Refine
Document Type
- Conference Proceeding (4)
- Article (2)
- Part of a Book (1)
- Other (1)
Is part of the Bibliography
- yes (8)
Keywords
Institute
Synchrotron-radiation-based microtomography enables the 3D analysis of biological samples in situ beyond simple visualization, providing accurate measurements and recording of temporal data. The microtomography end station at P05 (PETRA III, Hamburg, Germany), operated by the Helmholtz Zentrum Hereon, can accommodate complex sample environments such as a load frame for mechanical testing. Herein, the strain analysis of a volumetric time series dataset of small hardwood samples is presented. For this in situ mechanical testing, the load frame is operated in tensile mode, and the biogenic material samples are subdivided into those specialized for tensile forces and those of regular anatomical structure. Digital volume correlation analysis allows for the prediction of strain development at any position in the sample. The tissue specialized for tensile strength can align dislocations formed under tension such that the deformation is stronger and more ordered. The results show how high-resolution imaging of sequential loading and the subsequent localization of strain can reveal functional morphological relationships. These methods can be extended to other biological materials and may prove to be extremely relevant for the analyses of fibrous and/or layered composite materials.
Trockenheitsgefährdung und Anpassung unterschiedlicher Fichtenpopulationen (Fichte-Trockenheit)
(2019)
Similar to a normal wave, a shock wave travels as energy through matter but it causes an abrupt raise of pressure, temperature and density. In this study the influence of shock waves on wood structure were investigated in the light of possible future industrial utilization e.g. timber impregnating treatment.
Similar to a normal wave, a shock wave travels as energy through matter but it causes an abrupt rise of pressure, temperature and density. In this study, the influence of shock waves on wood structure were investigated in the light of possible future industrial utilization e.g. timber impregnating treatment. The constructed autoclave is capable of creating shock waves either by an electrical pulse breakdown of a spark gap arrangement or by electromagnetic actuators. The penetration depths of the fir wood test samples were measured using light microscopy, SEM and micro-computed tomography. Our results showed that by varying the intensity of the incident shock wave, the distance between the shock wave generating device and the test sample, and/or the orientation of test sample in relation to the shockwave, the wood tissue structure had been heavily disintegrated or hardly affected at all. An improvement on impregnation could not be found.
A non-targeted metabolomics-based approach using liquid chromatography high-resolution mass spectrometry was used to authenticate spruce wood (Picea abies) from two geographic source areas. The two sample sites were located in Germany and only 250 km apart. In order to achieve the highest possible metabolite coverage, the spruces samples were measured with four different methods using liquid chromatography high-resolution mass spectrometry. In this way, a total of approximately 4,100 features were detected, which included non-polar, polar, and intermediate-polar metabolites. Using supervised multivariate methods, a distinction between the two sample groups could be achieved on the basis of non-polar data sets. The major metabolites contributing to differentiation were identified by MS/MS experiments and were from the following classes of compounds: ceramides, fatty acids, glycerolipids, and phytosterols. Based on the soil descriptions of the two sites, it was concluded that there is probably a close relationship between nutrient availability and the differences in concentration of the marker compounds. The results show that a metabolomics-based approach is also suitable for differentiation of origin, even if the sample sites are close to each other.