TY - CHAP A1 - Mayer, Margot A1 - Arrizabalaga, Onetsine A1 - Schröder, Insa A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Human Embryonic Stem Cell Derived Neurospheres – 2D and 3D Cell Culture in one sample N2 - Various studies have shown that two dimensional (2D) neuronal cell cultures does not recapitulate structure and physiology of three-dimensional (3D) in vivo tissues. These findings are of paramount importance for drug screening, since the response to neurotoxicological substances may differ for 2D und 3D cell culture models. To address this topic, we present human embryonic stem cell (hESC) derived neurospheres (NS) coupled onto microelectrode array (MEA) chips as a model system that includes a 3D NS as well as an outgrowing 2D monolayer allowing direct comparison of functionality within one culture. Preliminary results revealed an enhanced functional reactions of 3D NS to GABA receptor antagonist bicuculline compared to the 2D domain. Thus we have the first evidence that hESC derived NS are a promising model system for neurotoxicity testing enabling a direct comparison between functionality of neurons grown in 2D or 3D. KW - MEA Chips KW - neurospheres KW - human embryonic stem cells KW - 3D KW - Bicuculline KW - Embryonale Stammzelle Y1 - 2018 UR - https://www.frontiersin.org/10.3389/conf.fncel.2018.38.00078/event_abstract ER - TY - CHAP A1 - Mayer, Margot A1 - Arrizabalaga, Onetsine A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Human Embryonic Stem Cell Derived Neurospheres - A Novel Three Dimensional Model For Neurotoxicological Studies T2 - Frontiers N2 - At present, most neurotoxicological studies in the field of microelectrode array (MEA) technology are based on in vivo or in vitro animal models, particularly in mice or rats. These models contributed much to our knowledge about molecular and cellular mechanisms of neurotoxins affecting the central nervous system. Yet, animal models are not necessarily capable to forecasting the effectiveness of treatment in clinical trials. Therefore the development and characterization of suitable neuronal cell models derived from human cells is emerging in the field of toxicity testing. In this study, we present neurospheres (NS) as a new three dimensional (3D) neuronal cell model based on human embryonic stem cells (hESC), coupled onto microelectrode arrays (MEA). This cell-based assay serves as platform to investigate effects of neuroactive substances on network communication. In order to characterize the neurospheres’ reaction, the well-known GABA receptor antagonist bicuculline and the sodium channel blocker carbamazepine are applied. Preliminary results reveal anticipated and physiologically reasonable alterations in network activity. Thus, we have the first evidence that hESC derived NS are a promising 3D cell model for neurotoxicity testing. In further studies, this model will be used to investigate effects of ionizing radiation on network signaling. KW - human embryonic stem cells KW - neurospheres KW - MEA chips KW - 3D KW - Mikroelektrode KW - Array KW - Embryonale Stammzelle Y1 - 2016 U6 - https://doi.org/10.3389/conf.fnins.2016.93.00081 VL - 2016 IS - MEA Meeting 2016 ER -