-gtxu}, language = {de} } @phdthesis{Eilert2013, author = {Eilert, Eva}, title = {New applications of heterologous gene expression in Hansenula polymorpha for protein and metabolite production}, publisher = {Institut f{\"u}r Molekulare Mykologie, Institut f{\"u}r Mikrobiologie}, address = {D{\"u}sseldorf}, organization = {Institut f{\"u}r Molekulare Mykologie, Institut f{\"u}r Mikrobiologie}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:061-20130527-092604-7}, year = {2013}, abstract = {The methylotrophic yeast Hansenula polymorpha has been established as a highly efficient expression system. Strong promoters derived from the methanol metabolism pathway and mitotically stable high-copy integration have made this organism very effective for heterologous protein expression. To improve the processing of secretory proteins targeted by the MFα prepro sequence, variations in the amino acid composition near the processing site of Kex2p were investigated (Manuscript I). The insertion of Ala-Pro between MFα prepro sequence and protein of interest leads to an improved processing of the signal sequence in case of interleukin-6 (IL-6) and interferon-α 2a (IFNα-2a). Modeling of the transition sequences confirmed a better exposition of the Kex2p-cleavage site in dependency of Ala-Pro insertions. Analysis of the secreted proteins revealed activity of an Ala-Pro specific endoprotease. Partial removal of the N-terminal Ala-Pro dipeptide was also detected during IFNα-2a processing. The putative protease responsible for the processing of Ala-Pro dipeptides was identified by gene deletion. Significant similarities with Saccharomyces cerevisiae Ste13p were identified. To generally improve productivity in the H. polymorpha system new fusion protein strategies were investigated (Manuscript II). The recombinantly strongly expressed cytosolic ferritin heavy chain (FTH1) was analyzed with regard to its potential as fusion partner for difficult-to-express target proteins. A fragment of the parathyroid hormone (PTH) was chosen to explore whether fusion to FTH1 enhances PTH stability. We could show that the expression level of this fusion protein was at least of the magnitude of that of FTH1 without fusion partner. The solubility of the fusion protein was increased in comparison to FTH1 alone and no degradation of PTH occurred when fused C-terminally to FTH1. Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. H. polymorpha was engineered to express the enzymes of the H. elongata 5-hydroxyectoine biosynthesis pathway (Manuscript III). Expression of the enzymes EctB, EctA, EctC and EctD in H. polymorpha resulted in production of 5-hydroxyectoine in gram per liter scale with almost full conversion of ectoine to 5-hydroxyectoine. We showed that 5-hydroxyectoine synthesis was influenced by feeding of methanol during cultivation. Furthermore, 5-hydroxyectoine synthesis was downregulated by the addition of methionine, lysine or threonine indicating the existence of a feedback regulated aspartate kinase in H. polymorpha.}, language = {en} } @misc{SuckowEilertPiontek2013, author = {Suckow, Manfred and Eilert, Eva and Piontek, Michael}, title = {Verfahren zum Herstellen eines Ziel-Proteins im Verbund mit einem heterologen Ferritin-Protein unter Verwendung von Hefe-Wirtszellen}, organization = {Artes Biotechnology GmbH}, year = {2013}, abstract = {Klassifikationen IPC C12N1/16; C12N15/81; C12R1/645; CPC C12N15/81 (EP); C12P21/02 (EP); C07K2319/01 (EP); C07K2319/35 (EP);}, language = {de} } @misc{KraemerSvetlichnyEilert2017, author = {Kraemer, Marco and Svetlichny, Vitaly and Eilert, Eva}, title = {Method for producing a fermentation product: Angemeldet durch DIREVO Industrial Biotechnology GmbH}, organization = {DIREVO Industrial Biotechnology GmbH}, year = {2017}, abstract = {Priorit{\"a}tsdaten: EP15191039A·2015-10-22; US201562245054P·2015-10-22 Bibliographische IPC: C12P7/06; C12P7/14;}, language = {en} } @misc{EilertSuckowPiontek2014, author = {Eilert, Eva and Suckow, Manfred and Piontek, Michael}, title = {A method for producing ectoine or a derivative thereof and a yeast cell for use as a host cell in such a method}, organization = {Artes Biotechnology GmbH}, year = {2014}, abstract = {Klassifikationen IPC C12N15/81; C12N15/82; C12P17/12; C12P7/42; CPC C12N15/815 (EP); C12P17/12 (EP);}, language = {en} } @article{EilertRolfHeumaieretal.2013, author = {Eilert, Eva and Rolf, Theresa and Heumaier, Andreas and Hollenberg, Cornelis P. and Piontek, Michael and Suckow, Manfred}, title = {Improved processing of secretory proteins in Hansenula polymorpha by sequence variation near the processing site of the alpha mating factor prepro sequence}, series = {Journal of biotechnology}, volume = {167}, journal = {Journal of biotechnology}, number = {2}, publisher = {Elsevier}, doi = {10.1016/j.jbiotec.2012.08.024}, pages = {94 -- 100}, year = {2013}, abstract = {The literature as well as databases are ambiguous about the exact start of human interleukin-6 (IL-6)--three possibilities for the initiation of the mature protein are described. These three variants of IL-6, different in the exact initiation of the mature protein (A28, P29, or V30), were expressed in Hansenula polymorpha using the Saccharomyces cerevisiae MFα prepro sequence instead of the homologous pre sequence. All three IL-6 variants were secreted but the processing by the Kex2 protease showed significant differences. V30-IL-6 showed correctly processed material but also a molecule species of higher molecular weight indicating incomplete processing of the MFα pro peptide. P29-IL-6 did not yield any correctly processed IL-6, instead only the unprocessed pro form was found in the culture supernatant. Only A28-IL-6 led to 100\% correctly processed material. N-terminal sequencing of this material revealed a start at V30--obviously the first two amino acids (Ala28-Pro29) have been removed by a so far unknown protease. Thus expression of both A28-IL-6 and V30-IL-6 as MFα prepro fusion proteins resulted in the very same mature V30-IL-6, however, the ratio of correctly processed molecules was significantly higher in the case of A28-IL-6. The expression of an MFα prepro-interferon α-2a (IFNα-2a) fusion protein in H. polymorpha leads to about 50\% correctly processed molecules and 50\% misprocessed forms which contain part of the pro peptide at the N-termini. The insertion of A28 and P29 of IL-6 between the pro peptide and the start of the mature IFNα-2a led to correct processing and elimination of all high molecular weight isoforms observed in earlier experiments.}, language = {en} }