TY - CHAP A1 - Nick, Christoph A1 - Hock, Christina A1 - Emmerich, Florian A1 - Belle, Stefan A1 - Thielemann, Christiane A1 - Asmus, Tim A1 - Loose, Thomas A1 - Wienand, Karlheinz T1 - Ultrathin gold as sensor platform for biomolecules T2 - 2015 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), Changchun, China N2 - Due to the increasing number of diabetes patients worldwide there is an enormous need for accurate, fast and someday also continuous or even closed loop monitoring of blood glucose level. More than 50 years after Clark and Lyons proposed the first glucose enzyme electrodes this concept is still widely in use today. Most concepts use the enzyme glucose oxidase (GOx) that reacts with glucose. These reactions cause a current that is proportional to the amount of glucose present at the sensor. Thus, if the sample volume is known, the blood sugar level can be measured. Although these electrodes have been in use for so long they have the disadvantage of a limited shelf time. In this work we present an enzyme free approach for glucose detection applying ultrathin gold films. According to the basic Fuchs-Sondheimer-theory and other more sophisticated models the resistivity of ultrathin metal films is dominated by scattering effects at their surface. Chemical reactions at the metallic surface are expected to change the conductivity properties and thus these changes can be used to detect molecules. This can be done by creating a self-assembled monolayer at the gold surface. When molecules such as glucose bind to the end groups of this layer the electron scattering and thus the conductivity of the film is expected to change. Ultrathin gold films with a thickness of 6 nm show the largest relative change in resistivity and are thus the preferred film thickness for this application. These gold films show a significant change in resistance when model molecules sodium sulfide and dextran are present, whereas the resistance of a platinum reference electrode does not change significantly. KW - ultrathin gold KW - biosensor KW - glucose KW - enzyme free glucose sensor KW - Biosensor KW - Dünne Schicht KW - Gold Y1 - 2015 U6 - https://doi.org/10.1109/3M-NANO.2015.7425462 ER - TY - CHAP A1 - Emmerich, Florian A1 - Thielemann, Christiane T1 - Patterning of PMMA by gold-nanoparticle initiated localized decomposition T2 - 2015 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), Changchun, China N2 - The need for manipulating matter at its smallest scale has been a hot topic in research for the last decades. As the final goal of arranging single atoms has been achieved, research focuses on two targets: firstly, improving the capabilities of existing technologies towards nanometer resolution and secondly, finding new methods for producing nanostructures in a fast and easy way. Here, we present a new maskless method for sub-micro-patterning of poly(methyl methacrylate) (PMMA) thin films. By applying atomic-force-microscope (AFM) assisted nano-xerography, electric charges were locally injected with an AFM-tip into PMMA. The resulting electrostatic patterns attracted charged gold-nanoparticles, which were selectively deposited onto the PMMA layers with lateral dimensions below 200 nm. In a second step, heat treatment at 275 °C initiated a selective decomposition of the PMMA layer, only observed in PMMA-areas covered with nanoparticles, whereas uncovered areas were not modified by the heat treatment. Analyzing the grooves with the AFM, we found that lines with a width in the sub-micrometer range to several micrometers have successfully been realized. We propose this new and promising method to manufacture nano-grooves used as masking for lift-off processes, for functionalization of underlying areas, or for micro-contact-printing. KW - Nanoparticles KW - Gold KW - Thermal stability KW - Heating KW - Electrostatics KW - Polymethylmethacrylate KW - Dünnschichttechnik Y1 - 2015 U6 - https://doi.org/10.1109/3M-NANO.2015.7425480 ER - TY - JOUR A1 - Trent, Davis A1 - Thielemann, Christiane A1 - Monici, Monica T1 - How are cell and tissue structure and function influenced by gravity and what are the gravity perception mechanisms? JF - npj Microgravity N2 - Progress in mechanobiology allowed us to better understand the important role of mechanical forces in the regulation of biological processes. Space research in the field of life sciences clearly showed that gravity plays a crucial role in biological processes. The space environment offers the unique opportunity to carry out experiments without gravity, helping us not only to understand the effects of gravitational alterations on biological systems but also the mechanisms underlying mechanoperception and cell/tissue response to mechanical and gravitational stresses. Despite the progress made so far, for future space exploration programs it is necessary to increase our knowledge on the mechanotransduction processes as well as on the molecular mechanisms underlying microgravity-induced cell and tissue alterations. This white paper reports the suggestions and recommendations of the SciSpacE Science Community for the elaboration of the section of the European Space Agency roadmap “Biology in Space and Analogue Environments” focusing on “How are cells and tissues influenced by gravity and what are the gravity perception mechanisms?” The knowledge gaps that prevent the Science Community from fully answering this question and the activities proposed to fill them are discussed. KW - Zellkultur KW - Schwere KW - Raumfahrtbiologie Y1 - 0024 UR - https://rdcu.be/dyCjL U6 - https://doi.org/10.1038/s41526-024-00357-9 VL - 2014 IS - 10 / 16 SP - 1 EP - 7 ER - TY - CHAP A1 - Nick, Christoph A1 - Thielemann, Christiane A1 - Joshi, Ravi A1 - Schneider, Jörg T1 - Niedrigtemperatur-Transfer von vertikal ausgerichteten Kohlenstoff Nanoröhren auf Mikrostrukturen T2 - Mikro-Nano-Integration - 3. GMM-Workshop, Stuttgart, Deutschland, 2011 N2 - Vertikal angeordnete Kohlenstoff Nanoröhren (engl. vertically aligned carbon nanotubes; VA-CNT) können seit einigen Jahren mit hoher Reproduzierbarkeit durch CVD-Verfahren bei 600 °C - 800 °C auf Katalysatorschichten aus Aluminium und Eisen hergestellt werden. Sie zeigen sehr gute Eigenschaften im Bereich der Feldemission, aber auch in sub-µm Vias. Allerdings sind die relativ hohen Temperaturen oft nicht prozesskompatibel und müssen vermieden werden um keine Degradation darunterliegender Strukturen in Kauf zu nehmen. Da die Reduzierung der CVD-Temperaturen beim Wachstum der VA-CNTs unter 600 °C zu hohen Defektraten führt, schlagen wir ein neues Verfahren zum Transfer der VA-CNTs bei niedrigen Temperaturen vor. In einer Kombination aus Flip-Chip- und Stempel-Technik werden hoch geordnete, mikrostrukturierte VA-CNTs auf temperaturempfindliche Substrate aus Kalk-Natron-Glas, Gold oder Kupfer bei Temperaturen von 100 °C transferiert. Die vertikale Struktur der Nanoröhren bleibt dabei erhalten. Eine leitende Verbindung kann durch ein leitfähiges, bio-kompatibles Polymer gewährleistet werden, was eine Erweiterung des Einsatzbereichs auf Life-Science Applikationen erlauben würde. KW - Kohlenstoff-Nanoröhre Y1 - 2011 ER - TY - CHAP A1 - Yilmazoglu, Oktay A1 - Al-Daffaie, Shihab A1 - Hartnagel, Hans A1 - Joshi, Ravi A1 - Yadav, Sandeep A1 - Schneider, Jörg A1 - Nick, Christoph A1 - Thielemann, Christiane T1 - CNT photocathodes based on GaAs high-frequency photoswitches T2 - 25th International Vacuum Nanoelectronics Conference, 9 - 13 July 2012, Jeju, South Korea N2 - A high-frequency photocathode based on carbon nanotube (CNT) blocks on semiisolating GaAs or low-temperature grown GaAs was fabricated and used for electron emission in a diode configuration. The CNT blocks can achieve high currents and current densities of I=0.5 mA and J=28 mA/cm 2 at E=2.1 V/μm, respectively. The photomodulation was achieved with a GaAs photoswitch electrically connected to the bottom of the CNT block emitter. This photocathode can find applications in high frequency tubes and optically driven X-ray sources. KW - Kohlenstoff-Nanoröhre Y1 - 2012 U6 - https://doi.org/10.1109/IVNC.2012.6316840 ER - TY - JOUR A1 - Emmerich, Florian A1 - Thielemann, Christiane T1 - Optimizing dimensions of unipolar Teflon-FEP piezoelectrets with micro-system-technology JF - Journal of Physics: Conference Series N2 - In this work we present a new micro-system-technology based production process for unipolar ferroelectrets. Theoretical analysis of the influence of the air-gap size on the electric field distribution as well as on the induced charge on the electrodes shows superior performance of unipolar piezoelectrets with small air-gaps. For the production of these small air-gaps we developed a new design using a photoresist thermoforming master, an integrated micro-heater and shadow masks for metallization. Unipolar piezoelectrets produced with this technology exhibit increased d33-coefficients compared to designs in previous publications. These piezoelectrets are highly preferable for energy-harvesting applications, as they promise high electric power output. KW - Mikrosystemtechnik Y1 - 2017 VL - 2018 IS - 1052 SP - 1 EP - 4 ER - TY - CHAP A1 - Mayer, Margot A1 - Arrizabalaga, Onetsine A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Human Embryonic Stem Cell Derived Neurospheres - A Novel Three Dimensional Model For Neurotoxicological Studies T2 - Frontiers N2 - At present, most neurotoxicological studies in the field of microelectrode array (MEA) technology are based on in vivo or in vitro animal models, particularly in mice or rats. These models contributed much to our knowledge about molecular and cellular mechanisms of neurotoxins affecting the central nervous system. Yet, animal models are not necessarily capable to forecasting the effectiveness of treatment in clinical trials. Therefore the development and characterization of suitable neuronal cell models derived from human cells is emerging in the field of toxicity testing. In this study, we present neurospheres (NS) as a new three dimensional (3D) neuronal cell model based on human embryonic stem cells (hESC), coupled onto microelectrode arrays (MEA). This cell-based assay serves as platform to investigate effects of neuroactive substances on network communication. In order to characterize the neurospheres’ reaction, the well-known GABA receptor antagonist bicuculline and the sodium channel blocker carbamazepine are applied. Preliminary results reveal anticipated and physiologically reasonable alterations in network activity. Thus, we have the first evidence that hESC derived NS are a promising 3D cell model for neurotoxicity testing. In further studies, this model will be used to investigate effects of ionizing radiation on network signaling. KW - human embryonic stem cells KW - neurospheres KW - MEA chips KW - 3D KW - Mikroelektrode KW - Array KW - Embryonale Stammzelle Y1 - 2016 U6 - https://doi.org/10.3389/conf.fnins.2016.93.00081 VL - 2016 IS - MEA Meeting 2016 ER - TY - JOUR A1 - Nick, Christoph A1 - Schlaak, Helmut A1 - Thielemann, Christiane T1 - Simulation and Measurement of Neuroelectrodes' Characteristics with Integrated High Aspect Ratio Nano Structures JF - AIMS Journal N2 - Improving the interface between electrodes and neurons has been the focus of research for the last decade. Neuroelectrodes should show small geometrical surface area and low impedance for measuring and high charge injection capacities for stimulation. Increasing the electrochemically active surface area by using nanoporous electrode material or by integrating nanostructures onto planar electrodes is a common approach to improve this interface. In this paper a simulation approach for neuro electrodes' characteristics with integrated high aspect ratio nano structures based on a point-contact-model is presented. The results are compared with experimental findings conducted with real nanostructured microelectrodes. In particular, effects of carbon nanotubes and gold nanowires integrated onto microelectrodes are described. Simulated and measured impedance properties are presented and its effects onto the transfer function between the neural membrane potential and the amplifier output signal are studied based on the point-contact-model. Simulations show, in good agreement with experimental results, that electrode impedances can be dramatically reduced by the integration of high aspect ratio nanostructures such as gold nanowires and carbon nanotubes. This lowers thermal noise and improves the signal-to-noise ratio for measuring electrodes. It also may increase the adhesion of cells to the substrate and thus increase measurable signal amplitudes. KW - Neuronales Netz KW - Mikroelektrode Y1 - 2015 U6 - https://doi.org/10.3934/matersci.2015.3.189 VL - 2015 IS - 2 SP - 189 EP - 202 ER - TY - JOUR A1 - Alves, Caroline L. A1 - Wissel, Lennart A1 - Capetian, Philipp A1 - Thielemann, Christiane T1 - Functional connectivity and convolutional neural networks for automatic classification of EEG data JF - Clinical Neurophysiology KW - Elektroencephalographie KW - Neuronales Netz Y1 - 2022 U6 - https://doi.org/https://doi.org/10.1016/j.clinph.2022.01.086 VL - 2022 IS - 137 SP - e37 EP - e47 ER - TY - JOUR A1 - Bouillet, Thomas A1 - Ciba, Manuel A1 - Alves, Caroline L. A1 - Rodrigues, Francisco A. A1 - Thielemann, Christiane A1 - Colin, Morvane A1 - Buée, Luc A1 - Halliez, Sophie T1 - Revisiting the involvement of tau in complex neural network remodeling: analysis of the extracellular neuronal activity in organotypic brain slice co-cultures JF - Journal of Neural Engineering N2 - Objective: Tau ablation has a protective effect in epilepsy due to inhibition of the hyperexcitability/hypersynchrony. Protection may also occur in transgenic models of Alzheimer's disease by reducing the epileptic activity and normalizing the excitation/inhibition imbalance. However, it is difficult to determine the exact functions of tau, because tau knockout (tauKO) brain networks exhibit elusive phenotypes. In this study, we aimed to further explore the physiological role of tau using brain network remodeling. Approach: The effect of tau ablation was investigated in hippocampal-entorhinal slice co-cultures during network remodeling. We recorded the spontaneous extracellular neuronal activity over two weeks in single-slice cultures and co-cultures from control and tauKO mice. We compared the burst parameters and applied concepts and analytical tools intended for the analysis of the network synchrony and connectivity. Main results: Comparison of the control and tauKO co-cultures revealed that tau ablation had an anti-synchrony effect on the hippocampal-entorhinal two-slice networks at late stages of culture, in line with the literature. Differences were also found between the single-slice and co-culture conditions, which indicated that tau ablation had differential effects at the sub-network scale. For instance, tau ablation was found to have an anti-synchrony effect on the co-cultured hippocampal slices throughout the culture, possibly due to a reduction in the excitation/inhibition ratio. Conversely, tau ablation led to increased synchrony in the entorhinal slices at early stages of the co-culture, possibly due to homogenization of the connectivity distribution. Significance: The new methodology presented here proved useful for investigating the role of tau in the remodeling of complex brain-derived neural networks. The results confirm previous findings and hypotheses concerning the effects of tau ablation on neural networks. Moreover, the results suggest, for the first time, that tau has multifaceted roles that vary in different brain sub-networks. KW - Neuronales Netz KW - Alzheimerkrankheit KW - Gehirn KW - Schnittpräparat Y1 - 2022 U6 - https://doi.org/DOI 10.1088/1741-2552/aca261 VL - 2022 IS - November SP - 1 EP - 2 ER - TY - JOUR A1 - Ciba, Manuel A1 - Bestel, Robert A1 - Nick, Christoph A1 - Ferraz de Arruda, Guilherme A1 - Peron, Thomas A1 - Henrique, Comin César A1 - da Fontoura Costa, Luciano A1 - Aparecido Rodrigues, Francisco A1 - Thielemann, Christiane T1 - Comparison of Different Spike Train Synchrony Measures Regarding Their Robustness to Erroneous Data from Bicuculline-Induced Epileptiform Activity JF - Neural Computation N2 - As synchronized activity is associated with basic brain functions and pathological states, spike train synchrony has become an important measure to analyze experimental neuronal data. Many measures of spike train synchrony have been proposed, but there is no gold standard allowing for comparison of results from different experiments. This work aims to provide guidance on which synchrony measure is best suited to quantify the effect of epileptiform-inducing substances (e.g., bicuculline, BIC) in in vitro neuronal spike train data. Spike train data from recordings are likely to suffer from erroneous spike detection, such as missed spikes (false negative) or noise (false positive). Therefore, different timescale-dependent (cross-correlation, mutual information, spike time tiling coefficient) and timescale-independent (Spike-contrast, phase synchronization (PS), A-SPIKE-synchronization, A-ISI-distance, ARI-SPIKE-distance) synchrony measures were compared in terms of their robustness to erroneous spike trains. For this purpose, erroneous spike trains were generated by randomly adding (false positive) or deleting (false negative) spikes (in silico manipulated data) from experimental data. In addition, experimental data were analyzed using different spike detection threshold factors in order to confirm the robustness of the synchrony measures. All experimental data were recorded from cortical neuronal networks on microelectrode array chips, which show epileptiform activity induced by the substance BIC. As a result of the in silico manipulated data, Spike-contrast was the only measure that was robust to false-negative as well as false-positive spikes. Analyzing the experimental data set revealed that all measures were able to capture the effect of BIC in a statistically significant way, with Spike-contrast showing the highest statistical significance even at low spike detection thresholds. In summary, we suggest using Spike contrast to complement established synchrony measures because it is timescale independent and robust to erroneous spike trains. KW - Neuronales Netz Y1 - 2020 U6 - https://doi.org/10.1162/neco_a_01277 VL - 32 IS - 3 SP - 1 EP - 25 ER - TY - JOUR A1 - Oster, Stefan A1 - Daus, Andreas A1 - Erbes, Christian A1 - Goldhammer, Michael A1 - Bochtler, Ulrich A1 - Thielemann, Christiane T1 - Long‐term electromagnetic exposure of developing neuronal networks: A flexible experimental setup JF - Bioelectromagnetics N2 - Neuronal networks in vitro are considered one of the most promising targets of research to assess potential electromagnetic field induced effects on neuronal functionality. A few exposure studies revealed there is currently no evidence of any adverse health effects caused by weak electromagnetic fields. Nevertheless, some published results are inconsistent. Particularly, doubts have been raised regarding possible athermal biological effects in the young brain during neuronal development. Therefore, we developed and characterized a flexible experimental setup based on a transverse electromagnetic waveguide, allowing controlled, reproducible exposure of developing neuronal networks in vitro. Measurement of S‐parameters confirmed very good performance of the Stripline in the band of 800–1000 MHz. Simulations suggested a flexible positioning of cell culture dishes throughout a large exposure area, as specific absorption rate values were quite independent of their position (361.7 ± 11.4 mW/kg) at 1 W, 900 MHz. During exposure, thermal drift inside cellular medium did not exceed 0.1 K. Embryonic rat cortical neurons were cultivated on microelectrode array chips to non‐invasively assess electrophysiological properties of electrogenic networks. Measurements were taken for several weeks, which attest to the experimental setup being a reliable system for long‐term studies on developing neuronal tissue. KW - RF exposure setup KW - mobile communication frequencies KW - SAR dosimetry KW - microelectrode array KW - electrophysiology KW - Neuronales Netz KW - Mikroelektrode KW - Array Y1 - 2016 U6 - https://doi.org/10.1002/bem.21974 VL - 2016 IS - 37 SP - 264 EP - 278 ER - TY - JOUR A1 - Ciba, Manuel A1 - Isomura, Takuya A1 - Jimbo, Yasuhiko A1 - Bahmer, Andreas A1 - Thielemann, Christiane T1 - Spike-contrast: A novel time scale independent and multivariate measure of spike train synchrony JF - Journal of Neuroscience Methods N2 - Background: Synchrony within neuronal networks is thought to be a fundamental feature of neuronal networks. In order to quantify synchrony between spike trains, various synchrony measures were developed. Most of them are time scale dependent and thus require the setting of an appropriate time scale. Recently, alternative methods have been developed, such as the time scale independent SPIKE-distance by Kreuz et al. New method: In this study, a novel time-scale independent spike train synchrony measure called Spike-contrast is proposed. The algorithm is based on the temporal “contrast” (activity vs. non-activity in certain temporal bins) and not only provides a single synchrony value, but also a synchrony curve as a function of the bin size. Results: For most test data sets synchrony values obtained with Spike-contrast are highly correlated with those of the SPIKE-distance (Spearman correlation value of 0.99). Correlation was lower for data containing multiple time scales (Spearman correlation value of 0.89). When analyzing large sets of data, Spike-contrast performed faster. Comparison of existing method: Spike-contrast is compared to the SPIKE-distance algorithm. The test data consisted of artificial spike trains with various levels of synchrony, including Poisson spike trains and bursts, spike trains from simulated neuronal Izhikevich networks, and bursts made of smaller bursts (sub-bursts). Conclusions: The high correlation of Spike-contrast with the established SPIKE-distance for most test data, suggests the suitability of the proposed measure. Both measures are complementary as SPIKE-distance provides a synchrony profile over time, whereas Spike-contrast provides a synchrony curve over bin size. KW - Synchrony KW - Neuronal Networks KW - Point processes KW - Time series analysis KW - Parallel spike trains KW - Neuronales Netz Y1 - 2017 U6 - https://doi.org/10.1016/j.jneumeth.2017.09.008 VL - 2018 IS - 293 SP - 136 EP - 143 ER - TY - JOUR A1 - Bestel, Robert A1 - Appali, Revathi A1 - van Rienen, Ursula A1 - Thielemann, Christiane T1 - Effect of Morphologic Features of Neurons on the Extracellular Electric Potential: A Simulation Study Using Cable Theory and Electro-Quasi-Static Equations JF - Neural Computation N2 - Microelectrode arrays serve as an indispensable tool in electro-physiological research to study the electrical activity of neural cells, enabling measurements of single cell as well as network communication analysis. Recent experimental studies have reported that the neuronal geometry has an influence on electrical signaling and extracellular recordings. However, the corresponding mechanisms are not yet fully understood and require further investigation. Allowing systematic parameter studies, computational modeling provides the opportunity to examine the underlying effects that influence extracellular potentials. In this letter, we present an in silico single cell model to analyze the effect of geometrical variability on the extracellular electric potentials. We describe finite element models of a single neuron with varying geometric complexity in three-dimensional space. The electric potential generation of the neuron is modeled using Hodgkin-Huxley equations. The signal propagation is described with electro-quasi-static equations, and results are compared with corresponding cable equation descriptions. Our results show that both the geometric dimensions and the distribution of ion channels of a neuron are critical factors that significantly influence both the amplitude and shape of extracellular potentials. KW - Mikroelektrode KW - Array Y1 - 2017 U6 - https://doi.org/10.1162/neco_a_01019 VL - 2017 IS - 29 SP - 2955 EP - 2978 ER - TY - JOUR A1 - Alves, Caroline L. A1 - Pineda, Aruane M. A1 - Roster, Kirstin A1 - Thielemann, Christiane A1 - Rodrigues, Francisco A. T1 - EEG functional connectivity and deep learning for automatic diagnosis of brain disorders: Alzheimer’s disease and schizophrenia JF - Journal of Physics: complexity N2 - Mental disorders are among the leading causes of disability worldwide. The first step in treating these conditions is to obtain an accurate diagnosis. Machine learning algorithms can provide a possible solution to this problem, as we describe in this work. We present a method for the automatic diagnosis of mental disorders based on the matrix of connections obtained from EEG time series and deep learning. We show that our approach can classify patients with Alzheimer’s disease and schizophrenia with a high level of accuracy. The comparison with the traditional cases, that use raw EEG time series, shows that our method provides the highest precision. Therefore, the application of deep neural networks on data from brain connections is a very promising method for the diagnosis of neurological disorders. KW - complex networks KW - Machine learning KW - Hirnfunktionsstörung KW - Alzheimerkrankheit KW - Schizophrenie KW - Elektroencephalographie Y1 - 2022 U6 - https://doi.org/DOI 10.1088/2632-072X/ac5f8d VL - 2022 IS - 3 SP - 1 EP - 13 ER - TY - JOUR A1 - Krstić, Nenad A1 - Jüttner, Jens A1 - Giegerich, Lars A1 - Mayer, Margot A1 - Knuth, Monika A1 - Müller, Achim A1 - Thielemann, Christiane T1 - 3D printed biosensor for continuous glucose measurement in cell cultures JF - Annals of 3D Printed Medicine KW - Biosensor KW - Zellkultur Y1 - 2023 U6 - https://doi.org/doi.org/10.1016/j.stlm.2023.100111 SN - 2666-9641 VL - 10 PB - Elsevier BV ER - TY - RPRT A1 - Mayer, Margot A1 - Müller, Benedikt A1 - Kadereit, Suzanne A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Human neurospheres on microelectrode arrays: a model to investigate ionizing radiation effects on neuronal network communication T2 - GSI SCIENTIFIC REPORT 2014 KW - Neuronales Netz KW - Mikroelektrode KW - Array KW - Ionisierende Strahlung Y1 - 2014 U6 - https://doi.org/10.15120/GR-2015-1-APPA-HEALTH-15 ER - TY - RPRT A1 - Materna, Miriam A1 - Helm, Alexander A1 - Hessel, Petra A1 - Frieß, Johannes A1 - Thielemann, Christiane A1 - Pignalosa, Diana A1 - Mäntele, Werner A1 - Durante, Marco A1 - Ritter, Sylvia T1 - First electrophysiological studies on mouse embryonic stem cell-derived cardiomyocytes KW - Herzmuskelzelle KW - Elektrophysiologie Y1 - 2012 ER - TY - CHAP A1 - Arnheiter, Frank A1 - Asmus, Tim A1 - Loose, Thomas A1 - Wienand, Karlheinz A1 - Thielemann, Christiane T1 - Metal-nanosensor for the detection of biomolecules T2 - Mikro-Nano Integration (GMM-Fachbericht 60), KW - Mikrosystemtechnik KW - Sensor Y1 - 2014 SN - 978-3-8007-3155-8 ER - TY - CHAP A1 - Emmerich, Florian A1 - Thielemann, Christiane T1 - Observation of Thermal Discharge in Nanopatterned Electrets by Kelvin-Probe-Microscopy T2 - 14th International Symposium on Elecrets, 2011, Montpellier, France KW - Elektret Y1 - 2014 SN - 978-1-4577-1023-0 SN - 2153-3253 SP - 149 EP - 150 ER - TY - CHAP A1 - Frieß, Johannes A1 - Ritter, Sylvia A1 - Daus, Andreas A1 - Layer, Paul A1 - Thielemann, Christiane A1 - Heselich, Anja T1 - Electrophysiologic and Molecular Characteristics of Cardiomyocytes after Heavy Ion Irradiation in the frame of the ESA IBER-10 Program T2 - Heavy Ion in Therapie and Space Radiation Symposium (HITSRS2013), 2013, Chiba, Japan KW - Herzmuskelzelle KW - Ionenbestrahlung KW - Schweres Ion Y1 - 2013 ER - TY - JOUR A1 - Nick, Christoph A1 - Quednau, Sebastian A1 - Sarwar, Reza A1 - Schlaak, Helmut A1 - Thielemann, Christiane T1 - High Aspect Ratio Gold Nanopillars on Microelectrodes for Neural Interfaces JF - Microsystem Technologies Media KW - nanopillars KW - Neuronales Netz KW - Mikroelektrode Y1 - 2013 UR - http://link.springer.com/article/10.1007%2Fs00542-013-1958-x SN - 0946-7076 IS - 20/10-11 SP - 1849 EP - 1857 ER - TY - CHAP A1 - Nick, Christoph A1 - Bestel, Robert A1 - Steger, Frederik A1 - Thielemann, Christiane T1 - Spike Detection, Sorting and Propagation of Cell Signals Recorded with Extracellular Microelectrodes, Engineering in Medicine and Biology Society T2 - IEMBS’13, 35th Annual International Conference of the IEEE KW - Pulsverarbeitendes neuronales Netz KW - Zellwachstum Y1 - 2013 ER - TY - JOUR A1 - Nick, Christoph A1 - Joshi, Ravi A1 - Schneider, Jörg A1 - Thielemann, Christiane T1 - Low Temperature Substrate Transfer Technique for 3D Vertically Aligned Carbon Nanotube Architectures JF - International Journal of Surface Science and Engineering KW - nanotube architectures KW - Kohlenstoff-Nanoröhre Y1 - 2012 U6 - https://doi.org/10.1504/IJSURFSE.2012.049057 VL - 6 IS - 3 SP - 246 EP - 246 ER - TY - CHAP A1 - Daus, Andreas A1 - Oster, Stefan A1 - Erbes, Christian A1 - Goldhammer, Michael A1 - Bochtler, Ulrich A1 - Thielemann, Christiane T1 - Electromagnetic exposure of neuronal networks on microelectrode arrays T2 - U.R.S.I. Landesausschuss in der Bundesrepublik Deutschland e.V., Kleinheubacher Tagung, Miltenberg, Abstracts KW - Neuronales Netz KW - Mikroelektrode KW - Array Y1 - 2013 SP - 29 EP - 30 ER -