TY - JOUR A1 - Gómez Damián, Pedro A. A1 - Sperl, Jonathan I. A1 - Janich, Martin A. A1 - Khegai, Oleksandr A1 - Wiesinger, Florian A1 - Glaser, Steffen J. A1 - Haase, Axel A1 - Schwaiger, Markus A1 - Schulte, Rolf F. A1 - Menzel, Marion Irene T1 - Multisite Kinetic Modeling of 13C Metabolic MR Using [1-13C]Pyruvate JF - Radiology Research and Practice N2 - Hyperpolarized13C imaging allows real-timein vivomeasurements of metabolite levels. Quantification of metabolite conversion between [1-13C]pyruvate and downstream metabolites [1-13C]alanine, [1-13C]lactate, and [13C]bicarbonate can be achieved through kinetic modeling. Since pyruvate interacts dynamically and simultaneously with its downstream metabolites, the purpose of this work is the determination of parameter values through a multisite, dynamic model involving possible biochemical pathways present in MR spectroscopy. Kinetic modeling parameters were determined by fitting the multisite model to time-domain dynamic metabolite data. The results for different pyruvate doses were compared with those of different two-site models to evaluate the hypothesis that for identical data the uncertainty of a model and the signal-to-noise ratio determine the sensitivity in detecting small physiological differences in the target metabolism. In comparison to the two-site exchange models, the multisite model yielded metabolic conversion rates with smaller bias and smaller standard deviation, as demonstrated in simulations with different signal-to-noise ratio. Pyruvate dose effects observed previously were confirmed and quantified through metabolic conversion rate values. Parameter interdependency allowed an accurate quantification and can therefore be useful for monitoring metabolic activity in different tissues. UR - https://doi.org/10.1155/2014/871619 Y1 - 2014 UR - https://doi.org/10.1155/2014/871619 UR - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:573-41646 SN - 2090-195X VL - 2014 PB - Hindawi CY - New York ER - TY - JOUR A1 - Kubala, Eugen A1 - Muñoz-Álvarez, Kim A. A1 - Topping, Geoffrey A1 - Hundshammer, Christian A1 - Feuerecker, Benedikt A1 - Gómez, Pedro A. A1 - Pariani, Giorgio A1 - Schilling, Franz A1 - Glaser, Steffen J. A1 - Schulte, Rolf F. A1 - Menzel, Marion Irene A1 - Schwaiger, Markus T1 - Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging JF - Journal of Visualized Experiments UR - https://doi.org/10.3791/54751 Y1 - 2016 UR - https://doi.org/10.3791/54751 SN - 1940-087X VL - 2016 IS - 118 PB - MyJoVE Corporation CY - Cambridge ER - TY - CHAP A1 - Kubala, Eugen A1 - Menzel, Marion Irene A1 - Feuerecker, Benedikt A1 - Glaser, Steffen J. A1 - Schwaiger, Markus ED - Canales, Angeles T1 - Molecular Imaging T2 - Biophysical Techniques in Drug Discovery UR - https://doi.org/10.1039/9781788010016-00277 Y1 - 2017 UR - https://doi.org/10.1039/9781788010016-00277 SN - 978-1-78262-733-3 SN - 978-1-78801-286-7 SP - 277 EP - 306 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Düwel, Stephan A1 - Durst, Markus A1 - Gringeri, Concetta V. A1 - Kosanke, Yvonne A1 - Gross, Claudia A1 - Janich, Martin A. A1 - Haase, Axel A1 - Glaser, Steffen J. A1 - Schwaiger, Markus A1 - Schulte, Rolf F. A1 - Braren, Rickmer A1 - Menzel, Marion Irene T1 - Multiparametric human hepatocellular carcinoma characterization and therapy response evaluation by hyperpolarized 13C MRSI JF - NMR in Biomedicine UR - https://doi.org/10.1002/nbm.3561 Y1 - 2016 UR - https://doi.org/10.1002/nbm.3561 SN - 1099-1492 SN - 0952-3480 VL - 29 IS - 7 SP - 952 EP - 960 PB - Wiley CY - New York ER -