TY - JOUR A1 - Failla, Antonio Virgillo A1 - Albrecht, Benno A1 - Spöri, U. A1 - Schweitzer, A. A1 - Kroll, A. A1 - Hildenbrand, Georg A1 - Bach, M. A1 - Cremer, Christoph T1 - Nanostructure Analysis Using Spatially Modulated Illumination Microscopy JF - Complexus N2 - For an improved understanding of cellular processes, it is highly desirable to develop light optical methods for the analysis of biological nanostructures and their dynamics in the interior of three-dimensionally (3D) conserved cells. Here, important structural parameters to be considered are the topology, i.e. the mutual positions and distances, as well as the sizes of the constituting subunits. This has become possible by the development of a novel method of far-field light fluorescence microscopy, spatially modulated illumination (SMI) microscopy. Using this approach, axial distances between fluorescence-labeled targets can be measured with an accuracy close to 1 nm; their sizes can be determined down to a few tens of nanometers. This approach can be extended to the determination of 3D positions and mutual 3D distances and sizes of any number of small objects/subunits that can be discriminated due to their spectral signatures. Consequently, the new approach allows an ‘in situ nanostructure elucidation, until now regarded to be beyond the possibilities of far-field light microscopy. Application examples discussed are: colocalization/nanosizing and topological analysis of large protein-protein complexes, of nucleic acid-protein complexes (such as transcription factories), or of the highly complex DNA-protein nanostructures of which active/ inactive gene regions in the eukaryotic cell nucleus are constituted. KW - Fluoreszenzmikroskopie Y1 - 2003 U6 - https://doi.org/https://doi.org/10.1159/000070464 SN - 1424-8492 VL - 1 IS - 2 SP - 77 EP - 88 PB - S. Karger AG ER - TY - JOUR A1 - Hildenbrand, Georg A1 - Rapp, Alexander A1 - Spöri, Udo A1 - Wagner, Christian A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy JF - Biophysical Journal KW - Genom KW - Zellkern KW - Fluoreszenzmikroskopie Y1 - 2005 U6 - https://doi.org/https://doi.org/10.1529/biophysj.104.056796 SN - 0006-3495 VL - 88 IS - 6 SP - 4312 EP - 4318 PB - Elsevier BV ER - TY - JOUR A1 - Wagner, Christian A1 - Hildenbrand, Georg A1 - Spöri, Udo A1 - Cremer, Christoph T1 - Beyond nanosizing: an approach to shape analysis of fluorescent nanostructures by SMI-microscopy JF - Optik KW - Fluoreszenzmikroskopie Y1 - 2006 U6 - https://doi.org/https://doi.org/10.1016/j.ijleo.2005.05.006 SN - 0030-4026 VL - 117 IS - 1 SP - 26 EP - 32 PB - Elsevier BV ER - TY - JOUR A1 - Müller, Patrick A1 - Lemmermann, Niels A. A1 - Kaufmann, Rainer A1 - Gunkel, Manuel A1 - Paech, Daniel A1 - Hildenbrand, Georg A1 - Holtappels, Rafaela A1 - Cremer, Christoph A1 - Hausmann, Michael T1 - Spatial distribution and structural arrangement of a murine cytomegalovirus glycoprotein detected by SPDM localization microscopy JF - Histochemistry and Cell Biology KW - Fluoreszenzmikroskopie Y1 - 2014 U6 - https://doi.org/https://doi.org/10.1007/s00418-014-1185-2 SN - 0948-6143 VL - 142 IS - 1 SP - 61 EP - 67 PB - Springer Science and Business Media LLC ER -