TY - JOUR A1 - Kok, Maurits A1 - Huber, Florian A1 - Kalisch, Svenja-Marei A1 - Dogterom, Marileen T1 - EB3-informed dynamics of the microtubule stabilizing cap during stalled growth T2 - Biophysical Journal N2 - Microtubule stability is known to be governed by a stabilizing GTP/GDP-Pi cap, but the exact relation between growth velocity, GTP hydrolysis, and catastrophes remains unclear. We investigate the dynamics of the stabilizing cap through in vitro reconstitution of microtubule dynamics in contact with microfabricated barriers, using the plus-end binding protein GFP-EB3 as a marker for the nucleotide state of the tip. The interaction of growing microtubules with steric objects is known to slow down microtubule growth and accelerate catastrophes. We show that the lifetime distributions of stalled microtubules, as well as the corresponding lifetime distributions of freely growing microtubules, can be fully described with a simple phenomenological 1D model based on noisy microtubule growth and a single EB3-dependent hydrolysis rate. This same model is furthermore capable of explaining both the previously reported mild catastrophe dependence on microtubule growth rates and the catastrophe statistics during tubulin washout experiments. KW - Mikrotubulus Y1 - 2025 UR - https://opus4.kobv.de/opus4-hs-duesseldorf/frontdoor/index/index/docId/5316 UR - https://nbn-resolving.org/urn:nbn:de:hbz:due62-opus-53164 SN - 1542-0086 VL - 124 IS - 2 SP - 227 EP - 244 PB - Elsevier ER -