TY - JOUR A1 - Erenpreisa, Jekaterina A1 - Giuliani, Alessandro A1 - Yoshikawa, Kenichi A1 - Falk, Martin A1 - Hildenbrand, Georg A1 - Salmina, Kristine A1 - Freivalds, Talivaldis A1 - Vainshelbaum, Ninel A1 - Weidner, Jonas A1 - Sievers, Aaron A1 - Pilarczyk, Götz A1 - Hausmann, Michael T1 - Spatial-Temporal Genome Regulation in Stress-Response and Cell-Fate Change JF - International Journal of Molecular Sciences N2 - Complex functioning of the genome in the cell nucleus is controlled at different levels: (a) the DNA base sequence containing all relevant inherited information; (b) epigenetic pathways consisting of protein interactions and feedback loops; (c) the genome architecture and organization activating or suppressing genetic interactions between different parts of the genome. Most research so far has shed light on the puzzle pieces at these levels. This article, however, attempts an integrative approach to genome expression regulation incorporating these different layers. Under environmental stress or during cell development, differentiation towards specialized cell types, or to dysfunctional tumor, the cell nucleus seems to react as a whole through coordinated changes at all levels of control. This implies the need for a framework in which biological, chemical, and physical manifestations can serve as a basis for a coherent theory of gene self-organization. An international symposium held at the Biomedical Research and Study Center in Riga, Latvia, on 25 July 2022 addressed novel aspects of the abovementioned topic. The present article reviews the most recent results and conclusions of the state-of-the-art research in this multidisciplinary field of science, which were delivered and discussed by scholars at the Riga symposium. KW - Zellkern KW - Genom Y1 - 2023 U6 - https://doi.org/https://doi.org/10.3390/ijms24032658 SN - 1422-0067 VL - 24 IS - 3 PB - MDPI AG ER - TY - CHAP A1 - Hausmann, Michael A1 - Hildenbrand, Georg A1 - Pilarczyk, Götz T1 - Networks and Islands of Genome Nano-architecture and Their Potential Relevance for Radiation Biology BT - (A Hypothesis and Experimental Verification Hints) T2 - Results and Problems in Cell Differentiation N2 - The cell nucleus is a complex biological system in which simultaneous reactions and functions take place to keep the cell as an individualized, specialized system running well. The cell nucleus contains chromatin packed in various degrees of density and separated in volumes of chromosome territories and subchromosomal domains. Between the chromatin, however, there is enough “free” space for floating RNA, proteins, enzymes, ATPs, ions, water molecules, etc. which are trafficking by super- and supra-diffusion to the interaction points where they are required. It seems that this trafficking works somehow automatically and drives the system perfectly. After exposure to ionizing radiation causing DNA damage from single base damage up to chromatin double-strand breaks, the whole system “cell nucleus” responds, and repair processes are starting to recover the fully functional and intact system. In molecular biology, many individual epigenetic pathways of DNA damage response or repair of single and double-strand breaks are described. How these responses are embedded into the response of the system as a whole is often out of the focus of consideration. In this article, we want to follow the hypothesis of chromatin architecture’s impact on epigenetic pathways and vice versa. Based on the assumption that chromatin acts like an “aperiodic solid state within a limited volume,” functionally determined networks and local topologies (“islands”) can be defined that drive the appropriate repair process at a given damage site. Experimental results of investigations of the chromatin nano-architecture and DNA repair clusters obtained by means of single-molecule localization microscopy offer hints and perspectives that may contribute to verifying the hypothesis. KW - Zellkern KW - Genom Y1 - 2022 SN - 9783031065729 U6 - https://doi.org/https://doi.org/10.1007/978-3-031-06573-6_1 SN - 0080-1844 SP - 3 EP - 34 PB - Springer International Publishing CY - Cham ER - TY - JOUR A1 - Hausmann, Michael A1 - Neitzel, Charlotte A1 - Bobkova, Elizaveta A1 - Nagel, David A1 - Hofmann, Andreas A1 - Chramko, Tatyana A1 - Smirnova, Elena A1 - Kopecna, Olga A1 - Pagáčová, Eva A1 - Boreyko, Alla A1 - Krasavin, Evgeny A1 - Falkova, Iva A1 - Heermann, Dieter W. A1 - Pilarczyk, Götz A1 - Hildenbrand, Georg A1 - Bestvater, Felix A1 - Falk, Martin T1 - Single Molecule Localization Microscopy Analyses of DNA-Repair Foci and Clusters Detected Along Particle Damage Tracks JF - Frontiers in Physics KW - Krebszelle KW - Strahlentherapie KW - Genom KW - Zellkern Y1 - 2020 U6 - https://doi.org/https://doi.org/10.3389/fphy.2020.578662 SN - 2296-424X VL - 8 PB - Frontiers Media SA ER -