TY - CHAP A1 - Hildenbrand, Georg A1 - Paschek, Klaus A1 - Schäfer, Myriam A1 - Hausmann, Michael T1 - Cryovolcanism in the Solar System and beyond: Considerations on Energy Sources, Geological Aspects, and Astrobiological Perspectives T2 - Astronomy and Planetary Science - From Cryovolcanism to Black Holes and Galactic Evolution N2 - Volcanism based on melting rocks (silicate volcanism) is long known on Earth and has also been found on Jupiter’s moon Io. Remnants of this type of volcanism have been identified also on other bodies in the solar system. Energy sources powered by accretion and the decay of radioactive isotopes seem to be dominant mainly inside larger bodies, which have enough volume to accumulate and retain this energy in significant amounts. On the other hand, the impact of tidal forces allows even tiny bodies to melt up and pass into the stage of cryovolcanism. The dependence of tidal heating on the size of the object is minor, but the masses of and the distances to accompanying bodies as well as the inner compositions of the heated body are central factors. Even though Io as an example of a body supporting silicate volcanism is striking, the physics of tidal forces might suggest a relatively high probability for cryovolcanism. This chapter aims at considering the parameters known and objects found so far in our solar system to give insights into where in our system and other planetary systems cryovolcanism might be expected. KW - Sonnensystem KW - Vulkanismus Y1 - 2022 SN - 9781803561196 U6 - https://doi.org/https://doi.org/10.5772/intechopen.105067 PB - IntechOpen ER - TY - JOUR A1 - Hausmann, Michael A1 - Neitzel, Charlotte A1 - Bobkova, Elizaveta A1 - Nagel, David A1 - Hofmann, Andreas A1 - Chramko, Tatyana A1 - Smirnova, Elena A1 - Kopecna, Olga A1 - Pagáčová, Eva A1 - Boreyko, Alla A1 - Krasavin, Evgeny A1 - Falkova, Iva A1 - Heermann, Dieter W. A1 - Pilarczyk, Götz A1 - Hildenbrand, Georg A1 - Bestvater, Felix A1 - Falk, Martin T1 - Single Molecule Localization Microscopy Analyses of DNA-Repair Foci and Clusters Detected Along Particle Damage Tracks JF - Frontiers in Physics KW - Krebszelle KW - Strahlentherapie KW - Genom KW - Zellkern Y1 - 2020 U6 - https://doi.org/https://doi.org/10.3389/fphy.2020.578662 SN - 2296-424X VL - 8 PB - Frontiers Media SA ER - TY - JOUR A1 - Bartosova, Maria A1 - Ridinger, David A1 - Marinovic, Iva A1 - Heigwer, Jana A1 - Zhang, Conghui A1 - Levai, Eszter A1 - Westhoff, Jens H. A1 - Schaefer, Franz A1 - Terjung, Stefan A1 - Hildenbrand, Georg A1 - Krunic, Damir A1 - Bestvater, Felix A1 - Hausmann, Michael A1 - Schmitt, Claus Peter A1 - Zarogiannis, Sotirios G. T1 - An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept JF - International Journal of Molecular Sciences N2 - Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes. The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ). Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer. Human umbilical vein endothelial cells were grown on Transwell filters. Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator. Single monolayers were immunolabelled for Zonula Occludens-1 (ZO-1) and Claudin-5 (CLDN5) and used for automated immunofluorescence imaging. Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis. The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell–cell interface. At the nanoscale level, the functional and protein abundance data were accompanied by non-random increased clustering of CLDN5. Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia. KW - Endothelzelle KW - Nabelvene Y1 - 2021 U6 - https://doi.org/https://doi.org/10.3390/ijms22158178 SN - 1422-0067 VL - 22 IS - 15 PB - MDPI AG ER - TY - CHAP A1 - Hausmann, Michael A1 - Lee, Jin-Ho A1 - Sievers, Aaron A1 - Krufczik, Matthias A1 - Hildenbrand, Georg T1 - COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes T2 - The Nucleus KW - Genom Y1 - 2020 SN - 9781071607626 U6 - https://doi.org/https://doi.org/10.1007/978-1-0716-0763-3_6 SN - 1064-3745 SP - 65 EP - 77 PB - Springer US CY - New York, NY ER - TY - JOUR A1 - Dobešová, Lucie A1 - Gier, Theresa A1 - Kopecna, Olga A1 - Pagáčová, Eva A1 - Vičar, Tomáš A1 - Bestvater, Felix A1 - Toufar, Jiří A1 - Bačíková, Alena A1 - Kopel, Pavel A1 - Fedr, Radek A1 - Hildenbrand, Georg A1 - Falková, Iva A1 - Falk, Martin A1 - Hausmann, Michael T1 - Incorporation of Low Concentrations of Gold Nanoparticles: Complex Effects on Radiation Response and Fate of Cancer Cells JF - Pharmaceutics N2 - (1) Background: In oncology research, a long-standing discussion exists about pros and cons of metal nanoparticle-enhanced radiotherapy and real mechanisms behind the tumor cell response to irradiation (IR) in presence of gold nanoparticles (GNPs). A better understanding of this response is, however, necessary to develop more efficient and safety nanoparticle (NP) types designed to disturb specific processes in tumor cells. (2) Aims and Methods: We combined 3D confocal microscopy and super-resolution single molecule localization microscopy (SMLM) to analyze, at the multiscale, the early and late effects of 10 nm-GNPs on DNA double strand break (DSB) induction and repair in tumor cells exposed to different doses of photonic low-LET (linear energy transfer) radiation. The results were correlated to different aspects of short and long-term cell viability. SkBr3 breast cancer cells (selected for the highest incidence of this cancer type among all cancers in women, and because most breast tumors are treated with IR) were incubated with low concentrations of GNPs and irradiated with 60Co γ-rays or 6 MV X-rays. In numerous post-irradiation (PI) times, ranging from 0.5 to 24 h PI, the cells were spatially (3D) fixed and labeled with specific antibodies against γH2AX, 53BP1 and H3K9me3. The extent of DSB induction, multi-parametric micro- and nano-morphology of γH2AX and 53BP1 repair foci, DSB repair kinetics, persistence of unrepaired DSBs, nanoscale clustering of γH2AX and nanoscale (hetero)chromatin re-organization were measured by means of the mentioned microscopy techniques in dependence of radiation dose and GNP concentration. (3) Results: The number of γH2AX/53BP1 signals increased after IR and an additional increase was observed in GNP-treated (GNP(+)) cells compared to untreated controls. However, this phenomenon reflected slight expansion of the G2-phase cell subpopulation in irradiated GNP(+) specimens instead of enhanced DNA damage induction by GNPs. This statement is further supported by some micro- and nano-morphological parameters of γH2AX/53BP1 foci, which slightly differed for cells irradiated in absence or presence of GNPs. At the nanoscale, Ripley’s distance frequency analysis of SMLM signal coordinate matrices also revealed relaxation of heterochromatin (H3K9me3) clusters upon IR. These changes were more prominent in presence of GNPs. The slight expansion of radiosensitive G2 cells correlated with mostly insignificant but systematic decrease in post-irradiation survival of GNP(+) cells. Interestingly, low GNP concentrations accelerated DSB repair kinetics; however, the numbers of persistent γH2AX/53BP1 repair foci were slightly increased in GNP(+) cells. (4) Conclusions: Low concentrations of 10-nm GNPs enhanced the G2/M cell cycle arrest and the proportion of radiosensitive G2 cells, but not the extent of DNA damage induction. GNPs also accelerated DSB repair kinetics and slightly increased presence of unrepaired γH2AX/53BP1 foci at 24 h PI. GNP-mediated cell effects correlated with slight radiosensitization of GNP(+) specimens, significant only for the highest radiation dose tested (4 Gy). KW - Strahlentherapie KW - Krebs, Medizin KW - Tumorzelle KW - Gold KW - Nanopartikel Y1 - 2022 U6 - https://doi.org/https://doi.org/10.3390/pharmaceutics14010166 SN - 1999-4923 VL - 14 IS - 1 PB - MDPI AG ER - TY - JOUR A1 - Hausmann, Michael A1 - Falk, Martin A1 - Neitzel, Charlotte A1 - Hofmann, Andreas A1 - Biswas, Abin A1 - Gier, Theresa A1 - Falkova, Iva A1 - Heermann, Dieter W. A1 - Hildenbrand, Georg T1 - Elucidation of the Clustered Nano-Architecture of Radiation-Induced DNA Damage Sites and Surrounding Chromatin in Cancer Cells: A Single Molecule Localization Microscopy Approach JF - International Journal of Molecular Sciences N2 - In cancer therapy, the application of (fractionated) harsh radiation treatment is state of the art for many types of tumors. However, ionizing radiation is a “double-edged sword”—it can kill the tumor but can also promote the selection of radioresistant tumor cell clones or even initiate carcinogenesis in the normal irradiated tissue. Individualized radiotherapy would reduce these risks and boost the treatment, but its development requires a deep understanding of DNA damage and repair processes and the corresponding control mechanisms. DNA double strand breaks (DSBs) and their repair play a critical role in the cellular response to radiation. In previous years, it has become apparent that, beyond genetic and epigenetic determinants, the structural aspects of damaged chromatin (i.e., not only of DSBs themselves but also of the whole damage-surrounding chromatin domains) form another layer of complex DSB regulation. In the present article, we summarize the application of super-resolution single molecule localization microscopy (SMLM) for investigations of these structural aspects with emphasis on the relationship between the nano-architecture of radiation-induced repair foci (IRIFs), represented here by γH2AX foci, and their chromatin environment. Using irradiated HeLa cell cultures as an example, we show repair-dependent rearrangements of damaged chromatin and analyze the architecture of γH2AX repair clusters according to topological similarities. Although HeLa cells are known to have highly aberrant genomes, the topological similarity of γH2AX was high, indicating a functional, presumptively genome type-independent relevance of structural aspects in DSB repair. Remarkably, nano-scaled chromatin rearrangements during repair depended both on the chromatin domain type and the treatment. Based on these results, we demonstrate how the nano-architecture and topology of IRIFs and chromatin can be determined, point to the methodological relevance of SMLM, and discuss the consequences of the observed phenomena for the DSB repair network regulation or, for instance, radiation treatment outcomes. KW - Krebszelle KW - Strahlentherapie Y1 - 2021 U6 - https://doi.org/https://doi.org/10.3390/ijms22073636 SN - 1422-0067 VL - 22 IS - 7 PB - MDPI AG ER - TY - JOUR A1 - Bobkova, Elizaveta A1 - Depes, Daniel A1 - Lee, Jin-Ho A1 - Jezkova, Lucie A1 - Falkova, Iva A1 - Pagacova, Eva A1 - Kopecna, Olga A1 - Zadneprianetc, Mariia A1 - Bacikova, Alena A1 - Kulikova, Elena A1 - Smirnova, Elena A1 - Bulanova, Tatiana A1 - Boreyko, Alla A1 - Krasavin, Evgeny A1 - Wenz, Frederik A1 - Bestvater, Felix A1 - Hildenbrand, Georg A1 - Hausmann, Michael A1 - Falk, Martin T1 - Recruitment of 53BP1 Proteins for DNA Repair and Persistence of Repair Clusters Differ for Cell Types as Detected by Single Molecule Localization Microscopy JF - International Journal of Molecular Sciences N2 - DNA double stranded breaks (DSBs) are the most serious type of lesions introduced into chromatin by ionizing radiation. During DSB repair, cells recruit different proteins to the damaged sites in a manner dependent on local chromatin structure, DSB location in the nucleus, and the repair pathway entered. 53BP1 is one of the important players participating in repair pathway decision of the cell. Although many molecular biology details have been investigated, the architecture of 53BP1 repair foci and its development during the post-irradiation time, especially the period of protein recruitment, remains to be elucidated. Super-resolution light microscopy is a powerful new tool to approach such studies in 3D-conserved cell nuclei. Recently, we demonstrated the applicability of single molecule localization microscopy (SMLM) as one of these highly resolving methods for analyses of dynamic repair protein distribution and repair focus internal nano-architecture in intact cell nuclei. In the present study, we focused our investigation on 53BP1 foci in differently radio-resistant cell types, moderately radio-resistant neonatal human dermal fibroblasts (NHDF) and highly radio-resistant U87 glioblastoma cells, exposed to high-LET 15N-ion radiation. At given time points up to 24 h post irradiation with doses of 1.3 Gy and 4.0 Gy, the coordinates and spatial distribution of fluorescently tagged 53BP1 molecules was quantitatively evaluated at the resolution of 10–20 nm. Clusters of these tags were determined as sub-units of repair foci according to SMLM parameters. The formation and relaxation of such clusters was studied. The higher dose generated sufficient numbers of DNA breaks to compare the post-irradiation dynamics of 53BP1 during DSB processing for the cell types studied. A perpendicular (90°) irradiation scheme was used with the 4.0 Gy dose to achieve better separation of a relatively high number of particle tracks typically crossing each nucleus. For analyses along ion-tracks, the dose was reduced to 1.3 Gy and applied in combination with a sharp angle irradiation (10° relative to the cell plane). The results reveal a higher ratio of 53BP1 proteins recruited into SMLM defined clusters in fibroblasts as compared to U87 cells. Moreover, the speed of foci and thus cluster formation and relaxation also differed for the cell types. In both NHDF and U87 cells, a certain number of the detected and functionally relevant clusters remained persistent even 24 h post irradiation; however, the number of these clusters again varied for the cell types. Altogether, our findings indicate that repair cluster formation as determined by SMLM and the relaxation (i.e., the remaining 53BP1 tags no longer fulfill the cluster definition) is cell type dependent and may be functionally explained and correlated to cell specific radio-sensitivity. The present study demonstrates that SMLM is a highly appropriate method for investigations of spatiotemporal protein organization in cell nuclei and how it influences the cell decision for a particular repair pathway at a given DSB site. KW - Genom KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2018 U6 - https://doi.org/https://doi.org/10.3390/ijms19123713 SN - 1422-0067 VL - 19 IS - 12 PB - MDPI AG ER - TY - JOUR A1 - Hildenbrand, Georg A1 - Metzler, Philipp A1 - Pilarczyk, Götz A1 - Bobu, Vladimir A1 - Kriz, Wilhelm A1 - Hosser, Hiltraud A1 - Fleckenstein, Jens A1 - Krufczik, Matthias A1 - Bestvater, Felix A1 - Wenz, Frederik A1 - Hausmann, Michael ED - Baptista, Pedro V. T1 - Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells JF - PLOS ONE KW - Krebs, Medizin KW - Brustkrebs KW - Strahlentherapie Y1 - 2018 U6 - https://doi.org/https://doi.org/10.1371/journal.pone.0190183 SN - 1932-6203 VL - 13 IS - 1 PB - Public Library of Science (PLoS) ER - TY - JOUR A1 - Moser, Felipe A1 - Hildenbrand, Georg A1 - Müller, Patrick A1 - Al Saroori, Alexander A1 - Biswas, Abin A1 - Bach, Margund A1 - Wenz, Frederik A1 - Cremer, Christoph A1 - Burger, Nina A1 - Veldwijk, Marlon R. A1 - Hausmann, Michael T1 - Cellular Uptake of Gold Nanoparticles and Their Behavior as Labels for Localization Microscopy JF - Biophysical Journal KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2016 U6 - https://doi.org/https://doi.org/10.1016/j.bpj.2016.01.004 SN - 0006-3495 VL - 110 IS - 4 SP - 947 EP - 953 PB - Elsevier BV ER - TY - JOUR A1 - Bosiek, Katharina A1 - Hausmann, Michael A1 - Hildenbrand, Georg T1 - Perspectives on Comets, Comet-like Asteroids, and Their Predisposition to Provide an Environment That Is Friendly to Life JF - Astrobiology KW - Komet KW - Planetoid Y1 - 2016 U6 - https://doi.org/https://doi.org/10.1089/ast.2015.1354 SN - 1531-1074 VL - 16 IS - 4 SP - 311 EP - 323 PB - Mary Ann Liebert Inc ER - TY - JOUR A1 - Hausmann, Michael A1 - Winkler, Ralph A1 - Hildenbrand, Georg A1 - Finsterle, Jutta A1 - Weisel, Andrea A1 - Rapp, Alexander A1 - Schmitt, Eberhard A1 - Janz, Siegfried A1 - Cremer, Christoph T1 - COMBO-FISH: specific labeling of nondenatured chromatin targets by computer-selected DNA oligonucleotide probe combinations JF - BioTechniques KW - Genom Y1 - 2003 U6 - https://doi.org/https://doi.org/10.2144/03353rr03 SN - 0736-6205 VL - 35 IS - 3 SP - 564 EP - 577 PB - Informa UK Limited ER - TY - GEN A1 - Lee, Jin-Ho A1 - Bobkova, Elizaveta A1 - Gier, Theresa A1 - Gote, Martin A1 - Schmidt-kaler, Fanz A1 - Brieger, Emily A1 - Maus, Emanuel A1 - Krufczik, Matthias A1 - Chojowski, Robert A1 - Korn, Friederike A1 - Sarah, Schumann A1 - Scherthan, Harry A1 - Falkova, Iva A1 - Falk, Martin A1 - Hausmann, Michael A1 - Hildenbrand, Georg T1 - Mechanisms and Challenges for Understanding Radiation Induced Changes in Chromatin Nanoarchitecture T2 - DRO 2018 N2 - The three-dimensional architecture of genomes acts as an additional level of mode for fundamental biological processes such as DNA damage response. In this context, nanoprobing and super-resolution microscopy are powerful methods for structural analyses of genomic targets in native chromatin of single cells at resolutions of single antibodies, proteins, histones, short DNA stretches, etc. We used multi-color chromatin nanoprobing and single molecule localization microscopy of established DNA damage and chromatin markers in 3D-conserved nuclei of different cell types exposed to various types and doses of ionizing radiation. Similarly, effect of gold nanoparticles on extent and direction of cellular radiation response was assessed. Our studies revealed the nanoarchitecture of damage foci with respect to γH2AX, Mre11 or 53BP1 and their molecular rearrangements during repair processes. Nanoscopy of genomic Alu resulted in linear-quadratic dose-effects for low to higher dose ranges and in changes of H3K9me3 distribution around Alu clusters upon radiation exposure. Preliminary results show post-irradiation time dependent changes in Alu chromatin. Moreover, gold nanoparticles incorporated into cells seem to act by an interplay of radiation enhancement and chromatin remodeling leading to altered radiosensitivity. Our results contribute to the understanding of cellular radiation responses, thereby laying the basis for improved biological dosimetry and radiotherapies in future. N2 - Poster KW - Genom Y1 - 2019 U6 - https://doi.org/https://doi.org/10.13140/RG.2.2.31391.20647 ER - TY - JOUR A1 - Ngwa, Wilfred A1 - Boateng, Francis A1 - Kumar, Rajiv A1 - Irvine, Darrell J. A1 - Formenti, Silvia A1 - Ngoma, Twalib A1 - Herskind, Carsten A1 - Veldwijk, Marlon R. A1 - Hildenbrand, Georg A1 - Hausmann, Michael A1 - Wenz, Frederik A1 - Hesser, Jürgen T1 - Smart Radiation Therapy Biomaterials JF - International Journal of Radiation Oncology*Biology*Physics KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2017 U6 - https://doi.org/https://doi.org/10.1016/j.ijrobp.2016.10.034 SN - 0360-3016 VL - 97 IS - 3 SP - 624 EP - 637 PB - Elsevier BV ER - TY - JOUR A1 - Wiech, Thorsten A1 - Stein, Stefan A1 - Lachenmaier, Victoria A1 - Schmitt, Eberhard A1 - Schwarz-Finsterle, Jutta A1 - Wiech, Elisabeth A1 - Hildenbrand, Georg A1 - Werner, Martin A1 - Hausmann, Michael T1 - Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium JF - European Biophysics Journal KW - Genom KW - Krebs, Medizin Y1 - 2009 U6 - https://doi.org/https://doi.org/10.1007/s00249-009-0474-5 SN - 0175-7571 VL - 38 IS - 6 SP - 793 EP - 806 PB - Springer Science and Business Media LLC ER - TY - JOUR A1 - Müller, Patrick A1 - Lemmermann, Niels A. A1 - Kaufmann, Rainer A1 - Gunkel, Manuel A1 - Paech, Daniel A1 - Hildenbrand, Georg A1 - Holtappels, Rafaela A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Spatial distribution and structural arrangement of a murine cytomegalovirus glycoprotein detected by SPDM localization microscopy JF - Histochemistry and Cell Biology KW - Fluoreszenzmikroskopie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1007/s00418-014-1185-2 SN - 0948-6143 VL - 142 IS - 1 SP - 61 EP - 67 PB - Springer Science and Business Media LLC ER - TY - JOUR A1 - Hildenbrand, Georg A1 - Rapp, Alexander A1 - Spöri, Udo A1 - Wagner, Christian A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy JF - Biophysical Journal KW - Genom KW - Zellkern KW - Fluoreszenzmikroskopie Y1 - 2005 U6 - https://doi.org/https://doi.org/10.1529/biophysj.104.056796 SN - 0006-3495 VL - 88 IS - 6 SP - 4312 EP - 4318 PB - Elsevier BV ER - TY - JOUR A1 - Kaufmann, Rainer A1 - Müller, P A1 - Hildenbrand, Georg A1 - Hausmann, Michael A1 - Cremer, Christoph T1 - Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy BT - ANALYSIS OF HER2/neu MEMBRANE PROTEIN CLUSTERS JF - Journal of Microscopy KW - Krebs, Medizin KW - Brustkrebs Y1 - 2010 U6 - https://doi.org/https://doi.org/10.1111/j.1365-2818.2010.03436.x SN - 0022-2720 VL - 242 IS - 1 SP - 46 EP - 54 PB - Wiley ER - TY - JOUR A1 - Burger, Nina A1 - Biswas, Abin A1 - Barzan, Daniel A1 - Kirchner, Anne A1 - Hosser, Hiltraud A1 - Hausmann, Michael A1 - Hildenbrand, Georg A1 - Herskind, Carsten A1 - Wenz, Frederik A1 - Veldwijk, Marlon R. T1 - A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells JF - Nanomedicine: Nanotechnology, Biology and Medicine KW - Gold KW - Nanopartikel Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1016/j.nano.2014.03.011 SN - 1549-9634 VL - 10 IS - 6 SP - 1365 EP - 1373 PB - Elsevier BV ER - TY - JOUR A1 - Falk, Martin A1 - Hausmann, Michael A1 - Lukasova, Emilie A1 - Biswas, Abin A1 - Hildenbrand, Georg A1 - Davidkova, Marie A1 - Krasavin, Evgeny A1 - Kleibl, Zdenek A1 - Falkova, Iva A1 - Jezkova, Lucie A1 - Stefancikova, Lenka A1 - Sevcik, Jan A1 - Hofer, Michal A1 - Bacikova, Alena A1 - Matula, Pavel A1 - Boreyko, Alla A1 - Vachelova, Jana A1 - Michaelidesova, Anna A1 - Kozubek, Stanislav T1 - Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: PART A-Radiomics JF - Critical Reviews in Eukaryotic Gene Expression KW - Radiologie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1615/CritRevEukaryotGeneExpr.2014010313 SN - 1045-4403 VL - 24 IS - 3 SP - 205 EP - 223 PB - Begell House ER - TY - CHAP A1 - Hausmann, Michael A1 - Pilarczyk, Götz A1 - Maus, Emanuel A1 - Hesser, Jürgen A1 - Hildenbrand, Georg T1 - Super-resolution microscopy of nanogold-labelling T2 - Nanoparticle Enhanced Radiation Therapy KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2020 SN - 9780750323963 U6 - https://doi.org/https://doi.org/10.1088/978-0-7503-2396-3ch11 SP - 11 EP - 1 PB - IOP Publishing ER - TY - CHAP A1 - Hausmann, Michael A1 - Lee, Jin-Ho A1 - Hildenbrand, Georg T1 - 3D DNA FISH for analyses of chromatin-nuclear architecture T2 - Epigenetics Methods KW - Genom Y1 - 2020 SN - 9780128194140 U6 - https://doi.org/https://doi.org/10.1016/B978-0-12-819414-0.00020-3 SP - 399 EP - 418 PB - Elsevier ER -