TY - JOUR A1 - Solov’yov, Andrey V. A1 - Verkhovtsev, Alexey V. A1 - Mason, Nigel J. A1 - Amos, Richard A. A1 - Bald, Ilko A1 - Baldacchino, Gérard A1 - Dromey, Brendan A1 - Falk, Martin A1 - Fedor, Juraj A1 - Gerhards, Luca A1 - Hausmann, Michael A1 - Hildenbrand, Georg A1 - Hrabovský, Miloš A1 - Kadlec, Stanislav A1 - Kočišek, Jaroslav A1 - Lépine, Franck A1 - Ming, Siyi A1 - Nisbet, Andrew A1 - Ricketts, Kate A1 - Sala, Leo A1 - Schlathölter, Thomas A1 - Wheatley, Andrew E. H. A1 - Solov’yov, Ilia A. T1 - Condensed Matter Systems Exposed to Radiation: Multiscale Theory, Simulations, and Experiment JF - Chemical Reviews N2 - This roadmap reviews the new, highly interdisciplinary research field studying the behavior of condensed matter systems exposed to radiation. The Review highlights several recent advances in the field and provides a roadmap for the development of the field over the next decade. Condensed matter systems exposed to radiation can be inorganic, organic, or biological, finite or infinite, composed of different molecular species or materials, exist in different phases, and operate under different thermodynamic conditions. Many of the key phenomena related to the behavior of irradiated systems are very similar and can be understood based on the same fundamental theoretical principles and computational approaches. The multiscale nature of such phenomena requires the quantitative description of the radiation-induced effects occurring at different spatial and temporal scales, ranging from the atomic to the macroscopic, and the interlinks between such descriptions. The multiscale nature of the effects and the similarity of their manifestation in systems of different origins necessarily bring together different disciplines, such as physics, chemistry, biology, materials science, nanoscience, and biomedical research, demonstrating the numerous interlinks and commonalities between them. This research field is highly relevant to many novel and emerging technologies and medical applications. KW - Kondensierte Materie KW - Strahlung Y1 - 2024 U6 - https://doi.org/https://doi.org/10.1021/acs.chemrev.3c00902 SN - 0009-2665 VL - 124 IS - 13 SP - 8014 EP - 8129 PB - American Chemical Society (ACS) ER - TY - JOUR A1 - Hausmann, Michael A1 - Falk, Martin A1 - Neitzel, Charlotte A1 - Hofmann, Andreas A1 - Biswas, Abin A1 - Gier, Theresa A1 - Falkova, Iva A1 - Heermann, Dieter W. A1 - Hildenbrand, Georg T1 - Elucidation of the Clustered Nano-Architecture of Radiation-Induced DNA Damage Sites and Surrounding Chromatin in Cancer Cells: A Single Molecule Localization Microscopy Approach JF - International Journal of Molecular Sciences N2 - In cancer therapy, the application of (fractionated) harsh radiation treatment is state of the art for many types of tumors. However, ionizing radiation is a “double-edged sword”—it can kill the tumor but can also promote the selection of radioresistant tumor cell clones or even initiate carcinogenesis in the normal irradiated tissue. Individualized radiotherapy would reduce these risks and boost the treatment, but its development requires a deep understanding of DNA damage and repair processes and the corresponding control mechanisms. DNA double strand breaks (DSBs) and their repair play a critical role in the cellular response to radiation. In previous years, it has become apparent that, beyond genetic and epigenetic determinants, the structural aspects of damaged chromatin (i.e., not only of DSBs themselves but also of the whole damage-surrounding chromatin domains) form another layer of complex DSB regulation. In the present article, we summarize the application of super-resolution single molecule localization microscopy (SMLM) for investigations of these structural aspects with emphasis on the relationship between the nano-architecture of radiation-induced repair foci (IRIFs), represented here by γH2AX foci, and their chromatin environment. Using irradiated HeLa cell cultures as an example, we show repair-dependent rearrangements of damaged chromatin and analyze the architecture of γH2AX repair clusters according to topological similarities. Although HeLa cells are known to have highly aberrant genomes, the topological similarity of γH2AX was high, indicating a functional, presumptively genome type-independent relevance of structural aspects in DSB repair. Remarkably, nano-scaled chromatin rearrangements during repair depended both on the chromatin domain type and the treatment. Based on these results, we demonstrate how the nano-architecture and topology of IRIFs and chromatin can be determined, point to the methodological relevance of SMLM, and discuss the consequences of the observed phenomena for the DSB repair network regulation or, for instance, radiation treatment outcomes. KW - Krebszelle KW - Strahlentherapie Y1 - 2021 U6 - https://doi.org/https://doi.org/10.3390/ijms22073636 SN - 1422-0067 VL - 22 IS - 7 PB - MDPI AG ER -