@article{FeuereckerDurstMichaliketal.2017, author = {Feuerecker, Benedikt and Durst, Markus and Michalik, Michael and Schneider, G{\"u}nter and Saur, Dieter and Menzel, Marion Irene and Schwaiger, Markus and Schilling, Franz}, title = {Hyperpolarized 13C Diffusion MRS of Co-Polarized Pyruvate and Fumarate to Measure Lactate Export and Necrosis}, volume = {8}, journal = {Journal of Cancer}, number = {15}, publisher = {Ivyspring International Publisher}, address = {Sydney}, issn = {1837-9664}, doi = {https://doi.org/10.7150/jca.20250}, pages = {3078 -- 3085}, year = {2017}, abstract = {Background: Non-invasive tumor characterization and monitoring are among the key goals of medical imaging. Using hyperpolarized 13C-labelled metabolic probes fast metabolic pathways can be probed in real-time, providing new opportunities for tumor characterization. In this in vitro study, we investigated whether measurement of apparent diffusion coefficient (ADC) measurements and magnetic resonance spectroscopy (MRS) of co-polarized 13C-labeled pyruvic acid and fumaric acid can non-invasively detect both necrosis and changes in lactate export, which are parameters indicative of tumor aggressiveness. Methods: 13C-labeled pyruvic acid and fumaric acid were co-polarized in a preclinical hyperpolarizer and the dissolved compounds were added to prepared samples of 8932 pancreatic cancer and MCF-7 breast carcinoma cells. Extracellular lactate concentrations and cell viability were measured in separate assays. Results: The mean ratios of the ADC values of lactate and pyruvate (ADClac/ADCpyr) between MCF-7 (0.533 ± 0.015, n = 3) and 8932 pancreatic cancer cells (0.744 ± 0.064, n = 3) showed a statistically significant difference (p = 0.048). 8932 cells had higher extracellular lactate concentrations in the extracellular medium (22.97 ± 2.53 ng/µl) compared with MCF-7 cells (7.52 ± 0.59 ng/µl; p < 0.001). Fumarate-to-malate conversion was only detectable in necrotic cells, thereby allowing clear differentiation between necrotic and viable cells. Conclusion: We provide evidence that MRS of hyperpolarized 13C-labelled pyruvic acid and fumaric acid, with their respective conversions to lactate and malate, are useful for characterization of necrosis and lactate efflux in tumor cells.}, language = {en} } @article{KubalaMunozAlvarezToppingetal.2016, author = {Kubala, Eugen and Mu{\~n}oz-{\´A}lvarez, Kim A. and Topping, Geoffrey and Hundshammer, Christian and Feuerecker, Benedikt and G{\´o}mez, Pedro A. and Pariani, Giorgio and Schilling, Franz and Glaser, Steffen J. and Schulte, Rolf F. and Menzel, Marion Irene and Schwaiger, Markus}, title = {Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging}, volume = {2016}, pages = {e54751}, journal = {Journal of Visualized Experiments}, number = {118}, publisher = {MyJoVE Corporation}, address = {Cambridge}, issn = {1940-087X}, doi = {https://doi.org/10.3791/54751}, year = {2016}, language = {en} } @article{ScholzOttoHintermairetal.2015, author = {Scholz, David Johannes and Otto, Angela M. and Hintermair, Josef and Schilling, Franz and Frank, Annette and K{\"o}llisch, Ulrich and Janich, Martin A. and Schulte, Rolf F. and Schwaiger, Markus and Haase, Axel and Menzel, Marion Irene}, title = {Parameterization of hyperpolarized 13C-bicarbonate-dissolution dynamic nuclear polarization}, volume = {28}, journal = {Magnetic Resonance Materials in Physics, Biology and Medicine}, number = {6}, publisher = {Springer}, address = {Heidelberg}, issn = {1352-8661}, doi = {https://doi.org/10.1007/s10334-015-0500-9}, pages = {591 -- 598}, year = {2015}, language = {en} }