TY - JOUR A1 - Schäfer, Myriam A1 - Hildenbrand, Georg A1 - Hausmann, Michael T1 - Impact of Gold Nanoparticles and Ionizing Radiation on Whole Chromatin Organization as Detected by Single-Molecule Localization Microscopy JF - International Journal of Molecular Sciences N2 - In radiation tumor therapy, irradiation, on one hand, should cause cell death to the tumor. On the other hand, the surrounding non-tumor tissue should be maintained unaffected. Therefore, methods of local dose enhancements are highly interesting. Gold nanoparticles, which are preferentially uptaken by very-fast-proliferating tumor cells, may enhance damaging. However, the results in the literature obtained from cell culture and animal tissue experiments are very contradictory, i.e., only some experiments reveal increased cell killing but others do not. Thus, a better understanding of cellular mechanisms is required. Using the breast cancer cell model SkBr3, the effects of gold nanoparticles in combination with ionizing radiation on chromatin network organization were investigated by Single-Molecule Localization Microscopy (SMLM) and applications of mathematical topology calculations (e.g., Persistent Homology, Principal Component Analysis, etc.). The data reveal a dose and nanoparticle dependent re-organization of chromatin, although colony forming assays do not show a significant reduction of cell survival after the application of gold nanoparticles to the cells. In addition, the spatial organization of γH2AX clusters was elucidated, and characteristic changes were obtained depending on dose and gold nanoparticle application. The results indicate a complex response of ALU-related chromatin and heterochromatin organization correlating to ionizing radiation and gold nanoparticle incorporation. Such complex whole chromatin re-organization is usually associated with changes in genome function and supports the hypothesis that, with the application of gold nanoparticles, not only is DNA damage increasing but also the efficiency of DNA repair may be increased. The understanding of complex chromatin responses might help to improve the gold nanoparticle efficiency in radiation treatment. KW - Krebs, Medizin KW - Strahlentherapie Y1 - 2024 U6 - https://doi.org/https://doi.org/10.3390/ijms252312843 SN - 1422-0067 VL - 25 IS - 23 PB - MDPI AG ER - TY - JOUR A1 - Failla, Antonio Virgillo A1 - Albrecht, Benno A1 - Spöri, U. A1 - Schweitzer, A. A1 - Kroll, A. A1 - Hildenbrand, Georg A1 - Bach, M. A1 - Cremer, Christoph T1 - Nanostructure Analysis Using Spatially Modulated Illumination Microscopy JF - Complexus N2 - For an improved understanding of cellular processes, it is highly desirable to develop light optical methods for the analysis of biological nanostructures and their dynamics in the interior of three-dimensionally (3D) conserved cells. Here, important structural parameters to be considered are the topology, i.e. the mutual positions and distances, as well as the sizes of the constituting subunits. This has become possible by the development of a novel method of far-field light fluorescence microscopy, spatially modulated illumination (SMI) microscopy. Using this approach, axial distances between fluorescence-labeled targets can be measured with an accuracy close to 1 nm; their sizes can be determined down to a few tens of nanometers. This approach can be extended to the determination of 3D positions and mutual 3D distances and sizes of any number of small objects/subunits that can be discriminated due to their spectral signatures. Consequently, the new approach allows an ‘in situ nanostructure elucidation, until now regarded to be beyond the possibilities of far-field light microscopy. Application examples discussed are: colocalization/nanosizing and topological analysis of large protein-protein complexes, of nucleic acid-protein complexes (such as transcription factories), or of the highly complex DNA-protein nanostructures of which active/ inactive gene regions in the eukaryotic cell nucleus are constituted. KW - Fluoreszenzmikroskopie Y1 - 2003 U6 - https://doi.org/https://doi.org/10.1159/000070464 SN - 1424-8492 VL - 1 IS - 2 SP - 77 EP - 88 PB - S. Karger AG ER - TY - JOUR A1 - Hildenbrand, Georg A1 - Rapp, Alexander A1 - Spöri, Udo A1 - Wagner, Christian A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy JF - Biophysical Journal KW - Genom KW - Zellkern KW - Fluoreszenzmikroskopie Y1 - 2005 U6 - https://doi.org/https://doi.org/10.1529/biophysj.104.056796 SN - 0006-3495 VL - 88 IS - 6 SP - 4312 EP - 4318 PB - Elsevier BV ER - TY - JOUR A1 - Wagner, Christian A1 - Hildenbrand, Georg A1 - Spöri, Udo A1 - Cremer, Christoph T1 - Beyond nanosizing: an approach to shape analysis of fluorescent nanostructures by SMI-microscopy JF - Optik KW - Fluoreszenzmikroskopie Y1 - 2006 U6 - https://doi.org/https://doi.org/10.1016/j.ijleo.2005.05.006 SN - 0030-4026 VL - 117 IS - 1 SP - 26 EP - 32 PB - Elsevier BV ER - TY - JOUR A1 - Wiech, Thorsten A1 - Stein, Stefan A1 - Lachenmaier, Victoria A1 - Schmitt, Eberhard A1 - Schwarz-Finsterle, Jutta A1 - Wiech, Elisabeth A1 - Hildenbrand, Georg A1 - Werner, Martin A1 - Hausmann, Michael T1 - Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium JF - European Biophysics Journal KW - Genom KW - Krebs, Medizin Y1 - 2009 U6 - https://doi.org/https://doi.org/10.1007/s00249-009-0474-5 SN - 0175-7571 VL - 38 IS - 6 SP - 793 EP - 806 PB - Springer Science and Business Media LLC ER - TY - JOUR A1 - Kaufmann, Rainer A1 - Müller, P A1 - Hildenbrand, Georg A1 - Hausmann, Michael A1 - Cremer, Christoph T1 - Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy BT - ANALYSIS OF HER2/neu MEMBRANE PROTEIN CLUSTERS JF - Journal of Microscopy KW - Krebs, Medizin KW - Brustkrebs Y1 - 2010 U6 - https://doi.org/https://doi.org/10.1111/j.1365-2818.2010.03436.x SN - 0022-2720 VL - 242 IS - 1 SP - 46 EP - 54 PB - Wiley ER - TY - JOUR A1 - Müller, Patrick A1 - Lemmermann, Niels A. A1 - Kaufmann, Rainer A1 - Gunkel, Manuel A1 - Paech, Daniel A1 - Hildenbrand, Georg A1 - Holtappels, Rafaela A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Spatial distribution and structural arrangement of a murine cytomegalovirus glycoprotein detected by SPDM localization microscopy JF - Histochemistry and Cell Biology KW - Fluoreszenzmikroskopie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1007/s00418-014-1185-2 SN - 0948-6143 VL - 142 IS - 1 SP - 61 EP - 67 PB - Springer Science and Business Media LLC ER - TY - JOUR A1 - Falk, Martin A1 - Hausmann, Michael A1 - Lukasova, Emilie A1 - Biswas, Abin A1 - Hildenbrand, Georg A1 - Davidkova, Marie A1 - Krasavin, Evgeny A1 - Kleibl, Zdenek A1 - Falkova, Iva A1 - Jezkova, Lucie A1 - Stefancikova, Lenka A1 - Sevcik, Jan A1 - Hofer, Michal A1 - Bacikova, Alena A1 - Matula, Pavel A1 - Boreyko, Alla A1 - Vachelova, Jana A1 - Michaelidesova, Anna A1 - Kozubek, Stanislav T1 - Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part - Structuromics JF - Critical Reviews in Eukaryotic Gene Expression KW - Radiologie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1615/CritRevEukaryotGeneExpr.v24.i3.40 SN - 1045-4403 VL - 24 IS - 3 SP - 225 EP - 247 PB - Begell House ER - TY - JOUR A1 - Falk, Martin A1 - Hausmann, Michael A1 - Lukasova, Emilie A1 - Biswas, Abin A1 - Hildenbrand, Georg A1 - Davidkova, Marie A1 - Krasavin, Evgeny A1 - Kleibl, Zdenek A1 - Falkova, Iva A1 - Jezkova, Lucie A1 - Stefancikova, Lenka A1 - Sevcik, Jan A1 - Hofer, Michal A1 - Bacikova, Alena A1 - Matula, Pavel A1 - Boreyko, Alla A1 - Vachelova, Jana A1 - Michaelidesova, Anna A1 - Kozubek, Stanislav T1 - Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: PART A-Radiomics JF - Critical Reviews in Eukaryotic Gene Expression KW - Radiologie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1615/CritRevEukaryotGeneExpr.2014010313 SN - 1045-4403 VL - 24 IS - 3 SP - 205 EP - 223 PB - Begell House ER - TY - JOUR A1 - Burger, Nina A1 - Biswas, Abin A1 - Barzan, Daniel A1 - Kirchner, Anne A1 - Hosser, Hiltraud A1 - Hausmann, Michael A1 - Hildenbrand, Georg A1 - Herskind, Carsten A1 - Wenz, Frederik A1 - Veldwijk, Marlon R. T1 - A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells JF - Nanomedicine: Nanotechnology, Biology and Medicine KW - Gold KW - Nanopartikel Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1016/j.nano.2014.03.011 SN - 1549-9634 VL - 10 IS - 6 SP - 1365 EP - 1373 PB - Elsevier BV ER -