TY - JOUR A1 - Bosiek, Katharina A1 - Hausmann, Michael A1 - Hildenbrand, Georg T1 - Perspectives on Comets, Comet-like Asteroids, and Their Predisposition to Provide an Environment That Is Friendly to Life JF - Astrobiology KW - Komet KW - Planetoid Y1 - 2016 U6 - https://doi.org/https://doi.org/10.1089/ast.2015.1354 SN - 1531-1074 VL - 16 IS - 4 SP - 311 EP - 323 PB - Mary Ann Liebert Inc ER - TY - JOUR A1 - Hausmann, Michael A1 - Winkler, Ralph A1 - Hildenbrand, Georg A1 - Finsterle, Jutta A1 - Weisel, Andrea A1 - Rapp, Alexander A1 - Schmitt, Eberhard A1 - Janz, Siegfried A1 - Cremer, Christoph T1 - COMBO-FISH: specific labeling of nondenatured chromatin targets by computer-selected DNA oligonucleotide probe combinations JF - BioTechniques KW - Genom Y1 - 2003 U6 - https://doi.org/https://doi.org/10.2144/03353rr03 SN - 0736-6205 VL - 35 IS - 3 SP - 564 EP - 577 PB - Informa UK Limited ER - TY - GEN A1 - Lee, Jin-Ho A1 - Bobkova, Elizaveta A1 - Gier, Theresa A1 - Gote, Martin A1 - Schmidt-kaler, Fanz A1 - Brieger, Emily A1 - Maus, Emanuel A1 - Krufczik, Matthias A1 - Chojowski, Robert A1 - Korn, Friederike A1 - Sarah, Schumann A1 - Scherthan, Harry A1 - Falkova, Iva A1 - Falk, Martin A1 - Hausmann, Michael A1 - Hildenbrand, Georg T1 - Mechanisms and Challenges for Understanding Radiation Induced Changes in Chromatin Nanoarchitecture T2 - DRO 2018 N2 - The three-dimensional architecture of genomes acts as an additional level of mode for fundamental biological processes such as DNA damage response. In this context, nanoprobing and super-resolution microscopy are powerful methods for structural analyses of genomic targets in native chromatin of single cells at resolutions of single antibodies, proteins, histones, short DNA stretches, etc. We used multi-color chromatin nanoprobing and single molecule localization microscopy of established DNA damage and chromatin markers in 3D-conserved nuclei of different cell types exposed to various types and doses of ionizing radiation. Similarly, effect of gold nanoparticles on extent and direction of cellular radiation response was assessed. Our studies revealed the nanoarchitecture of damage foci with respect to γH2AX, Mre11 or 53BP1 and their molecular rearrangements during repair processes. Nanoscopy of genomic Alu resulted in linear-quadratic dose-effects for low to higher dose ranges and in changes of H3K9me3 distribution around Alu clusters upon radiation exposure. Preliminary results show post-irradiation time dependent changes in Alu chromatin. Moreover, gold nanoparticles incorporated into cells seem to act by an interplay of radiation enhancement and chromatin remodeling leading to altered radiosensitivity. Our results contribute to the understanding of cellular radiation responses, thereby laying the basis for improved biological dosimetry and radiotherapies in future. N2 - Poster KW - Genom Y1 - 2019 U6 - https://doi.org/https://doi.org/10.13140/RG.2.2.31391.20647 ER - TY - JOUR A1 - Ngwa, Wilfred A1 - Boateng, Francis A1 - Kumar, Rajiv A1 - Irvine, Darrell J. A1 - Formenti, Silvia A1 - Ngoma, Twalib A1 - Herskind, Carsten A1 - Veldwijk, Marlon R. A1 - Hildenbrand, Georg A1 - Hausmann, Michael A1 - Wenz, Frederik A1 - Hesser, Jürgen T1 - Smart Radiation Therapy Biomaterials JF - International Journal of Radiation Oncology*Biology*Physics KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2017 U6 - https://doi.org/https://doi.org/10.1016/j.ijrobp.2016.10.034 SN - 0360-3016 VL - 97 IS - 3 SP - 624 EP - 637 PB - Elsevier BV ER - TY - JOUR A1 - Wiech, Thorsten A1 - Stein, Stefan A1 - Lachenmaier, Victoria A1 - Schmitt, Eberhard A1 - Schwarz-Finsterle, Jutta A1 - Wiech, Elisabeth A1 - Hildenbrand, Georg A1 - Werner, Martin A1 - Hausmann, Michael T1 - Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium JF - European Biophysics Journal KW - Genom KW - Krebs, Medizin Y1 - 2009 U6 - https://doi.org/https://doi.org/10.1007/s00249-009-0474-5 SN - 0175-7571 VL - 38 IS - 6 SP - 793 EP - 806 PB - Springer Science and Business Media LLC ER - TY - JOUR A1 - Müller, Patrick A1 - Lemmermann, Niels A. A1 - Kaufmann, Rainer A1 - Gunkel, Manuel A1 - Paech, Daniel A1 - Hildenbrand, Georg A1 - Holtappels, Rafaela A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Spatial distribution and structural arrangement of a murine cytomegalovirus glycoprotein detected by SPDM localization microscopy JF - Histochemistry and Cell Biology KW - Fluoreszenzmikroskopie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1007/s00418-014-1185-2 SN - 0948-6143 VL - 142 IS - 1 SP - 61 EP - 67 PB - Springer Science and Business Media LLC ER - TY - JOUR A1 - Hildenbrand, Georg A1 - Rapp, Alexander A1 - Spöri, Udo A1 - Wagner, Christian A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy JF - Biophysical Journal KW - Genom KW - Zellkern KW - Fluoreszenzmikroskopie Y1 - 2005 U6 - https://doi.org/https://doi.org/10.1529/biophysj.104.056796 SN - 0006-3495 VL - 88 IS - 6 SP - 4312 EP - 4318 PB - Elsevier BV ER - TY - JOUR A1 - Kaufmann, Rainer A1 - Müller, P A1 - Hildenbrand, Georg A1 - Hausmann, Michael A1 - Cremer, Christoph T1 - Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy BT - ANALYSIS OF HER2/neu MEMBRANE PROTEIN CLUSTERS JF - Journal of Microscopy KW - Krebs, Medizin KW - Brustkrebs Y1 - 2010 U6 - https://doi.org/https://doi.org/10.1111/j.1365-2818.2010.03436.x SN - 0022-2720 VL - 242 IS - 1 SP - 46 EP - 54 PB - Wiley ER - TY - JOUR A1 - Burger, Nina A1 - Biswas, Abin A1 - Barzan, Daniel A1 - Kirchner, Anne A1 - Hosser, Hiltraud A1 - Hausmann, Michael A1 - Hildenbrand, Georg A1 - Herskind, Carsten A1 - Wenz, Frederik A1 - Veldwijk, Marlon R. T1 - A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells JF - Nanomedicine: Nanotechnology, Biology and Medicine KW - Gold KW - Nanopartikel Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1016/j.nano.2014.03.011 SN - 1549-9634 VL - 10 IS - 6 SP - 1365 EP - 1373 PB - Elsevier BV ER - TY - JOUR A1 - Falk, Martin A1 - Hausmann, Michael A1 - Lukasova, Emilie A1 - Biswas, Abin A1 - Hildenbrand, Georg A1 - Davidkova, Marie A1 - Krasavin, Evgeny A1 - Kleibl, Zdenek A1 - Falkova, Iva A1 - Jezkova, Lucie A1 - Stefancikova, Lenka A1 - Sevcik, Jan A1 - Hofer, Michal A1 - Bacikova, Alena A1 - Matula, Pavel A1 - Boreyko, Alla A1 - Vachelova, Jana A1 - Michaelidesova, Anna A1 - Kozubek, Stanislav T1 - Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: PART A-Radiomics JF - Critical Reviews in Eukaryotic Gene Expression KW - Radiologie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1615/CritRevEukaryotGeneExpr.2014010313 SN - 1045-4403 VL - 24 IS - 3 SP - 205 EP - 223 PB - Begell House ER - TY - CHAP A1 - Hausmann, Michael A1 - Pilarczyk, Götz A1 - Maus, Emanuel A1 - Hesser, Jürgen A1 - Hildenbrand, Georg T1 - Super-resolution microscopy of nanogold-labelling T2 - Nanoparticle Enhanced Radiation Therapy KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2020 SN - 9780750323963 U6 - https://doi.org/https://doi.org/10.1088/978-0-7503-2396-3ch11 SP - 11 EP - 1 PB - IOP Publishing ER - TY - CHAP A1 - Hausmann, Michael A1 - Lee, Jin-Ho A1 - Hildenbrand, Georg T1 - 3D DNA FISH for analyses of chromatin-nuclear architecture T2 - Epigenetics Methods KW - Genom Y1 - 2020 SN - 9780128194140 U6 - https://doi.org/https://doi.org/10.1016/B978-0-12-819414-0.00020-3 SP - 399 EP - 418 PB - Elsevier ER -