TY - CHAP A1 - Ritter, Sylvia A1 - Durante, Marco A1 - Helm, Alexander A1 - Steger, Frederik A1 - Daus, Andreas A1 - Frieß, Johannes T1 - Assessment of cardiotoxicity of heavy ions in vitro: generation and electrophysiology of cardiomyocytes T2 - 39th COSPAR Scientific Assembly, Mysore, India KW - Herzmuskelzelle KW - Kardiotoxizität Y1 - 2014 ER - TY - JOUR A1 - Daus, Andreas T1 - Leben auf dem Mikrochip – Biosensoren auf der Basis von Mikroelektroden-Arrays JF - Labor & More KW - Biosensoren KW - Biosensor KW - Mikroelektrode KW - Array Y1 - 2012 VL - 2012 IS - 8 SP - 20 EP - 24 ER - TY - THES A1 - Daus, Andreas T1 - Zellbasierte Biosensoren – Hybride Systeme aus dreidimensionalen in vitro Netzwerken und Mikroelektroden Arrays N2 - Mit der Entwicklung hybrider Strukturen aus Mikroelektroden Arrays und dreidimensionalen Sphäroiden wurde in dieser Arbeit ein funktionaler, deskriptiver Ansatz der Biosensorik adressiert. Während biochemische Sensoren ausschließlich analytische Informationen über die Wechselwirkung von Liganden und Rezeptoren liefern, erlauben zellbasierte Biosensoren eine physiologische Beschreibung biologischer Systeme in nativen bzw. chemisch oder physikalisch provozierten Zuständen. Dies impliziert für Anwendungen der Pharmakologie, Toxikologie und Grundlagenforschung hohes Potenzial, evoziert jedoch eine multidisziplinäre Aufgabe. Es werden zum einen in vitro Systeme benötigt, die den Ansprüchen eines physiologisch repräsentativen Abbilds eines Organismus genügen. Zum anderen sind technische Systeme erforderlich, die Zellreaktionen qualitativ und quantitativ verwertbar machen. KW - Array KW - Biosensor Y1 - 2013 ER - TY - JOUR A1 - Samhaber, Robert A1 - Schottdorf, Manuel A1 - El Hady, Ahmed A1 - Bröking, Kai A1 - Daus, Andreas A1 - Thielemann, Christiane A1 - Stühmer, Walter A1 - Wolf, Fred T1 - Growing neuronal islands on multi-electrode arrays using an accurate positioning-μCP device JF - Journal of Neuroscience Methods N2 - Background: Multi-electrode arrays (MEAs) allow non-invasive multi-unit recording in-vitro from cultured neuronal networks. For sufficient neuronal growth and adhesion on such MEAs, substrate preparation is required. Plating of dissociated neurons on a uniformly prepared MEA's surface results in the formation of spatially extended random networks with substantial inter-sample variability. Such cultures are not optimally suited to study the relationship between defined structure and dynamics in neuronal networks. To overcome these shortcomings, neurons can be cultured with pre-defined topology by spatially structured surface modification. Spatially structuring a MEA surface accurately and reproducibly with the equipment of a typical cell-culture laboratory is challenging. New method: In this paper, we present a novel approach utilizing micro-contact printing (μCP) combined with a custom-made device to accurately position patterns on MEAs with high precision. We call this technique AP-μCP (accurate positioning micro-contact printing). Comparison with existing methods: Other approaches presented in the literature using μCP for patterning either relied on facilities or techniques not readily available in a standard cell culture laboratory, or they did not specify means of precise pattern positioning. Conclusion: Here we present a relatively simple device for reproducible and precise patterning in a standard cell-culture laboratory setting. The patterned neuronal islands on MEAs provide a basis for high throughput electrophysiology to study the dynamics of single neurons and neuronal networks. KW - MEA KW - Patterning KW - μCP KW - Mehrfachelektrode KW - Neuronales Netz Y1 - 2016 U6 - https://doi.org/10.1016/j.jneumeth.2015.09.022 VL - 2016 IS - 257 SP - 194 EP - 203 ER - TY - JOUR A1 - Nick, Christoph A1 - Daus, Andreas A1 - Bestel, Robert A1 - Goldhammer, Michael A1 - Steger, Frederik A1 - Thielemann, Christiane T1 - DrCell - a software tool for the analysis of cell signals recorded with extracellular microelectrodes JF - Signal processing: an international journal (SPIJ) KW - Mikroelektrode Y1 - 2014 UR - http://www.cscjournals.org/manuscript/Journals/SPIJ/Volume7/Issue2/SPIJ-226.pdf VL - 7 IS - 2 SP - 96 EP - 109 ER - TY - JOUR A1 - Oster, Stefan A1 - Daus, Andreas A1 - Erbes, Christian A1 - Goldhammer, Michael A1 - Bochtler, Ulrich A1 - Thielemann, Christiane T1 - Long‐term electromagnetic exposure of developing neuronal networks: A flexible experimental setup JF - Bioelectromagnetics N2 - Neuronal networks in vitro are considered one of the most promising targets of research to assess potential electromagnetic field induced effects on neuronal functionality. A few exposure studies revealed there is currently no evidence of any adverse health effects caused by weak electromagnetic fields. Nevertheless, some published results are inconsistent. Particularly, doubts have been raised regarding possible athermal biological effects in the young brain during neuronal development. Therefore, we developed and characterized a flexible experimental setup based on a transverse electromagnetic waveguide, allowing controlled, reproducible exposure of developing neuronal networks in vitro. Measurement of S‐parameters confirmed very good performance of the Stripline in the band of 800–1000 MHz. Simulations suggested a flexible positioning of cell culture dishes throughout a large exposure area, as specific absorption rate values were quite independent of their position (361.7 ± 11.4 mW/kg) at 1 W, 900 MHz. During exposure, thermal drift inside cellular medium did not exceed 0.1 K. Embryonic rat cortical neurons were cultivated on microelectrode array chips to non‐invasively assess electrophysiological properties of electrogenic networks. Measurements were taken for several weeks, which attest to the experimental setup being a reliable system for long‐term studies on developing neuronal tissue. KW - RF exposure setup KW - mobile communication frequencies KW - SAR dosimetry KW - microelectrode array KW - electrophysiology KW - Neuronales Netz KW - Mikroelektrode KW - Array Y1 - 2016 U6 - https://doi.org/10.1002/bem.21974 VL - 2016 IS - 37 SP - 264 EP - 278 ER - TY - CHAP A1 - Frieß, Johannes A1 - Ritter, Sylvia A1 - Daus, Andreas A1 - Layer, Paul A1 - Thielemann, Christiane A1 - Heselich, Anja T1 - Electrophysiologic and Molecular Characteristics of Cardiomyocytes after Heavy Ion Irradiation in the frame of the ESA IBER-10 Program T2 - Heavy Ion in Therapie and Space Radiation Symposium (HITSRS2013), 2013, Chiba, Japan KW - Herzmuskelzelle KW - Ionenbestrahlung KW - Schweres Ion Y1 - 2013 ER - TY - CHAP A1 - Daus, Andreas A1 - Oster, Stefan A1 - Erbes, Christian A1 - Goldhammer, Michael A1 - Bochtler, Ulrich A1 - Thielemann, Christiane T1 - Electromagnetic exposure of neuronal networks on microelectrode arrays T2 - U.R.S.I. Landesausschuss in der Bundesrepublik Deutschland e.V., Kleinheubacher Tagung, Miltenberg, Abstracts KW - Neuronales Netz KW - Mikroelektrode KW - Array Y1 - 2013 SP - 29 EP - 30 ER - TY - CHAP A1 - Oster, Stefan A1 - Daus, Andreas A1 - Goldhammer, Michael A1 - Bochtler, Ulrich A1 - Thielemann, Christiane T1 - A Flexible RF Exposure Setup for Long-term Electrophysiological Investigations on Biological Samples in Vitro T2 - Progress In Electromagnetics Research Symposium Abstracts, Stockholm, Schweden KW - Neuronales Netz KW - Array KW - Mikroelektrode Y1 - 2013 ER - TY - JOUR A1 - Bestel, Robert A1 - Daus, Andreas A1 - Thielemann, Christiane T1 - A novel automated spike sorting algorithm with adaptable feature extraction JF - Journal of Neuroscience Methods KW - spike sorting KW - Sortierverfahren Y1 - 2012 U6 - https://doi.org/10.1016/j.jneumeth.2012.08.015 VL - 211 IS - 1 SP - 168 EP - 178 ER - TY - CHAP A1 - Goldhammer, Michael A1 - Daus, Andreas A1 - Thielemann, Christiane A1 - Bochtler, Ulrich T1 - Flexible measurement setup for electromagnetic exposition of biological samples T2 - BioEM 2009, Davos, Switzerland KW - Elektromagnetische Strahlung KW - Messung Y1 - 2014 ER - TY - JOUR A1 - Daus, Andreas A1 - Layer, Paul A1 - Thielemann, Christiane T1 - A spheroid-based biosensor for the label-free detection of drug-induced field potential alterations JF - Sensors and actuators B: Chemical KW - Sensors KW - Actuators KW - Biosensor Y1 - 2012 U6 - https://doi.org/10.1016/j.snb.2012.02.011 VL - 165 IS - 1 SP - 53 EP - 58 ER - TY - CHAP A1 - Daus, Andreas A1 - Goldhammer, Michael A1 - Bochtler, Ulrich A1 - Thielemann, Christiane T1 - Electromagnetic exposition of neuronal networks in vitro T2 - Radiofrequency fields and health KW - Neuronales Netz Y1 - 2014 ER - TY - CHAP A1 - Daus, Andreas A1 - Hasenkopf, Matthias A1 - Thielemann, Christiane T1 - Novel 3D cell culture systems for electromagnetic exposure studies T2 - BioEM 2009, Davos, Switzerland KW - Zellkultur Y1 - 2009 ER - TY - CHAP A1 - Steger, Frederik A1 - Frieß, Johannes A1 - Daus, Andreas A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Analysis of electrophysiological characteristics of cardiomyocytes following radiation exposure T2 - 8th International Meeting on Substrate-Integrated Microelectrode Arrays KW - Herzmuskelzelle KW - Strahlenbelastung Y1 - 2014 U6 - https://doi.org/10.13140/RG.2.1.4153.0400 SN - 2194-5519 SP - 153 ER - TY - CHAP A1 - Daus, Andreas A1 - Goldhammer, Michael A1 - Thielemann, Christiane T1 - Using three-dimensional cell culture systems on Microelectrode arrays for biosensing applications T2 - 7th international Meeting on subtrate-intergrated microelectrode arrays, Reutlingen KW - Array KW - Mikroelektrode KW - Biosensor Y1 - 2014 SN - 978-3-938345-08-5 SP - 314 EP - 315 ER - TY - JOUR A1 - Daus, Andreas A1 - Goldhammer, Michael A1 - Layer, Paul A1 - Thielemann, Christiane T1 - Electromagnetic exposure of scaffold-free three-dimensional cell culture systems JF - Bioelectromagnetics KW - Zellkultur KW - Herzmuskelzelle Y1 - 2014 U6 - https://doi.org/10.1002/bem.20649 SN - 1521-186X VL - 32 IS - 5 SP - 351 EP - 359 ER - TY - CHAP A1 - Thielemann, Christiane A1 - Ritter, Sylvia A1 - Layer, Paul A1 - Frieß, Johannes A1 - Daus, Andreas A1 - Steger, Frederik A1 - Heselich, Anja T1 - Cellular effects of space radiation with relevance to cardiovascular diseases T2 - Life in Space for Life on Earth KW - Kardiovaskuläre Krankheit KW - Ionisierende Strahlung Y1 - 2014 N1 - IBER-10 SP - 560 ER - TY - CHAP A1 - Daus, Andreas A1 - Bestel, Robert A1 - Thielemann, Christiane T1 - Spike Detection, Refinement And Interpretation Of Recordings In Three-Dimensional In Vitro Systems T2 - World Congress of Regenerative Medicine & Stem Cells 2011, Beijing, China KW - Neuronales Netz Y1 - 2014 ER - TY - CHAP A1 - Daus, Andreas A1 - Goldhammer, Michael A1 - Bochtler, Ulrich A1 - Thielemann, Christiane T1 - A new approach for the investigation of long-term effects of RF radiation on cells T2 - 28th Progress In Electromagnetics Research Symposium 2010, Cambridge KW - Hochfrequenz KW - Strahlung Y1 - 2014 ER -