TY - CHAP A1 - Ritter, Sylvia A1 - Durante, Marco A1 - Helm, Alexander A1 - Steger, Frederik A1 - Daus, Andreas A1 - Frieß, Johannes T1 - Assessment of cardiotoxicity of heavy ions in vitro: generation and electrophysiology of cardiomyocytes T2 - 39th COSPAR Scientific Assembly, Mysore, India KW - Herzmuskelzelle KW - Kardiotoxizität Y1 - 2014 ER - TY - THES A1 - Frieß, Johannes T1 - Einfluss ionisierender Strahlung auf die elektrophysiologischen Eigenschaften kardialer Zellen N2 - Die zentrale Fragestellung der Strahlenbiologie behandelt die Wirkung von ionisierender Strahlung auf biologische Systeme. Zwar sind die bei Exposition mit ionisierender Strahlung entstehenden Gesundheitsrisiken bekannt, dennoch sind die Strahlungseffekte auf das Herz immer noch nicht sehr gut verstanden. Dabei ist die Exposition mit hohen Dosen ionisierender Strahlung ein anerkannter Risikofaktor in Bezug auf kardiovaskuläre Erkrankungen. KW - Elektrophysiologie KW - Herzmuskelzelle Y1 - 2016 ER - TY - JOUR A1 - Heselich, Anja A1 - Frieß, Johannes A1 - Ritter, Sylvia A1 - Benz, Naja A1 - Layer, Paul A1 - Thielemann, Christiane T1 - High LET radiation shows no major cellular and functional effects on primary cardiomyocytes in vitro JF - Life Sciences in Space Research N2 - It is well known that ionizing radiation causes adverse effects on various mammalian tissues. However, there is little information on the biological effects of heavy ion radiation on the heart. In order to fill this gap, we systematically examined DNA-damage induction and repair, as well as proliferation and apoptosis in avian cardiomyocyte cultures irradiated with heavy ions such as titanium and iron, relevant for manned space-flight, and carbon ions, as used for radiotherapy. Further, and to our knowledge for the first time, we analyzed the effect of heavy ion radiation on the electrophysiology of primary cardiomyocytes derived from chicken embryos using the non-invasive microelectrode array (MEA) technology. As electrophysiological endpoints beat rate and field action potential duration were analyzed. The cultures clearly exhibited the capacity to repair induced DNA damage almost completely within 24 h, even at doses of 7 Gy, and almost completely recovered from radiation-induced changes in proliferative behavior. Interestingly, no significant effects on apoptosis could be detected. Especially the functionality of primary cardiac cells exhibited a surprisingly high robustness against heavy ion radiation, even at doses of up to 7 Gy. In contrast to our previous study with X-rays the beat rate remained more or less unaffected after heavy ion radiation, independently of beam quality. The only change we could observe was an increase of the field action potential duration of up to 30% after titanium irradiation, diminishing within the following three days. This potentially pathological observation may be an indication that heavy ion irradiation at high doses could bear a long-term risk for cardiovascular disease induction. KW - Cardiomyocytes KW - Heavy ion irradiation KW - Space radiation KW - Electrophysiology KW - MEA-technology KW - Zellkultur KW - Herzmuskelzelle Y1 - 2018 U6 - https://doi.org/10.1016/j.lssr.2018.01.001 VL - 2018 IS - 16 SP - 93 EP - 100 ER - TY - RPRT A1 - Materna, Miriam A1 - Helm, Alexander A1 - Hessel, Petra A1 - Frieß, Johannes A1 - Thielemann, Christiane A1 - Pignalosa, Diana A1 - Mäntele, Werner A1 - Durante, Marco A1 - Ritter, Sylvia T1 - First electrophysiological studies on mouse embryonic stem cell-derived cardiomyocytes KW - Herzmuskelzelle KW - Elektrophysiologie Y1 - 2012 ER - TY - CHAP A1 - Frieß, Johannes A1 - Ritter, Sylvia A1 - Daus, Andreas A1 - Layer, Paul A1 - Thielemann, Christiane A1 - Heselich, Anja T1 - Electrophysiologic and Molecular Characteristics of Cardiomyocytes after Heavy Ion Irradiation in the frame of the ESA IBER-10 Program T2 - Heavy Ion in Therapie and Space Radiation Symposium (HITSRS2013), 2013, Chiba, Japan KW - Herzmuskelzelle KW - Ionenbestrahlung KW - Schweres Ion Y1 - 2013 ER - TY - CHAP A1 - Steger, Frederik A1 - Frieß, Johannes A1 - Daus, Andreas A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Analysis of electrophysiological characteristics of cardiomyocytes following radiation exposure T2 - 8th International Meeting on Substrate-Integrated Microelectrode Arrays KW - Herzmuskelzelle KW - Strahlenbelastung Y1 - 2014 U6 - https://doi.org/10.13140/RG.2.1.4153.0400 SN - 2194-5519 SP - 153 ER - TY - CHAP A1 - Thielemann, Christiane A1 - Ritter, Sylvia A1 - Layer, Paul A1 - Frieß, Johannes A1 - Daus, Andreas A1 - Steger, Frederik A1 - Heselich, Anja T1 - Cellular effects of space radiation with relevance to cardiovascular diseases T2 - Life in Space for Life on Earth KW - Kardiovaskuläre Krankheit KW - Ionisierende Strahlung Y1 - 2014 N1 - IBER-10 SP - 560 ER - TY - RPRT A1 - Helm, Alexander A1 - Frank, Simon A1 - Frieß, Johannes A1 - Thielemann, Christiane A1 - Durante, Marco A1 - Ritter, Sylvia T1 - Purified cultures of mouse embryonic stem cell-derived cardiomyocytes of electrophysiological studies with ionizing radiation KW - Herzmuskelzelle KW - Ionisierende Strahlung Y1 - 2013 ER - TY - JOUR A1 - Frieß, Johannes A1 - Heselich, Anja A1 - Ritter, Sylvia A1 - Haber, Angelina A1 - Kaiser, Nicole A1 - Layer, Paul A1 - Thielemann, Christiane T1 - Electrophysiologic and cellular characteristics of cardiomyocytes after X-ray irradiation JF - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis KW - Herzmuskelzelle KW - Röntgenbestrahlung Y1 - 2015 VL - 2015 IS - 777 SP - 1 EP - 10 ER - TY - RPRT A1 - Frieß, Johannes A1 - Heselich, Anja A1 - Ritter, Sylvia A1 - Layer, Paul A1 - Thielemann, Christiane T1 - Effect of X-rays and titanium ions on cardiomyocyte cultures KW - Herzmuskelzelle KW - Röntgenbestrahlung Y1 - 2013 ER - TY - RPRT A1 - Frieß, Johannes A1 - Heselich, Anja A1 - Ritter, Sylvia A1 - Daus, Andreas A1 - Layer, Paul A1 - Thielemann, Christiane T1 - Electrophysiological Effects of Heavy Ion Irradiation on Cardiomyocytes KW - Herzmuskelzelle KW - Elektrophysiologie Y1 - 2012 ER - TY - CHAP A1 - Frieß, Johannes A1 - Heselich, Anja A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Electrophysiological characteristics of cardiomyocytes after heavy ion exposure T2 - Jahrestagung der Gesellschaft für biologische Strahlenforschung KW - Herzmuskelzelle Y1 - 2013 CY - Darmstadt, Deutschland ER - TY - CHAP A1 - Thielemann, Christiane A1 - Ritter, Sylvia A1 - Layer, Paul A1 - Frieß, Johannes A1 - Daus, Andreas A1 - Steger, Frederik A1 - Heselich, Anja T1 - IBER10 - Cellular effects of space radiation with relevance to cardiovascular diseases T2 - European Space Agency - Life in Space for Life on Earth KW - Kosmische Strahlung KW - Kardiovaskuläre Krankheit Y1 - 2012 CY - Aberdeen, Schottland, UK ER - TY - RPRT A1 - Steger, Frederik A1 - Ritter, Sylvia A1 - Daus, Andreas A1 - Frieß, Johannes A1 - Thielemann, Christiane T1 - Analysis of electrophysiological characteristics of cardiomyocytes following radiation exposure KW - Herzmuskelzelle KW - Strahlenbelastung Y1 - 2011 ER - TY - RPRT A1 - Helm, Alexander A1 - Frank, Simon A1 - Frieß, Johannes A1 - Thielemann, Christiane A1 - Durante, Marco A1 - Ritter, Sylvia T1 - Electrophysiological response of mouse embryonic stem cell-derived cardiomyocytes after X-ray and C-ion exposure T2 - GSI SCIENTIFIC REPORT 2014 KW - Embryonale Stammzelle KW - Herzmuskelzelle Y1 - 2014 U6 - https://doi.org/10.15120/GR-2015-1-APPA-HEALTH-21 ER - TY - RPRT A1 - Frieß, Johannes A1 - Heselich, Anja A1 - Ritter, Sylvia A1 - Layer, Paul A1 - Thielemann, Christiane T1 - Combined effects of ionizing radiation and cardio-active drugs on human iPSC-derived cardiomyocytes T2 - GSI SCIENTIFIC REPORT 2014 KW - Herzmuskelzelle KW - Ionisierende Strahlung Y1 - 2014 U6 - https://doi.org/10.15120/GR-2015-1-APPA-HEALTH-37 ER -