Mechanisms and Challenges for Understanding Radiation Induced Changes in Chromatin Nanoarchitecture

  • The three-dimensional architecture of genomes acts as an additional level of mode for fundamental biological processes such as DNA damage response. In this context, nanoprobing and super-resolution microscopy are powerful methods for structural analyses of genomic targets in native chromatin of single cells at resolutions of single antibodies, proteins, histones, short DNA stretches, etc. We used multi-color chromatin nanoprobing and single molecule localization microscopy of established DNA damage and chromatin markers in 3D-conserved nuclei of different cell types exposed to various types and doses of ionizing radiation. Similarly, effect of gold nanoparticles on extent and direction of cellular radiation response was assessed. Our studies revealed the nanoarchitecture of damage foci with respect to γH2AX, Mre11 or 53BP1 and their molecular rearrangements during repair processes. Nanoscopy of genomic Alu resulted in linear-quadratic dose-effects for low to higher dose ranges and in changes of H3K9me3 distribution around Alu clusters upon radiation exposure. Preliminary results show post-irradiation time dependent changes in Alu chromatin. Moreover, gold nanoparticles incorporated into cells seem to act by an interplay of radiation enhancement and chromatin remodeling leading to altered radiosensitivity. Our results contribute to the understanding of cellular radiation responses, thereby laying the basis for improved biological dosimetry and radiotherapies in future.
  • Poster

Export metadata

Additional Services

Search Google Scholar
Metadaten
Author:Jin-Ho Lee, Elizaveta Bobkova, Theresa Gier, Martin Gote, Fanz Schmidt-kaler, Emily Brieger, Emanuel Maus, Matthias Krufczik, Robert Chojowski, Friederike Korn, Schumann Sarah, Harry Scherthan, Iva Falkova, Martin FalkORCiD, Michael HausmannORCiD, Georg HildenbrandORCiD
DOI:https://doi.org/https://doi.org/10.13140/RG.2.2.31391.20647
Parent Title (German):DRO 2018
Document Type:Other
Language:English
Year of Completion:2019
Release Date:2024/11/20
GND Keyword:Genom
Institutes:Fakultät Ingenieurwissenschaften und Informatik / Studiengänge / Medical Engineering and Data Science (Bachelor)
Research Focuses / Research Horizons:Materials / Material Testing & Sensor Technology
Reviewed:nein
Einverstanden ✔
Diese Webseite verwendet technisch erforderliche Session-Cookies. Durch die weitere Nutzung der Webseite stimmen Sie diesem zu. Unsere Datenschutzerklärung finden Sie hier.