TY - JOUR A1 - Hausmann, Michael A1 - Ilić, Nataša A1 - Pilarczyk, Götz A1 - Lee, Jin-Ho A1 - Logeswaran, Abiramy A1 - Borroni, Aurora A1 - Krufczik, Matthias A1 - Theda, Franziska A1 - Waltrich, Nadine A1 - Bestvater, Felix A1 - Hildenbrand, Georg A1 - Cremer, Christoph A1 - Blank, Michael T1 - Challenges for Super-Resolution Localization Microscopy and Biomolecular Fluorescent Nano-Probing in Cancer Research JF - International Journal of Molecular Sciences N2 - Understanding molecular interactions and regulatory mechanisms in tumor initiation, progression, and treatment response are key requirements towards advanced cancer diagnosis and novel treatment procedures in personalized medicine. Beyond decoding the gene expression, malfunctioning and cancer-related epigenetic pathways, investigations of the spatial receptor arrangements in membranes and genome organization in cell nuclei, on the nano-scale, contribute to elucidating complex molecular mechanisms in cells and tissues. By these means, the correlation between cell function and spatial organization of molecules or molecular complexes can be studied, with respect to carcinogenesis, tumor sensitivity or tumor resistance to anticancer therapies, like radiation or antibody treatment. Here, we present several new applications for bio-molecular nano-probes and super-resolution, laser fluorescence localization microscopy and their potential in life sciences, especially in biomedical and cancer research. By means of a tool-box of fluorescent antibodies, green fluorescent protein (GFP) tagging, or specific oligonucleotides, we present tumor relevant re-arrangements of Erb-receptors in membranes, spatial organization of Smad specific ubiquitin protein ligase 2 (Smurf2) in the cytosol, tumor cell characteristic heterochromatin organization, and molecular re-arrangements induced by radiation or antibody treatment. The main purpose of this article is to demonstrate how nano-scaled distance measurements between bio-molecules, tagged by appropriate nano-probes, can be applied to elucidate structures and conformations of molecular complexes which are characteristic of tumorigenesis and treatment responses. These applications open new avenues towards a better interpretation of the spatial organization and treatment responses of functionally relevant molecules, at the single cell level, in normal and cancer cells, offering new potentials for individualized medicine. KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2017 U6 - https://doi.org/https://doi.org/10.3390/ijms18102066 SN - 1422-0067 VL - 18 IS - 10 PB - MDPI AG ER - TY - JOUR A1 - Hausmann, Michael A1 - Neitzel, Charlotte A1 - Bobkova, Elizaveta A1 - Nagel, David A1 - Hofmann, Andreas A1 - Chramko, Tatyana A1 - Smirnova, Elena A1 - Kopecna, Olga A1 - Pagáčová, Eva A1 - Boreyko, Alla A1 - Krasavin, Evgeny A1 - Falkova, Iva A1 - Heermann, Dieter W. A1 - Pilarczyk, Götz A1 - Hildenbrand, Georg A1 - Bestvater, Felix A1 - Falk, Martin T1 - Single Molecule Localization Microscopy Analyses of DNA-Repair Foci and Clusters Detected Along Particle Damage Tracks JF - Frontiers in Physics KW - Krebszelle KW - Strahlentherapie KW - Genom KW - Zellkern Y1 - 2020 U6 - https://doi.org/https://doi.org/10.3389/fphy.2020.578662 SN - 2296-424X VL - 8 PB - Frontiers Media SA ER - TY - JOUR A1 - Hildenbrand, Georg A1 - Metzler, Philipp A1 - Pilarczyk, Götz A1 - Bobu, Vladimir A1 - Kriz, Wilhelm A1 - Hosser, Hiltraud A1 - Fleckenstein, Jens A1 - Krufczik, Matthias A1 - Bestvater, Felix A1 - Wenz, Frederik A1 - Hausmann, Michael ED - Baptista, Pedro V. T1 - Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells JF - PLOS ONE KW - Krebs, Medizin KW - Brustkrebs KW - Strahlentherapie Y1 - 2018 U6 - https://doi.org/https://doi.org/10.1371/journal.pone.0190183 SN - 1932-6203 VL - 13 IS - 1 PB - Public Library of Science (PLoS) ER -