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Wood profiling by non-targeted liquid chromatography high-resolution mass spectrometry: Part 2, Detection of the geographical origin of spruce wood (Picea abies) by determination of metabolite pattern

  • A non-targeted metabolomics-based approach using liquid chromatography high-resolution mass spectrometry was used to authenticate spruce wood (Picea abies) from two geographic source areas. The two sample sites were located in Germany and only 250 km apart. In order to achieve the highest possible metabolite coverage, the spruces samples were measured with four different methods using liquid chromatography high-resolution mass spectrometry. In this way, a total of approximately 4,100 features were detected, which included non-polar, polar, and intermediate-polar metabolites. Using supervised multivariate methods, a distinction between the two sample groups could be achieved on the basis of non-polar data sets. The major metabolites contributing to differentiation were identified by MS/MS experiments and were from the following classes of compounds: ceramides, fatty acids, glycerolipids, and phytosterols. Based on the soil descriptions of the two sites, it was concluded that there is probably a close relationship between nutrientA non-targeted metabolomics-based approach using liquid chromatography high-resolution mass spectrometry was used to authenticate spruce wood (Picea abies) from two geographic source areas. The two sample sites were located in Germany and only 250 km apart. In order to achieve the highest possible metabolite coverage, the spruces samples were measured with four different methods using liquid chromatography high-resolution mass spectrometry. In this way, a total of approximately 4,100 features were detected, which included non-polar, polar, and intermediate-polar metabolites. Using supervised multivariate methods, a distinction between the two sample groups could be achieved on the basis of non-polar data sets. The major metabolites contributing to differentiation were identified by MS/MS experiments and were from the following classes of compounds: ceramides, fatty acids, glycerolipids, and phytosterols. Based on the soil descriptions of the two sites, it was concluded that there is probably a close relationship between nutrient availability and the differences in concentration of the marker compounds. The results show that a metabolomics-based approach is also suitable for differentiation of origin, even if the sample sites are close to each other.show moreshow less

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Metadaten
Author:Marina Creydt, Silke LautnerGND, Jörg Fromm, Markus Fischer
URN:urn:nbn:de:kobv:eb1-opus-4925
DOI:https://doi.org/10.1016/j.chroma.2021.462737
ISSN:1873-3778
ISSN:0021-9673
Parent Title (English):Journal of Chromatography A
Publisher:Elsevier
Document Type:Article
Language:English
Year of Completion:2022
Date of first Publication:2022/01/25
Publishing Institution:Hochschule für nachhaltige Entwicklung Eberswalde
Release Date:2023/05/15
Issue:1663
Article Number:462737
Institutions / Departments:Fachbereich Holzingenieurwesen
University Bibliography:University Bibliography
Peer-Review / Referiert
Unselbstständige Forschungsdatenpublikation
Licence (German):License LogoUrheberrechtsschutz
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