TY - JOUR A1 - Pagáčová, Eva A1 - Štefančíková, Lenka A1 - Schmidt-Kaler, Franz A1 - Hildenbrand, Georg A1 - Vičar, Tomáš A1 - Depeš, Daniel A1 - Lee, Jin-Ho A1 - Bestvater, Felix A1 - Lacombe, Sandrine A1 - Porcel, Erika A1 - Roux, Stéphane A1 - Wenz, Frederik A1 - Kopecna, Olga A1 - Falková, Iva A1 - Hausmann, Michael A1 - Falk, Martin T1 - Challenges and Contradictions of Metal Nano-Particle Applications for Radio-Sensitivity Enhancement in Cancer Therapy JF - International Journal of Molecular Sciences N2 - From the very beginnings of radiotherapy, a crucial question persists with how to target the radiation effectiveness into the tumor while preserving surrounding tissues as undamaged as possible. One promising approach is to selectively pre-sensitize tumor cells by metallic nanoparticles. However, though the “physics” behind nanoparticle-mediated radio-interaction has been well elaborated, practical applications in medicine remain challenging and often disappointing because of limited knowledge on biological mechanisms leading to cell damage enhancement and eventually cell death. In the present study, we analyzed the influence of different nanoparticle materials (platinum (Pt), and gold (Au)), cancer cell types (HeLa, U87, and SKBr3), and doses (up to 4 Gy) of low-Linear Energy Transfer (LET) ionizing radiation (γ- and X-rays) on the extent, complexity and reparability of radiation-induced γH2AX + 53BP1 foci, the markers of double stand breaks (DSBs). Firstly, we sensitively compared the focus presence in nuclei during a long period of time post-irradiation (24 h) in spatially (three-dimensionally, 3D) fixed cells incubated and non-incubated with Pt nanoparticles by means of high-resolution immunofluorescence confocal microscopy. The data were compared with our preliminary results obtained for Au nanoparticles and recently published results for gadolinium (Gd) nanoparticles of approximately the same size (2–3 nm). Next, we introduced a novel super-resolution approach—single molecule localization microscopy (SMLM)—to study the internal structure of the repair foci. In these experiments, 10 nm Au nanoparticles were used that could be also visualized by SMLM. Altogether, the data show that different nanoparticles may or may not enhance radiation damage to DNA, so multi-parameter effects have to be considered to better interpret the radiosensitization. Based on these findings, we discussed on conclusions and contradictions related to the effectiveness and presumptive mechanisms of the cell radiosensitization by nanoparticles. We also demonstrate that SMLM offers new perspectives to study internal structures of repair foci with the goal to better evaluate potential differences in DNA damage patterns. KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2019 U6 - https://doi.org/https://doi.org/10.3390/ijms20030588 SN - 1422-0067 VL - 20 IS - 3 PB - MDPI AG ER - TY - JOUR A1 - Franz, Daniel A1 - Yang, Yongting A1 - Michel, Luis A1 - Esen, Cemal A1 - Hellmann, Ralf T1 - Evaluation of an ultrashort pulsed laser robot system for flexible and large-area micromachining JF - Journal of Laser Applications N2 - We report for the first time on the realization, characterization and application of an ultrashort pulsed laser robot system for flexible and large-area 2D and 3D laser micromachining with 6 articulated axes. To characterize the dynamic positioning of the laser beam during and after axes movement, CMOS image sensors were integrated into the beam path. A method introduced for the alignment of the optical axes allows a reduction of the deviations in laser beam positioning to less than 141.8 ± 92.9 μm within a 110° rotation range of axis 4. In addition, a high laser beam positioning repeatability of less than 102.2 μm is demonstrated over a total period of 14 h for a movement of axis 5 within a range of 0° to 90°. Initial laser cutting, laser structuring and laser marking applications on automotive dashboards and glass substrates are presented for flexible and large area 2D and 3D manufacturing. By applying a special laser cutting strategy for processing AF 32 eco thin glass, high cutting quality is achieved with a taper of up to 96.3% without the generation of cracks, demonstrating the innovative potential of the high-precision laser robot system. Nonetheless, different identified inherent influences of each axis 1–5 during robot axis movement demand for an innovative beam stabilization concept to achieve high precision in laser beam positioning. KW - Ultrakurzzeitlaser KW - Industrieroboter Y1 - 2023 N1 - Proceedings of the International Congress of Applications of Lasers & Electro-Optics (ICALEO 2023) VL - 35 IS - 4 SP - 042057 EP - 042065 ER - TY - JOUR A1 - Franz, Daniel A1 - Schuster, David A1 - Schwarz, Simon A1 - Rung, Stefan A1 - Esen, Cemal A1 - Hellmann, Ralf T1 - Fabrication and analysis of through-glass vias for glass-based electronic packaging using an ultrashort pulsed laser JF - Optics and Lasers in Engineering KW - Ultrakurzzeitlaser KW - Laserbohren Y1 - 2025 U6 - https://doi.org/10.1016/j.optlaseng.2025.109106 SN - 0143-8166 VL - 193 PB - Elsevier BV ER - TY - JOUR A1 - Franz, Daniel A1 - Yang, Yongting A1 - Esen, Cemal A1 - Hellmann, Ralf ED - Esen, Cemal T1 - Flexible 2D Laser Cutting of Ultra-Thin Glass Using an Ultrashort Pulsed Laser Robotic System JF - Journal of Laser Micro/Nanoengineering KW - Laserschneiden KW - Ultrakurzzeitlaser Y1 - 2026 U6 - https://doi.org/10.2961/jlmn.2026.01.2004 SN - 1880-0688 VL - 21 IS - 1 PB - Japan Laser Processing Society ER -